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(11) | EP 1 405 668 A1 |
| (12) | EUROPEAN PATENT APPLICATION |
| published in accordance with Art. 158(3) EPC |
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| (54) | NOVEL SEPARATION AGENT FOR SEPARATING OPTICAL ISOMER AND METHOD FOR PREPARATION THEREOF |
| (57) A method of producing a novel separating agent for separating enantiomeric isomers,
characterized by including: a step of supporting an optically active polymer compound
and a compound having an asymmetric structure of a molecular weight of 1,000 or less
on a carrier using a solvent; a step of removing the solvent; and a step of removing
the compound having the asymmetric structure of the molecular weight of 1,000 or less
by washing. |
Technical Field
[0001] The present invention relates to a novel separating agent for separating enantiomeric isomers, a method of producing the same, and a separation method for enantiometric isomers using the same, in particular, a separation method for enantiomeric isomers with chromatography. The present invention provides a separation technique of the enantiomeric isomers, which enable optical resolution of chiral compounds with high separation coefficient in analyses of medicines, foods, agricultural chemicals, fragrant materials, and the like.
Background Art
[0002] Many organic compounds have enantiomeric isomers having completely the same physical and chemical properties, but differing in a physiological activity. This is attributable to the following. In most cases, proteins or glucides per se constituting a living being are composed of only one of the enantiomeric isomers, and for this reason, any difference arises in the manner of acting on the other enantiomeric isomer, resulting in a difference in the physiological activity. In particular, in many cases, there are significant differences in medical property and toxicity between the enantiomeric isomers of the pharmaceuticals, and this problem is recognized as a significant problem in the field of pharmaceuticals. The Ministry of Health, Labour and Welfare of Japan prescribes in the Guideline for the Production of Medicines that "in the case where the drug is a racemi form, it is desirable that studies on the dynamic behaviors of absorption, distribution, metabolism and excretion be made on each enantiomeric isomer".
[0003] Since the enantiomeric isomers have completely the same physical and chemical properties, for example, the physical properties such as a boiling point, a melting point or a solubility, as described above, they cannot be analyzed by ordinary separation means. For this reason, extensive investigations have been made on techniques for analyzing a wide variety of enantiomeric isomers in a simple manner and with high precision. As a result, an optical resolution method by high performance liquid chromatography (HPLC) , in particular, an optical resolution method using an enantiomeric isomer separation column for HPLC has been developed as an analyzing method that meets those requirements. The enantiomeric isomer separation column defined herein uses an asymmetry identifying agent itself or a chiral immobilizing phase having the asymmetry identifying agent supported on a suitable carrier.
[0004] As examples of the chiral immobilizing phase, optically active poly(triphenylmethyl methacrylate) (cf., JP-A 57-150432), cellulose and amylose derivatives (cf., JP-A 60-40952, JP-A 60-108751, JP-A 60-142930 and JP-A 63-178101), ovomucoid which is a protein (JP-A 63-307829), and the like have been developed. It is known that, of those many chiral immobilizing phases for HPLC, a column for enantiomeric isomer separation having the cellulose or amylose derivatives supported on silica gel has a high asymmetry identification ability to a very wide variety of compounds (for example, Okamoto et al., Angew. Chem. Int. Ed., 1998, 37, 1020).
[0005] In the case of aiming at analyses such as an optical purity measurement, it has been desired that as many unidentified enantiomeric isomer compounds as possible can be separated by as few kinds as possible of enantiomeric isomer separation columns. As a result, the above-mentioned column for enantiomeric isomer separation having the cellulose or amylose derivatives supported on the silica gel has been accepted as practical separation media.
[0006] In recent years, studies on a liquid preparative chromatography for optically active substances on an industrial scale in a combination of a chiral immobilizing phase for HPLC and a simulated moving bed method have been developed (Phrarm. Tech. Japan, 12, 43 (1996). In such studies, not only analysis, but also preparative separation, namely, chromatographic separation as production means, are noted.
[0007] For that purpose, in order to not only merely perform base line separation, but improve a productivity of the preparative chromatography and decrease a production cost, it is demanded to develop a chiral immobilizing phase that enables the further separation of a target compound for the limited, specified preparative separation, that is, has a value of separation coefficient α as high as possible.
