(19)
(11) EP 0 821 059 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
08.03.2006 Bulletin 2006/10

(45) Mention of the grant of the patent:
21.12.2005 Bulletin 2005/51

(21) Application number: 97112672.7

(22) Date of filing: 24.07.1997
(51) International Patent Classification (IPC): 
C12N 9/12(1980.01)
C12N 15/10(1990.01)
C12N 9/96(1980.01)
 C12Q 1/68(1980.01)
C07H 21/02(1974.07)

(54)

Method for reverse transcription

Verfahren für reverse Transkription

Méthode pour transcription inverse

(84) Designated Contracting States:
CH DE FR GB IT LI NL SE

(30) Priority: 25.07.1996 JP 19632996
25.07.1996 JP 19633196

(43) Date of publication of application:
28.01.1998 Bulletin 1998/05

(60) Divisional application:
02013871.5 / 1243661

(73) Proprietors:
  • THE INSTITUTE OF PHYSICAL & CHEMICAL RESEARCH
    Wako-shi, Saitama 351-0198 (JP)
  • Kabushiki Kaisha Dnaform
    Tokyo 108-0073 (JP)

(72) Inventor:
  • Hayashizaki, Yoshihide Tsukuba Life Science Center
    Ibaragi 305 (JP)

(74) Representative: Godemeyer, Thomas et al
Sternagel, Fleischer, Godemeyer & Partner Patentanwälte An den Gärten 7
51491 Overath
51491 Overath (DE)


(56) References cited: : 
EP-A- 0 035 204
EP-A- 0 587 298
WO-A-91/09944
WO-A-94/26934
WO-A-95/20682
DE-C- 4 411 588
US-A- 5 017 492
 EP-A- 0 569 272
EP-A- 0 599 344
WO-A-94/12657
WO-A-95/14770
DE-A- 4 124 286
US-A- 4 409 200
US-A- 5 407 800
   
  • SHIMOMAYE E TE AL.: "Use of Avian Myeloblastosis virus reverse transcriptase at high temperature for sequence analysis of highly structured RNA" GENE ANALYSIS TECHNIQUES, vol. 6, no. 2, March 1989, NEW YORK US, pages 25-28, XP002056580
  • THAKAR M ET AL: "OSMOLYTE MEDIATION OF T7 DNA POLYMERASE AND PLASMID DNA STABILITY" BIOCHEMISTRY, vol. 33, no. 40, 11 October 1994, pages 12255-12259, XP002007763
  • "ENZYME STABILIZATION" ADVANCES IN BIOCHEMICAL ENGINEERING, vol. 12, 1 January 1979, NEW YORK US, pages 55-67, XP000123113
  • COLACO C ET AL: "EXTRAORDINARY STABILITY OF ENZYMES DRIED IN TREHALOSE: SIMPLIFIED MOLECULAR BIOLOGY" BIO/TECHNOLOGY., vol. 10, no. 9, 1 September 1992, NEW YORK US, pages 1007-1011, XP000568746
  • AMERSHAM INTERNATIONAL: "CATALOGUE 1994" 1993, AMERSHAM INTERNATIONAL
  • SMITH R.J.; DUERKSEN J.D.: 'GLYCEROL INHIBITION OF PURIFIED AND CHROMATIN-ASSOCIATED MOUSE LIVER HEPATOMA RNA POLYMERASE II ACTIVITY' BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS vol. 67, no. 3, 1975, pages 916 - 923
  • CARNINCI P. ET AL: 'Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA' PROC. NATL. ACAD. SCI. USA vol. 95, no. 2, 20 January 1998, pages 520 - 524
  • MWENDA J.M.: 'Biochemical characterization of a reverse transcriptase activity associated with retroviral-like particles isolated from human placental villous tissue' CELLULAR AND MOLECULAR BIOLOGY vol. 39, no. 3, May 1993, pages 317 - 328, XP008041543
  • BURTONBOY G. ET AL: 'Isolation of a C-type retrovirus from an HIV infected cell line' ARCHIVES OF VIROLOGY vol. 130, no. 3-4, 1993, pages 289 - 300, XP008041545
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).