(19)
(11) EP 0 814 091 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
02.05.2001 Bulletin 2001/18

(43) Date of publication A2:
29.12.1997 Bulletin 1997/52

(21) Application number: 97304247.6

(22) Date of filing: 17.06.1997
(51) International Patent Classification (IPC)7: C07K 16/26, C07K 17/00, C12N 5/20, G01N 33/577
(84) Designated Contracting States:
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

(30) Priority: 18.06.1996 JP 15694896

(71) Applicant: Schering Aktiengesellschaft
13353 Berlin (DE)

(72) Inventors:
  • Ikeda, Ichiro
    Mobara-shi, Chiba (JP)
  • Kono, Naoko
    Mobara-shi, Chiba (JP)
  • Makino, Tadashi
    Togane-shi, Chiba (JP)
  • Hashimoto, Yoshihide
    Funabashi-shi, Chiba (JP)

(74) Representative: Kirkham, Nicholas Andrew et al
Graham Watt & Co., Riverhead
Sevenoaks, Kent TN13 2BN
Sevenoaks, Kent TN13 2BN (GB)

   


(54) Monoclonal antiboby specifically reactive to a human growth hormone with a molecular weight of about 20,000 for use in an immunoassay


(57) A monoclonal antibody specifically reactive to a human growth hormone with a molecular weight of about 20,000, a cell line capable of producing the monoclonal antibody and an immunoassay of a human growth hormone having a molecular weight of about 20,000 using the monoclonal antibody.
An animal cell capable of producing an antibody specific to a human growth hormone having a molecular weight of 20,000 (20k hGH) was prepared from an animal immunized with 20k hGH. The animal cell was fused with a myeloma cell to produce a monoclonal antibody which specifically reacted to 20k hGH, but substantially not to 22k hGH. The monoclonal antibody was useful for immunoassay of 20k hGH.






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