[0008] On the other hand, a molecular imprinting method is known as a method of specifically identifying the specific target compound. The most popular method in general production methods for a molecular template is that the target compound (guest) and a monomer for performing non-conjugated-bond-type interaction therewith are reacted in a test tube using a crosslinking agent or the like for polymerization, thereby obtaining a polymer compound (host). Further, a method of obtaining a host by mixing the guest and a polymer and subjecting the polymer to a crosslinking reaction in an interaction state is known (for example, G. Wulff et al., Angew. Chem., 1972, 84, 364).
[0009] A filler for chromatography obtained by such molecular imprinting has a high identification ability to a guest. However, it is known that sucha filler is extremely high in degree of adsorption to a guest, and as a result, the corresponding elution peak shows a widely extended form. This is not satisfactory in terms of chromatography efficiency. Further, the polymer compound obtained by the molecular imprinting method cannot include a dissolution operation that may decompose a prepared template. For this reason, the polymer compound is prepared into the filler for chromatography by pulverization processing or the like. However, an operation such as classification is complicated, and a particle size is not uniform, resulting in decrease in chromatography efficiency. For the above reason, the filler for chromatography obtained by the technique using the molecular imprinting method has not been yet put into practical use.
[0010] A purpose of the present invention is to a process for producing a novel separating agent for separating enantiomeric isomers to obtain a separating agent for separating enantiomeric isomers, which is greatly improved in separation efficiency to an objective compounds for separation.
[0011] Another purpose of the present invention is to provide a separating agent for separating enantiomeric isomers, which has a greatly improved separation performance to a compound to be separated and can separate compounds that could not conventionally be separated.
[0012] Still another purpose of the present invention is to provide an immobilizing phase for chromatography or an immobilizing phase for continuous liquid preparative chromatography, using the separating agent for separating enantiomeric isomers, and a separation method for enantiomeric isomers using the separating agent for separating enantiomeric isomers.
Disclosure of the Invention
[0013] The present inventors have found that a separation ability is greatly improved by improving a separating agent for separating enantiomeric isomers in place of selective use of a general-purpose developing solvent conventionally used, specifically improving the separating agent for separating enantiomeric isomers using a compound having an asymmetric structure of a molecular weight of 1,000 or less, as a method of increasing the separation ability of the separating agent for separating enantiomeric isomers.
[0014] More specifically, according to the present invention, there is provided a method of producing a novel separating agent for separating enantiomeric isomers, characterized by including adding a compound having an asymmetric structure of a molecular weight of 1, 000 or less in supporting an optically active polymer compound on a carrier.
[0015] Further, according to the present invention, there is provided a method of producing a novel separating agent for separating enantiomeric isomers, characterized by including: a step of supporting an optically active polymer compound and a compound having an asymmetric structure of a molecular weight of 1,000 or less on a carrier using a solvent; and a step of removing the solvent.
[0016] Further, according to the present invention, there is provided a method of producing a novel separating agent for separating enantiomeric isomers, characterized by including: a step of supporting an optically active polymer compound and a compound having an asymmetric structure of a molecular weight of 1,000 or less on a carrier using a solvent; a step of removing the solvent; and a step of removing the compound having the asymmetric structure of the molecular weight of 1,000 or less by washing.
[0017] Further, according to the present invention, there is provided a method of producing a novel separating agent for separating enantiomeric isomers, characterized by including: a step of supporting an optically active polymer compound on a carrier using a solvent; a step of additionally supporting a compound having an asymmetric structure of a molecular weight of 1,000 or less on the carrier; and a step of removing the solvent.
[0018] Further, according to the present invention, there is provided a method of producing a novel separating agent for separating enantiomeric isomers, characterized by including: a step of supporting an optically active polymer compound on a carrier using a solvent; a step of additionally supporting a compound having an asymmetric structure of a molecular weight of 1, 000 or less on the carrier; a step of removing the solvent; and a step of removing the compound having the asymmetric structure of the molecular weight of 1,000 or less by washing.
[0019] Further, according to the present invention, there is provided a separating agent for separating enantiomeric isomers, including an optically active polymer compound supported on a carrier, from which a compound having an asymmetric structure of a molecular weight of 1,000 or less added as a production raw material and supported is removed.
