(19)

(11)

EP 1 147 198 A1

(12)

(43) Date of publication: 24.10.2001 Bulletin 2001/43 (21) Application number: 98966046.9 (22) Date of filing: 23.12.1998

(51) International Patent Classification (IPC)7: C12N 15/52, C12N 9/00, C12P 13/08 (86) International application number: PCT/US9827/301 (87) International publication number: WO 0039/305 (06.07.2000 Gazette 2000/27)

(84) Designated Contracting States: AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE (71) Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY Cambridge, MA 02139 (US)	(72) Inventors: SINSKEY, Anthony J. Boston, MA 02115 (US) LESSARD, Philip A. Framingham, MA 01701 (US) WILLIS, Laura B. Cambridge, MA 02139 (US) (74) Representative: Lee, Nicholas John et al Kilburn & Strode,20 Red Lion Street London WC1R 4PJ London WC1R 4PJ (GB)