(19)
(11) EP 1 327 683 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
07.01.2004 Bulletin 2004/02

(43) Date of publication A2:
16.07.2003 Bulletin 2003/29

(21) Application number: 03075150.7

(22) Date of filing: 15.05.1995
(51) International Patent Classification (IPC)7: C12N 15/52, C12N 9/00, A61K 31/70, C12Q 1/68, C12N 15/11, C12N 5/10, A61K 31/713, A61P 35/00
(84) Designated Contracting States:
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

(30) Priority: 13.05.1994 US 242665

(62) Application number of the earlier application in accordance with Art. 76 EPC:
95920437.1 / 0759992

(71) Applicants:
  • The Government of the United States of America, as represented by the Secretary Department of Health and Human Services
    Rockville, MD 20852-3804 (US)
  • Northern Illinois University
    DeKalb, IL 60115 (US)

(72) Inventors:
  • Hampel, Arnold
    Dekalb, IL 60115 (US)
  • Dipaolo, Joseph
    Bethesda, MD 20817 (US)
  • Siwkowski, Andrew
    Sycamore, IL 60178 (US)

(74) Representative: Van Malderen, Joelle et al
Office Van Malderen, Place Reine Fabiola 6/1
1083 Bruxelles
1083 Bruxelles (BE)

   


(54) Human papilloma virus inhibition by a hairpin ribozyme


(57) The present invention is related to synthetic ribozymes, i.e. ribozymes including a hairpin portion, binding sites for binding to a human papilloma virus (HPV) after viral base 419 or 434, respectively, and cleavage sites for cleaving HPV virus are disclosed. The invention also includes a diagnostic assay for determining the presence of HPV-16 in tissues based on PCR amplification.






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