[0020] Further, according to the present invention, there is provided an immobilizing phase for a chromatography using the separating agent for separating enantiomeric isomers or an immobilizing phase for a continuous liquid preparative chromatography using the separating agent for separating enantiomeric isomers or a separation method for enantiomeric isomers using the separating agent for separating enantiomeric isomers. Best Mode for carrying out the Invention
[0021] A method of producing a novel separating agent for separating enantiomeric isomers of the present invention is described.
[0022] The production method of the present invention includes a step of adding a compound having an asymmetric structure of a molecular weight of 1,000 or less in supporting an optically active polymer compound on a carrier, and a production method including the following steps can be exemplified as the production method including the above step.
[0023] Firstly, in a first step, an optically active polymer compound and a compound having an asymmetric structure of a molecular weight of 1, 000 or less are supported on a carrier using a solvent. Here, in the present invention, the optically active polymer compound is directly supported on the carrier. However, the compound having the asymmetric structure of the molecular weight of 1,000 or less is not directly supported on the carrier, but is indirectly supported on the carrier by physically or chemically bonding it to the optically active polymer compound.
[0024] In this step, the following methods can be applied:
(1) a method of preparing a solvent solution of an optically active polymer compound and the compound having the asymmetric structure of the molecular weight of 1,000 or less, and bringing the solution and the carrier in contact with each other by a method of immersing the carrier in the solution, a method of applying the solution to the carrier, or the like, thereby supporting it on the carrier; and
(2) a method of preparing the solvent solution of the optically active polymer compound, contact-supporting the solution on the carrier by a method of immersing the carrier in the solution, and dissolving the compound having the asymmetric structure of the molecular weight of 1, 000 or less in the solution, or after preparation of the solvent solution, adding the solvent solution of the compound having the asymmetric structure of the molecular weight of 1,000 or less to the solution, thereby supporting it on the carrier.
[0025] The term "supporting" used herein means that an optically active polymer compound and a compound having an asymmetric structure of a molecular weight of 1,000 or less are fixed to the carrier. This fixation is performed by physical adsorption and/or chemical bonding between the optically active polymer compound and a compound having an asymmetric structure of a molecular weight of 1,000 or less, and a carrier.
[0026] The physical adsorption means that the compound is adsorbed on a surface of the carrier and/or inside fine pores of the carrier.
[0027] The chemical bonding includes bonding between a carrier and an optically active polymer compound, bonding between a part of the optically active polymer compound physically adsorbed on the carrier, bonding between remaining optically active polymer compound and the compound having the asymmetric structure of the molecular weight of 1,000 or less, and chemical bonding between the carrier and the optically active polymer compound by reaction with a crosslinking agent, reaction with a radical generator or light irradiation (irradiation with radiation such as γ-rays, or irradiation with electromagnetic waves such as microwave).
[0028] When chemically bonding the carrier and the optically active polymer compound, it is desirable to perform chemical bonding before or after a step of removing the compound having the asymmetric structure of the molecular weight of 1,000 or less as a post-step.
[0029] Examples of the optically active polymer compound used in the present invention include polymers or copolymers of (meth) acrylates or (meth) acrylamides that do not have optically active substituents, (meth)acrylates or (meth)acrylamides having the optically active substituents, styrene, acetylene or the like, polysaccharides or their derivatives, peptides, and proteins.
[0030] Of those, the polymer compounds having the asymmetry identification ability to a compound to be separated are preferable. In particular, the polymers or copolymers of (meth)acrylates or (meth)acrylamides, polysaccharides and their derivatives, and proteins that are known to have the asymmetry identification ability are preferable, polymers or copolymers of (meth)acrylamides or (meth)acrylates, polysaccharides and their derivatives having optically active substituents at side chains are more preferable, and polysaccharide derivatives are most preferable.
[0031] Note that, the (meth) acrylates used in this specification mean acrylates and methacrylates, and the (meth) acrylamides used in this specification mean acrylamides and methacrylamides.
[0032] As a polysaccharide, any synthetic polysaccharide, any natural polysaccharide, and any modified natural polysaccharide may be used so long as they have an optical activity. Those which have a high regularity in the binding form are more desired.
[0033] There are exemplified β-1,4-glucan (cellulose), α-1,4-glucan (amylose, amylopectin), α-1,6-glucan (dextran), β-1,6-glucan (busturan), β-1,3-glucan (for example, cardran, schizophyllan, etc.), α-1,3-glucan, β-1,2-glucan (Crown Gall polysaccharide), β-1,4-galactan, β-1,4-mannan, α-1,6-mannan, β-1,2-fructan (inulin), β-2,6-fructan (levan), β-1,4-xylan, β-1,3-xylan, β-1,4-chitosan, α-1,4-N-acetylchitosan (chitin), pullulan, agarose, alginic acid, and the like. Also, the polysaccharide includes starch containing amylose.
