(19)
(11) EP 1 215 286 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
26.06.2002 Bulletin 2002/26

(43) Date of publication A2:
19.06.2002 Bulletin 2002/25

(21) Application number: 01129615.9

(22) Date of filing: 12.12.2001
(51) International Patent Classification (IPC)7: C12Q 1/68
(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
Designated Extension States:
AL LT LV MK RO SI

(30) Priority: 14.12.2000 JP 2000380465

(71) Applicant: Hitachi Software Engineering Co., Ltd.
Yokohama-shi, Kanagawa 231-8475 (JP)

(72) Inventors:
  • Nakao, Motonao, Hitachi Software Engineer. Co. Ltd
    Naka-ku, Yokohama-shi, Kanagawa 231-8475 (JP)
  • Mizuno, Katsuya, Hitachi Software Enginee. Co. Ltd
    Naka-ku, Yokohama-shi, Kanagawa 231-8475 (JP)
  • Yoshii, Junji, Hitachi Software Engineer. Co., Ltd
    Naka-ku, Yokohama-shi, Kanagawa 231-8475 (JP)
  • Asai, Akihiro, Hitachi Software Engineer. Co., Ltd
    Naka-ku, Yokohama-shi, Kanagawa 231-8475 (JP)

(74) Representative: Liesegang, Roland, Dr.-Ing. 
FORRESTER & BOEHMERT Pettenkoferstrasse 20-22
80336 München
80336 München (DE)

   


(54) Detection method and detection kit for PCR-amplified base sequences


(57) Determination of PCR products amplified by PCR can be readily and surely conducted even if multiple types of primers are used therein. By using a biochip of a glass slide with primers for the PCR implanted thereon, competitive PCR is performed in one tube with the multiple types of primers, and amplified PCR products are detected by use of the primers on the biochip.






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