(19)
(11) EP 1 358 346 A2

(12)

(88) Date of publication A3:
04.09.2003

(43) Date of publication:
05.11.2003 Bulletin 2003/45

(21) Application number: 01924120.7

(22) Date of filing: 07.03.2001
(51) International Patent Classification (IPC)7C12Q 1/68, G06F 19/00
(86) International application number:
PCT/US0107/101
(87) International publication number:
WO 0106/6799 (13.09.2001 Gazette 2001/37)
(84) Designated Contracting States:
AT BE CH CY DE DK FR GB IT LI NL

(30) Priority: 07.03.2000 US 187537 P

(71) Applicant: E.I. DUPONT DE NEMOURS AND COMPANY
Wilmington, DE 19808 (US)

(72) Inventors:
  • SCHAFFER, Michael Edward
    Boothwyn, PA 19061 (US)
  • TSENG, Susan, Yen-tee
    Hockessin, DE 19707 (US)

(74) Representative: Webber, Philip Michael 
Frank B. Dehn & Co.,179 Queen Victoria Street
London EC4V 4EL
London EC4V 4EL (GB)

   


(54) REAL TIME QUANTITATIVE PCR WITH INTERCALATING DYE FOR SINGLE AND MULTIPLEX TARGET DNA