[0034] Among those, cellulose, amylose, β-1,4-xylan, β-1,4-chitosan, chitin, β-1,4-mannan, inulin, and cardran, which are readily available as the polysaccharide having high purity, are preferred. Cellulose and amylose are particularly preferred.
[0035] These polysaccharides have a number-average degree of polymerization (an average number of pyranose rings or furanose rings contained in one molecule) of at least 5, preferably at least 10, or preferably 1,000 or less in view of ease of handling, though there is no particular limitation in the upper limit thereof.
[0036] The polysaccharide derivative is a compound being combined with a compound having a functional group being reactive with part or all of the hydroxyl groups of the polysaccharide through an ester bond, an urethane bond or an ether bond.
[0037] The compound having a functional group capable of reacting with hydroxyl group can be any compound so long as it is a compound having leaving groups such as substituted or unsubstituted aromatic, aliphatic or alicyclic carboxylic acids, acid halides, acid anhydrides, carboxylic acid derivatives such as acid ester, substituted or unsubstituted aromatic, aliphatic or alicyclic isocyanic acid derivatives, alcohols, and other compounds. The compound may have or may not have optically active groups.
[0038] Preferable polysaccharide derivatives are polysaccharide ester derivatives and carbamate derivatives, and polysaccharide ester derivatives and carbamate derivatives having 0.1 or more, per glucose unit, of ester bond or urethane bond are particularly preferable.
[0039] The amount of the optically active polymer compound used is such an amount that the amount of the compound supported on a carrier with respect to a carrier mass preferably corresponds to 1 to 100 mass%, more preferably 5 to 60 mass%, and most preferably 10 to 40 mass%.
[0040] The compound having the asymmetric structure of the molecular weight of 1,000 or less used in the present invention includes the following (I) and (II).
(I) A compound to be separated in the case of being used as a separating agent for
separating enantiomeric isomers, or its similarly structured compound. The "similarly
structured compound" used herein is a compound in which a functional group is similar
to that of the compound to be separated, the number of methylene chains increases
or decreases, the number of substituents increases or decreases, a position of the
functional group differs, and a kind of functional group differs from the compound
to be separated and which accordingly has such a structure that a molecular size is,
for example, larger by about 1 to 5 carbon atoms or smaller by about 1 to 5 carbon
atoms. Examples of the similarly structured compound, with respect to 1-phenyl-2-propanol,
include 1-phenylethanol, 2-phenyl-2-propanol, 1-phenyl-2-butanol, 2-phenyl-2-butanol,
3-phenyl-2-butanol, 1-substituted phenyl-2-propanol, 1-(1-naphthyl)-2-propanol, 1-(2-pyridyl)-2-propanol,
and 1-cyclohexyl-2-propanol. Example of the similarly structured compound, with respect
to 1-(1-naphthyl)-ethanol, include 1-(9-anthryl)-ethanol.
Besides, the "similarly structured compound" includes compounds in which environmental
conditions around the asymmetric carbon are similar.
(II) A compound to be separated in the case of being used as a separating agent for separating enantiomeric isomers, or a compound other than a similarly structured compound thereof, preferably a compound having a cyclic structure of a molecular weight of 40 to 1,000, preferably 60 to 600.
[0041] Preferable compounds (I) and (II) are compounds having polar functional group such as a heteroatomic group, e.g., a hydroxyl group, a carbonyl group, an amino group or a carboxyl group, compounds having a functional group related to π-electron interaction, such as benzene ring, racemi form (±), and optically active substance (+) or (-) are preferable.
[0042] The compound (I) has a molecular weight of 40 ormore, preferably 60 to 1,000, and more preferably 100 to 500.
[0043] The compound having the asymmetric structure of the molecular weight of 1,000 or less is used in an amount of preferably 0.01 to 1, 000 mass%, more preferably 0.01 to 200 mass%, and most preferably 0.1 to 30 mass%, based on the mass of the optically active polymer compound supported on a carrier.
