(19)
(11) EP 1 313 876 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
28.02.2007 Bulletin 2007/09

(45) Mention of the grant of the patent:
03.01.2007 Bulletin 2007/01

(21) Application number: 01974181.8

(22) Date of filing: 13.08.2001
(51) International Patent Classification (IPC): 
C12Q 1/42(2006.01)
C12Q 1/34(2006.01)
 G01N 33/573(2006.01)
C12Q 1/48(2006.01)
(86) International application number:
PCT/EP2001/009354
(87) International publication number:
WO 2002/014543 (21.02.2002 Gazette 2002/08)

(54)

PROCESS FOR DETECTING ACETYLASE OR DEACETYLASE ACTIVITY IN AN IMMUNOASSAY

VERFAHREN ZUM NACHWEIS VON ACETYLASE ODER DEACETYLASE AKTIVITÄT IN EINEM IMMUNOASSAY

PROCEDE DE DETECTION D'ACTIVITE D'UN ACETYLASE OU DEACETYLASE DANS UN DOSAGE IMMUNOLOGIQUE

(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

(30) Priority: 11.08.2000 EP 00117457
24.08.2000 EP 00118431
18.10.2000 EP 00122707
21.12.2000 EP 00128176

(43) Date of publication of application:
28.05.2003 Bulletin 2003/22

(73) Proprietor: Evotec AG
22525 Hamburg (DE)

(72) Inventors:
  • KRÄMER, Joachim
    25474 Ellerbek (DE)
  • PEIKER, Christine
    25469 Halstenbek (DE)
  • HENCO, Karsten
    40699 Erkrath (DE)

(74) Representative: Hertz, Oliver 
v. Bezold & Sozien, Akademiestrasse 7
80799 München
80799 München (DE)


(56) References cited: : 
WO-A-99/29894
   
  • KHOKHLATCHEV ANDREI ET AL: "Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria: Efficient synthesis of active protein kinase" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 272, no. 17, 1997, pages 11057-11062, XP002163437 ISSN: 0021-9258
  • BORGNE A & MEIJER L: "Sequential dephosphorylation of p34cdc2 on Thr-14 and Tyr-15 at the prohase/metaphase transition" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 271, no. 44, 1996, pages 27847-27854, XP002162637 ISSN: 0021-9258
  • CLAYTON ALISON L ET AL: "Phosphoacetylation of histone H3 on c-fos- and c-jun-associated nucleosomes upon gene activation." EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 19, no. 14, 17 July 2000 (2000-07-17), pages 3714-3726, XP002225679 ISSN: 0261-4189
  • SEETHALA RAMAKRISHNA ET AL: "A fluorescence polarization competition immunoassay for tyrosine kinases" ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 255, 15 January 1998 (1998-01-15), pages 257-262, XP002150326 ISSN: 0003-2697
  • WU J J ET AL: "IDENTIFICATION OF A HIGH-AFFINITY ANTI-PHOSPHOSERINE ANTIBODY FOR THE DEVELOPMENT OF A HOMOGENEOUS FLOURESCENCE POLARIZATION ASSAY OF PROTEIN KINASE C" JOURNAL OF BIOMOLECULAR SCREENING, LARCHMONT, NY, US, vol. 5, no. 1, February 2000 (2000-02), pages 23-30, XP000992190 ISSN: 1087-0571
  • CHEN TING ET AL: "Interaction of phosphorylated Fc-epsilon-RI-gamma immunoglobulin receptor tyrosine activation motif-based peptides with dual and single SH2 domains of p72-syk. Assessment of binding parameters and real time binding kinetics." JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 271, no. 41, 1996, pages 25308-25315, XP002225680 ISSN: 0021-9258
  • DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; August 1999 (1999-08) BARANCIK MIROSLAV ET AL: "Okadaic acid and anisomycin are protective and stimulate the SAPK/JNK pathway." Database accession no. PREV199900492152 XP002225692 & JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, vol. 34, no. 2, August 1999 (1999-08), pages 182-190, ISSN: 0160-2446
   
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