(19)
(11) EP 1 141 239 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
07.10.2009 Bulletin 2009/41

(45) Mention of the grant of the patent:
12.08.2009 Bulletin 2009/33

(21) Application number: 99967431.0

(22) Date of filing: 17.12.1999
(51) International Patent Classification (IPC): 
C12N 5/00(2006.01)
C12N 15/00(2006.01)
C12N 15/63(2006.01)
C12N 15/74(2006.01)
C07H 21/04(2006.01)
C12N 5/02(2006.01)
C12N 15/09(2006.01)
C12N 15/70(2006.01)
C07H 21/02(2006.01)
(86) International application number:
PCT/US1999/030277
(87) International publication number:
WO 2000/036088 (22.06.2000 Gazette 2000/25)

(54)

IMPROVED METHODS FOR INSERTION OF NUCLEIC ACIDS INTO CIRCULAR VECTORS

VERBESSERTE VERFAHREN ZUR EINSCHLEUSUNG VON NUKLEINSÄUREN IN RINGFÖRMIGE VEKTOREN

PROCEDES AMELIORES D'INSERTION D'ACIDES NUCLEIQUES DANS DES VECTEURS CIRCULAIRES


(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

(30) Priority: 17.12.1998 US 213834

(43) Date of publication of application:
10.10.2001 Bulletin 2001/41

(60) Divisional application:
09010345.8

(73) Proprietor: Romantchikov, Yuri
Hagerstown MD 21742 (US)

(72) Inventor:
  • Romantchikov, Yuri
    Hagerstown MD 21742 (US)

(74) Representative: Vossius & Partner 
Siebertstraße 4
81675 München
81675 München (DE)


(56) References cited: : 
EP-A- 0 773 294
US-A- 5 844 084
WO-A-94/18333
   
  • BOYD A CHRISTOPHER: "Turbo cloning: A fast, efficient method for cloning PCR products and other blunt-ended DNA fragments into plasmids" NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 4, 1993, pages 817-821, XP002184738 ISSN: 0305-1048
  • UPCROFT P ET AL: "RAPID AND EFFICIENT METHOD FOR CLONING OF BLUNT-ENDED DNA FRAGMENTS" GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 51, no. 1, 1987, pages 69-75, XP002184737 ISSN: 0378-1119
  • DAMAK S ET AL: "A simple two-step method for efficient blunt-end ligation of DNA fragments" BIOTECHNIQUES, vol. 15, no. 3, 1993, pages 448-450, 452, XP009043044 ISSN: 0736-6205
  • SHUMAN S: "DNA strand transfer reactions catalyzed by vaccinia topoisomerase I" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 267, no. 12, 25 April 1992 (1992-04-25), pages 8620-8627, XP002205832 ISSN: 0021-9258
  • SHUMAN STEWART: "Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 269, no. 51, 23 December 1994 (1994-12-23), pages 32678-32684, XP002164562 ISSN: 0021-9258
  • MIKI T. ET AL.: 'An efficient directional cloning system to construct cDNA libraries containing full-length inserts at high frequency' vol. 83, 1989, pages 137 - 146, XP002925975
  • ASLANIDIS C. ET AL.: 'Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products' PCR METHODS AND APPLICATIONS vol. 4, no. 3, December 1994, pages 172 - 177, XP002925976
  • FUJIMAKI K. ET AL.: 'DNA topoisomerase II inhibitors enhance random integration of transfected vectors into human chromosomes' SOMATIC CELL AND MOLECULAR GENETICS vol. 22, no. 4, July 1996, pages 279 - 290, XP002925977
  • MANIATIS A. ET AL.: 'Molecular Cloning a Laboratory Manual' COLD SPRING HARBOR LABORATORY 1982, pages 17 - 20, XP002925978
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).