[0044] The carrier used in the present invention includes organic porous carriers and inorganic porous carriers. The inorganic porous carriers are preferable. Suitable examples of the organic porous carrier include polymer substances including polystyrenes, polyacrylamides, polyacrylates, or the like. Suitable examples of the inorganic porous carrier include silica, alumina, magnesia, glass, kaolin, titanium oxide, silicates and hydroxyapatites.
[0045] Silica gel is a particularly preferable carrier. The silica gel has a particle diameter of 0.1 µm to 10 mm, preferably 1 µm to 300 µm, and most preferably 1 to 100 µm, and an average pore diameter of 10 Å to 100 µm, and preferably 50 to 50, 000 Å. Surface treatment may be applied to the surface of silica gel in order to eliminate effects of residual silanol, but there is no problem even though the surface treatment is not applied to the surface.
[0046] The solvent used in the present invention may be any generally-used organic solvent so long as it can dissolve the optically active polymer compound and the compound having the asymmetric structure of the molecular weight of 1,000 or less.
[0047] Examples of the solvent include ketone solvents such as acetone, ethyl methyl ketone or acetophenone; ester solvents such as ethyl acetate, methyl acetate, propyl acetate, methyl propionate or phenyl acetate; ether solvents such as tetrahydrofuran, 1,4-dioxane, diethyl ether, tert-butyl methyl ether or anisole; amide solvents such as N,N-dimethylformamide; imide solvents such as N,N-dimethylimidazolidinone; halogen solvents such as chloroform, methylene chloride, carbon tetrachloride, 1,2-dichloroethane or pentafluoroethanol; hydrocarbon solvents such as pentane, petroleum ether, hexane, heptane, octane, benzene, toluene, xylene or mesitylene; alcohol solvents such as methanol, ethanol, propanol or butanol; acid solvents such as acetic acid, trifluoroacetic acid or formic acid; phenol solvent such as phenol or catechol; and amine solvents such as diethylamine, triethylamine, pyridine or aniline.
[0048] In preparing the optically active polymer compound and the compound having the asymmetric structure of the molecular weight of 1,000 or less using such a solvent, a solution concentration is not particularly limited, and is determined considering ease of contact-supporting treatment with the carrier, and removal treatment of a solvent in a post-step.
[0049] A solvent used to support the optically active polymer compound and the compound having the asymmetric structure of the molecular weight of 1,000 or less on the carrier is removed in the next step.
[0050] In the solvent removal treatment in this step, the optically active polymer compound is still directly supported on the carrier, and the compound having the asymmetric structure of the molecular weight of 1,000 or less maintains the state of being indirectly supported on the carrier.
[0051] In the next step, the optically active polymer compound and the compound having the asymmetric structure of the molecular weight of 1,000 or less are washed in the state where they are supported on the carrier, thereby removing the compound having the asymmetric structure of the molecular weight of 1,000 or less.
[0052] The washing step can adopt, for example, a method of refluxing under any temperature of 0°C to a reflux temperature using acetonitrile, alcohol, hexane, a mixed solvent of hexane and alcohol, or the like.
[0053] The amount of solvent used in the case of adopting the reflux method is about 3 to 50 times the mass of the carrier having supported thereon the optically active polymer compound and the compound having the asymmetric structure of the molecular weight of 1,000 or less.
[0054] A residual amount of the compound having the asymmetric structure of the molecular weight of 1,000 or less after the washing treatment of this step is, when finally formed into a separating agent for separating enantiomeric isomers, preferably 10 mass% or less, more preferably 2 mass% or less, and most preferably 0.5 mass% or less, in the separating agent. The compound having the asymmetric structure of the molecular weight of 1,000 or less may be contained as an impurity if the content is less than the above value.
[0055] Further, the compound having the asymmetric structure of the molecular weight of 1,000 or less after the washing treatment of this step can be substantially removed as well.
[0056] A separating agent for separating enantiomeric isomers obtained according to the producing method of the present invention includes an optically active polymer compound supported on a carrier, further, from which a compound having an asymmetric structure of a molecular weight of 1,000 or less added as a production raw material and supported is removed.
