(19)
(11) EP 1 138 785 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
03.02.2010 Bulletin 2010/05

(45) Mention of the grant of the patent:
21.10.2009 Bulletin 2009/43

(21) Application number: 01108170.0

(22) Date of filing: 30.03.2001
(51) International Patent Classification (IPC): 
C12Q 1/68(2006.01)

(54)

Nucleic acid fragment primer or probe, and method of detecting polyhydroxyalkanoate synthesizing microorganism by using the same

Nukleinsäurefragment-Primer oder -Sonde und diese verwendendes Verfahren zum Nachweis von Polyhydroxyalkanoat-produzierenden Mikroorganismen

Fragment d'acide nucléique, amorce ou sonde et procédé les utilisant pour la détection des microorganismes synthétisant le polyhydroxyalkanoate

(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

(30) Priority: 30.03.2000 JP 2000095008

(43) Date of publication of application:
04.10.2001 Bulletin 2001/40

(73) Proprietor: CANON KABUSHIKI KAISHA
Tokyo (JP)

(72) Inventors:
  • Yano, Tetsuya, Canon Kabushiki Kaisha
    Tokyo (JP)
  • Imamura, Takeshi, Canon Kabushiki Kaisha
    Tokyo (JP)
  • Suda, Sakae, Canon Kabushiki Kaisha
    Tokyo (JP)
  • Honma, Tsutomu, Canon Kabushiki Kaisha
    Tokyo (JP)

(74) Representative: Weser, Wolfgang 
Weser & Kollegen Patentanwälte Radeckestrasse 43
81245 München
81245 München (DE)


(56) References cited: : 
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WO-A-98/33893
US-A- 5 474 917
US-A- 5 597 710
US-A- 5 750 848
 EP-A- 0 844 305
JP-T- 06 279 492
US-A- 5 500 341
US-A- 5 639 612
   
  • DATABASE EBI [Online] 3 March 2000 (2000-03-03) MARRA M. ET AL.: "The WashU-HHMI Mouse EST project" Database accession no. AA921165 XP002266259
  • LOPEZ NANCY I ET AL: "Detection of reserve polymer synthesis genes in natural bacterial populations." FEMS MICROBIOLOGY ECOLOGY, vol. 22, no. 2, 1997, pages 129-136, XP001155332 ISSN: 0168-6496
  • PEOPLES O P ET AL: "POLY-BETA-HYDROXYBUTYRATE BIOSYNTHESIS IN ALCALIGENES EUTROPHUS H16CHARACTERIZATION OF THE GENES ENCODING BETA--KETOTHIOLASE AND ACETOACETYL-COA REDUCTASE" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 264, no. 26, 16 September 1989 (1989-09-16), pages 15293-15297, XP002934260 ISSN: 0021-9258
  • HUISMAN ET AL: "Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 266, no. 4, 5 February 1991 (1991-02-05), pages 2191-2198, XP002087431 ISSN: 0021-9258
  • TIMM ET AL: "Cloning and molecular analysis of the poly(3-hydroxyalkanoic acid) gene locu of Pseudomonas aeruginosa PAO1" EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 1, no. 209, 1 October 1992 (1992-10-01), pages 15-30, XP002077729 ISSN: 0014-2956
  • MATSUSAKI HIROMI ET AL: "Cloning and molecular analysis of the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyalkanoa te) biosynthesis genes in Pseudomonas sp. strain 61-3" JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 180, no. 24, December 1998 (1998-12), pages 6459-6467, XP002167124 ISSN: 0021-9193
  • SHEU DER-SHYAN ET AL: "Rapid detection of polyhydroxyalkanoate-accumulating bacteria isolated from the environment by colony PCR." MICROBIOLOGY (READING), vol. 146, no. 8, August 2000 (2000-08), pages 2019-2025, XP002255569 ISSN: 1350-0872
  • SOLAIMAN D K Y ET AL: "Rapid and specific identification of medium-chain-length polyhydroxyalkanoate synthase gene by polymerase chain reaction." APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 53, no. 6, June 2000 (2000-06), pages 690-694, XP002255570 ISSN: 0175-7598
  • SCHEMBRI M A ET AL: "CLONING AND ANLYSIS OF THE POLYHYDROXYALKANOIC ACID SYNTHASE GENE FROM AN ACINETOBACTER SP.: EVIDENCE THAT THE GENE IS BOTH PLASMID AND CHROMOSOMALLY LOCATED" FEMS MICROBIOLOGY LETTERS, AMSTERDAM, NL, vol. 118, no. 1/2, 1 May 1994 (1994-05-01), pages 145-152, XP000674914 ISSN: 0378-1097
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).