(19)
(11) EP 1 251 168 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
11.08.2004 Bulletin 2004/33

(45) Mention of the grant of the patent:
23.06.2004 Bulletin 2004/26

(21) Application number: 02008545.2

(22) Date of filing: 16.04.2002
(51) International Patent Classification (IPC)7: C12N 15/10, C12N 15/68

(54)

Method for improving the stability of linear DNA in cell-free in vitro transcription/translation systems

Verfahren zur Verbesserung der Stabilität linearer DNS in zellfreien in vitro Transkriptions/Translations-Systemen

Procede pour l'amélioration de la stabilité d'ADN lineaire dans des systemes in vitro de transcription/translation acellulaires

(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

(30) Priority: 18.04.2001 DE 10119006

(43) Date of publication of application:
23.10.2002 Bulletin 2002/43

(73) Proprietors:
  • Roche Diagnostics GmbH
    68305 Mannheim (DE)
    Designated Contracting States:
    DE 
  • F. HOFFMANN-LA ROCHE AG
    4070 Basel (CH)
    Designated Contracting States:
    AT BE CH CY DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR 

(72) Inventors:
  • Watzele, Manfred, Dr.
    82362 Weilheim (DE)
  • Hoffmann, Thomas
    68535 Neu-Edingen (DE)
  • Nemetz, Cordula, Dr.
    82515 Wolfratshausen (DE)
  • Heindl, Dieter, Dr.
    82327 Tutzing (DE)
  • Metzler, Thomas, Dr.
    80337 München (DE)
  • Mutter, Wolfgang, Dr.
    82347 Bernried (DE)


(56) References cited: : 
WO-A-94/24303
   
  • WIRTH R ET AL: "FACTORS MODULATING TRANSCRIPTION AND TRANSLATION IN VITRO OF RIBOSOMAL PROTEIN S20 AND ISOLEUCYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI" EUROPEAN JOURNAL OF BIOCHEMISTRY, BERLIN, DE, vol. 114, 1981, pages 429-437, XP002932536 ISSN: 0014-2956
  • JACKSON M ET AL: "Enhanced polypeptide synthesis programmed by linear DNA fragments in cell-free extracts lacking exonuclease V." FEBS LETTERS. NETHERLANDS 14 NOV 1983, vol. 163, no. 2, 14 November 1983 (1983-11-14), pages 221-224, XP008005956 ISSN: 0014-5793
  • PATENT ABSTRACTS OF JAPAN vol. 2000, no. 22, 9 March 2001 (2001-03-09) -& JP 2001 136971 A (NIPPON FLOUR MILLS CO LTD), 22 May 2001 (2001-05-22)
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).