(19)
(11) EP 1 404 825 A2

(12)

(88) Date of publication A3:
31.07.2003

(43) Date of publication:
07.04.2004 Bulletin 2004/15

(21) Application number: 02747439.4

(22) Date of filing: 26.06.2002
(51) International Patent Classification (IPC)7: C12N 9/16
(86) International application number:
PCT/EP2002/007060
(87) International publication number:
WO 2003/002736 (09.01.2003 Gazette 2003/02)
(84) Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

(30) Priority: 27.06.2001 EP 01115424

(71) Applicants:
  • Roche Diagnostics GmbH
    68305 Mannheim (DE)

    DE 
  • F. HOFFMANN-LA ROCHE AG
    4070 Basel (CH)

    AT BE CH CY DK ES FI FR GB GR IE IT LU MC NL PT SE TR LI 

(72) Inventors:
  • SHAO, Zhixin
    82377 Penzberg (DE)
  • KRATZSCH, Peter
    82387 Antdorf (DE)
  • SCHMUCK, Rainer
    83671 Benediktbeuern (DE)
  • VON DER ELTZ, Herbert
    82362 Weilheim (DE)
  • KENKLIES, Janet
    82377 Penzberg (DE)

   


(54) A WALK-THROUGH TECHNIQUE FOR i IN VITRO /i RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES