(19)
(11) EP 1 165 768 A1

(12)

(43) Date of publication:
02.01.2002 Bulletin 2002/01

(21) Application number: 00912216.9

(22) Date of filing: 13.03.2000
(51) International Patent Classification (IPC)7C12N 15/10, C12N 15/62, C12N 15/70, C12N 15/85, C12N 9/90, C07K 14/47, C12Q 1/68
(86) International application number:
PCT/US0006/560
(87) International publication number:
WO 0056/878 (28.09.2000 Gazette 2000/39)
(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
Designated Extension States:
AL LT LV MK RO SI

(30) Priority: 19.03.1999 US 125126 P

(71) Applicant: INVITROGEN CORPORATION
Carlsbad, CA 92008 (US)

(72) Inventors:
  • HEYMAN, John, A.
    Carlsbad, CA 92008 (US)
  • COMISKEY, John, D.
    Carlsbad, CA 92008 (US)
  • MARCIL, Robert
    Carlsbad, CA 92008 (US)
  • PHELAN, Dorothy
    Carlsbad, CA 92008 (US)

(74) Representative: Cornish, Kristina Victoria Joy et al
Kilburn & Strode, 20 Red Lion Street
London WC1R 4PJ
London WC1R 4PJ (GB)

   


(54) METHODS OF OBTAINING FULL-LENGTH NUCLEIC ACID SEQUENCES USING $i(E. COLI) TOPOISOMERASE III AND ITS HOMOLOGS