(19)
(11) EP 1 298 991 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
13.01.2016 Bulletin 2016/02

(45) Mention of the grant of the patent:
28.10.2015 Bulletin 2015/44

(21) Application number: 01937288.7

(22) Date of filing: 09.05.2001
(51) International Patent Classification (IPC): 
G01N 15/14(2006.01)
C12M 3/00(2006.01)
 A01N 1/02(2006.01)
(86) International application number:
PCT/US2001/015150
(87) International publication number:
WO 2001/085913 (15.11.2001 Gazette 2001/46)

(54)

METHOD FOR ISOLATING X-CHROMOSOME AND Y-CHROMOSOME BEARING POPULATIONS OF SPERMATOZOA

VERFAHREN ZUR TRENNUNG VON X- UND Y-CHROMOSOMEN TRAGENDEN POPULATIONEN VON SPERMATOZOEN

PROCÉDÉ DE SÉPARATION DE POPULATIONS DE SPERMATOZOIDES PORTEURS DE CHROMOSOMES X ET Y

(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

(30) Priority: 09.05.2000 US 203089 P
12.10.2000 US 239752 P
10.02.2001 US 267571 P

(43) Date of publication of application:
09.04.2003 Bulletin 2003/15

(60) Divisional application:
10176889.3 / 2258168
10176904.0 / 2258169
10176908.1 / 2258170
10176910.7 / 2258171
10176917.2 / 2258172
10176919.8 / 2258173
10176924.8 / 2258174

(73) Proprietor: XY, LLC
Navasota, TX 77868 (US)

(72) Inventors:
  • EVANS, Kenneth, M.
    Fort Collins, CO 80526 (US)
  • VAN MUNSTER, Erik, B.
    NL-1015 GP Amsterdam (NL)

(74) Representative: Kador & Partner 
Corneliusstraße 15
80469 München
80469 München (DE)


(56) References cited: : 
EP-A1- 0 140 616
WO-A1-01/51612
US-A- 5 017 497
US-A- 5 891 734
US-A- 6 154 276
 WO-A-91/05236
US-A- 4 804 891
US-A- 5 135 759
US-A- 5 985 216
   
  • JOHNSON L A ET AL: "MODIFICATION OF A LASER-BASED FLOW CYTOMETER FOR HIGH- RESOLUTION DNA ANALYSIS OF MAMMALIAN SPERMATOZOA" CYTOMETRY, ALAN R. LISS, INC, vol. 7, no. 3, May 1986 (1986-05), pages 268-273, XP002925095 ISSN: 0196-4763
  • JOHNSON L A ET AL: "SEX PRESELECTION: HIGH-SPEED FLOW CYTOMETRIC SORTING OF X AND Y SPERM FOR MAXIMUM EFFICIENCY" THERIOGENOLOGY, LOS ALTOS, CA, US, vol. 52, no. 8, 1999, pages 1323-1341, XP001025636 ISSN: 0093-691X
  • VAN MUNSTER ET AL.: "difference in volume of X and Y chromosome bearing bovine sperm heads matches difference in DNA content" CYTOMETRY, 1999, pages 125-128, XP002415352 us
  • VAN MUNSTER: "INTERFEROMETRY IN FLOW TO SORT UNSTAINED X AND Y CHROMOSOME BEARING BULL SPERMATOZOA" CYTOMETRY, vol. 47, 2002, pages 192-199, XP002415476
  • BEYHAN ET AL.: 'Sexual dimorphism in IVM-IVF bovine embryos produced from X and chromosome-bearing spermatozoa sorted by high speed flow cytometry' vol. 52, no. 1, 01 July 1999, pages 35 - 48, XP000985524
  • JOHNSON L A ET AL: "MODIFICATION OF A LASER-BASED FLOW CYTOMETER FOR HIGH- RESOLUTION DNA ANALYSIS OF MAMMALIAN SPERMATOZOA", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 7, no. 3, 1 May 1986 (1986-05-01), pages 268-273, XP002925095, ISSN: 0196-4763, DOI: 10.1002/CYTO.990070307
  • shapiro: "practical flow cytometry (third edition)", 1995, new-york 244870 * page 129, column 1, paragraph 2 * * page 131, column 1, paragraph 2 *
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).