(19)
(11) EP 1 298 212 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
20.12.2006 Bulletin 2006/51

(45) Mention of the grant of the patent:
02.11.2006 Bulletin 2006/44

(21) Application number: 02256756.4

(22) Date of filing: 27.09.2002
(51) International Patent Classification (IPC): 
C12N 15/54(2006.01)
C12N 15/63(2006.01)
C12N 9/22(2006.01)
 C12N 15/55(2006.01)
C12N 9/10(2006.01)
C12N 5/10(2006.01)

(54)

Method for cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease

Verfahren zur Klonierung und Exprimierung von BsmBI-Restriktionsendonuklease und BsmBI-Methylase in E. coli, und zur Purifizierung von BsmBI-Endonuklease

Méthode de clonage et d'expression de l'endonucléase de réstriction BsmBI et de la méthylase BsmBI dans E. coli, et procédé de purification de l'endonuclease BsmBI

(84) Designated Contracting States:
DE FR GB
Designated Extension States:
LT

(30) Priority: 28.09.2001 US 966997

(43) Date of publication of application:
02.04.2003 Bulletin 2003/14

(73) Proprietor: NEW ENGLAND BIOLABS, INC.
Ipswich, MA 01938-2723 (US)

(72) Inventors:
  • Xu, Shuang-Yong
    Lexington, Massachusetts 02420 (US)
  • Dore, Andrew
    Nr. Uckfield, E. Sussex TN22 5NH (GB)
  • Hume, Adam
    Lewiston, Maine 04240 (US)
  • Pellitier, John
    Amesbury, Massachusetts 01913 (US)
  • Zhou, Jing
    Beverly, Massachusetts 01915 (US)

(74) Representative: Davies, Jonathan Mark 
Reddie & Grose 16 Theobalds Road
London WC1X 8PL
London WC1X 8PL (GB)


(56) References cited: : 
US-A- 5 137 823
 US-A- 5 200 333
   
  • "BsmBI Restriction endonuclease description" NEW ENGLAND BIOLABS CATALOGUE 1998/99, 1998, XP002245974 US
  • MIYAHARA M ET AL: "Isolation and characterisation of new restriction endonucleases from Vibrio parahaemolyticus: VpaK32I enzyme with the class-IIS heptanucleotide specificity, GCTCTTCN1/N4" GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 117, 1992, pages 103-106, XP002103321 ISSN: 0378-1119
  • PIEKAROWICZ A ET AL: "A new method for the rapid identification of genes encoding restriction and modification enzymes" NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 19, no. 8, 1991, pages 1831-1835, XP002103320 ISSN: 0305-1048
  • WILSON G G: "CLONED RESTRICTION-MODIFICATION SYSTEMS - A REVIEW" GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 74, no. 1, 1988, pages 281-289, XP000165660 ISSN: 0378-1119
  • BITINAITE J ET AL: "ESP-3-I A NOVEL TYPE IIS RESTRICTION ENDONUCLEASE FROM HAFNIA-ALVEI THAT RECOGNIZES THE SEQUENCE 5' CGTCTC-N-1-5-3'" NUCLEIC ACIDS RESEARCH, vol. 19, no. 18, 1991, page 5076 XP002245975 ISSN: 0305-1048
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).