| (19) |
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(11) |
EP 0 974 671 A8 |
| (12) |
CORRECTED EUROPEAN PATENT APPLICATION |
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published in accordance with Art. 158(3) EPC |
| (48) |
Corrigendum issued on: |
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03.07.2002 Bulletin 2002/27 |
| (43) |
Date of publication: |
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26.01.2000 Bulletin 2000/04 |
| (22) |
Date of filing: 31.07.1998 |
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| (86) |
International application number: |
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PCT/JP9803/421 |
| (87) |
International publication number: |
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WO 9915/691 (01.04.1999 Gazette 1999/13) |
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| (84) |
Designated Contracting States: |
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AT BE CH DE DK ES FI FR GB IE IT LI LU NL PT SE |
| (30) |
Priority: |
24.09.1997 JP 27647597
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| (71) |
Applicant: Sankyo Company, Limited |
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Tokyo 103-8426 (JP) |
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| (72) |
Inventors: |
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- YANO, Kazuki
Shimotsuga-gun, Tochigi 329-0511 (JP)
- KOBAYASHI, Fumie
Kawachi-gun, Tochigi 329-1104 (JP)
- GOTO, Masaaki
Shimotsuga-gun, Tochigi 329-0502 (JP)
- WASHIDA, Naohiro
Shimotsuga-gun, Tochigi 329-0518 (JP)
- TSUDA, Eisuke
Kawachi-gun, Tochigi 329-0434 (JP)
- HIGASHIO, Kanji
Kawagoe-shi, Saitama 350-0822 (JP)
- YAMADA, Yoshiji
Nagoya-shi, Aichi 458-0013 (JP)
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| (74) |
Representative: Wakerley, Helen Rachael |
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Reddie & Grose, 16 Theobalds Road
London WC1X 8PL
London WC1X 8PL (GB) |
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| |
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| (54) |
METHOD FOR DIAGNOSING BONE DYSBOLISM |
(57) A method of diagnosing metabolic bone diseases, especially osteoporosis and arthrosis
characterized by determining the concentration of osteoclastgenesis inhibitory factor
(OCIF) in humor. Monoclonal antibodies recognizing equally both of monomer type and
dimer type of OCIF. Monoclonal antibodies recognizing selectively dimer type of OCIF.
And to provide an assay kit for determination of OCIF concentration comprising the
aforementioned two antibodies recognizing different epitope of OCIF and having high
affinity showing dissociation constant of less than 2 x 10
-7 M with antigen. It is useful for a method of diagnosing metabolic bone diseases,
especially osteoporosis and arthrosis or for an assay reagent for research thereof.