Method of immortalization of human keratinocytes by down-regulation of 14-3-3 sigma expression

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EP 1 127 942 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION

(48)	Corrigendum issued on:
	16.01.2002 Bulletin 2002/03

(43)	Date of publication:
	29.08.2001 Bulletin 2001/35

(21)	Application number: 01830116.8

(22)	Date of filing: 21.02.2001

(51)	International Patent Classification (IPC)⁷: C12N 15/11, C12N 5/08, C07K 14/47, C12Q 1/02

(84)	Designated Contracting States:
	AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
	Designated Extension States:
	AL LT LV MK RO SI

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Priority:

25.02.2000 IT RM000095

(71)	Applicant: Provincia Italiana Della Congregazione Dei Figli Dell'Immacolata Concezione - Istituto Dermopatico Dell'Immacolata
	00167 Roma (IT)

(72)	Inventors:
	De Luca, Michele 00040 Ardea (IT) Dellambra, Elena 00040 Ardea (IT)

(74)	Representative: Sarpi, Maurizio
	Studio FERRARIO Via Collina, 36 00187 Roma 00187 Roma (IT)

(54)	Method of immortalization of human keratinocytes by down-regulation of 14-3-3 sigma expression

(57) Immortalised cell lines from primary human keratinocytes cell lines obtained thanks to down regulation of 14-3-3-sigma coding gene. Said immortalised cell lines are extremely useful in in vivo and in vitro studies on cancer development. Infact, immortalization is absolutely required for somatic cells transformation. It is also an object of the present invention the immortalised cell lines obtained thereof.