| (19) |
 |
|
(11) |
EP 1 288 294 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
|
23.04.2003 Bulletin 2003/17 |
| (43) |
Date of publication A2: |
|
05.03.2003 Bulletin 2003/10 |
| (22) |
Date of filing: 26.04.2002 |
|
|
| (84) |
Designated Contracting States: |
|
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR |
|
Designated Extension States: |
|
AL LT LV MK RO SI |
| (30) |
Priority: |
31.08.2001 US 943857
|
| (71) |
Applicant: Academia Sinica |
|
Nan-Kang, Taipei (TW) |
|
| (72) |
Inventors: |
|
- Tang, Shye-Jye
Shrlin Chiu, Taipei Taiwan 111 R.O.C. (TW)
- Lee, Guan-Chiun
Shinyi Chiu, Taipei Taiwan 110 R.O.C. (TW)
- Shaw, Jei-Fu
Nangang Chiu, Taipei Taiwan, 115 R.O.C. (TW)
|
| (74) |
Representative: Epping Hermann & Fischer |
|
Ridlerstrasse 55
80339 München
80339 München (DE) |
|
| |
|
| (54) |
Recombinant Candida rugosa lipases |
(57) The present invention features an isolated nucleic acid encoding a mutant
Candida rugosa lipase, wherein the mutant nucleic acid is 80% identical to a wilt-type DNA encoding
a
Candida rugosa lipase, and where at least 12 of the CTG codons, corresponding to serine in the wild-type
DNA, have been replaced by a universal serine codon. The
Candida rugosa lipase can be lipase 1,2,3,5 or 8.