[0057] In the separating agent for separating enantiomeric isomers obtained according to the producing method of the present invention, the compound having the asymmetric structure of the molecular weight of 1,000 or less is the compound of the item (I) or (II), and when the enantiomeric isomers are separated using the separating agent for separating the enantiomeric isomers, a separation performance based on a separation coefficient (α) obtained by the following equation:
[where, the dead time is set as elution time of tri-tert-butylbenzene] is desirably shown by the following equation (A) or equation (B):
(A) α1/α2≥1.05 (provided α2=1.00)
(B) α1/α2≥1.05 (provided α2>1.00)
where α1: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained by adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in a production step, andα2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
[0058] The equation (A) shows α2=1.00, that is, the fact that separation performance of a compound that involves absolutely no separation in the case of using a separating agent for separating enantiomeric isomers obtained without adding a compound having an asymmetric structure of a molecular weight of 1,000 or less in the production step is improved by 5% or more in the case of using the separating agent for separating enantiomeric isomers of the present invention.
[0059] The equation (B) shows α2>1.00, that is, the fact that separation performance in the case of using a separating agent for separating enantiomeric isomers obtained without adding a compound having an asymmetric structure of a molecular weight of 1,000 or less in the production step is improved by 5% or more in the case of using the separating agent for separating enantiomeric isomers of the present invention. In the present invention, the separation performance defined by the equation (B) is improved by preferably 10% or more, more preferably 15% or more, and most preferably 20% or more.
[0060] The separating agent for separating enantiomeric isomers of the present invention can be used as an immobilizing phase for chromatography such as gas chromatography, liquid chromatography, thin layer chromatography, supercritical chromatography or capillary electrophoresis. In particular, it is preferably used as the chiral immobilizing phase for liquid chromatography. It can also be suitably used as an immobilizing phase for continuous liquid preparative chromatography represented by a simulated moving bed chromatography. The enantiomeric isomers can be separated with good efficiency using such a separating agent of the present invention. Industrial Applicability
[0061] The present invention can greatly improve the separation performance of a separating agent for separating enantiomeric isomers, and particularly, such a separating agent is suitable as an immobilizing phase for chromatography and an immobilizing phase for continuous liquid preparative chromatography.
Examples
[0062] The present invention is described in detail based on examples, but the present invention is not limited to those examples.
[0063] A representation of the compounds described hereinafter is based on any compound selected from the following compounds 1 to 16. With respect to a compound having an asymmetric structure of a molecular weight of 1,000 or less and a compound to be separated, representationof steric configuration (S,R), optical activity (D, L) and racemi form is combined with each compound number. For example, when the compound 1 has an S configuration, it is expressed as S-1, and when the compound 1 is a racemi form, it is expressed as racemi form-1.
Example 1
(1) Surface treatment of carrier (silica gel)
[0064] Porous silica gel (particle diameter: 7 µm, micropore: 1,000 Å) was reacted with 3-aminopropyltriethoxysilane using any conventional method to perform aminopropylsilane treatment.
(2) Synthesis of optically active polymer compound
[0065] 15.0 g of lithium chloride in an absolute dry statewas dissolved in 150 ml of N,N-dimethylacetamide (DMAc) to prepare a DMAc/LiCl solution.
[0066] In a nitrogen atmosphere, 150 ml of the above DMAc/LiCl solution and 150 ml of pyridine were added to 10.0 g of cellulose, and the resulting mixture was immersed in an oil bath at 100°C and stirred for 24 hours. Thereafter, 50 g of 4-methylbenzoyl chloride was added to the mixture to conduct reaction at 100°C for 16 hours.
[0067] The reaction liquid was added dropwise to 2 L of methanol, followed by reprecipitation and centrifugal separation, thereby obtaining the objective cellulose tris(4-methylbenzoate) represented by the following formula.
(3) Preparation of separating agent for separating enantiomeric isomers
[0068] 0.8 g of cellulose tris (4-methylbenzoate) obtainedin (2) above and 506.0 mg of a compound (S-1) (2-fold molar equivalent to glucose unit of cellulose tris(4-methylbenzoate)) were dissolved in methylene chloride to prepare a dope. This dope was applied to 3.2 g of silica gel obtained in (1) above. After the application, methylene chloride was distilled off to obtain the objective separating agent for separating enantiomeric isomers. This separating agent was added to a mixed solvent of n-hexane/2-propanol, and the resulting mixture was well stirred, and filtered. The filtrate was condensed to recover 495.6 mg of (S-1).
(4) Preparation of packed column for HPLC
[0069] A stainless steel-made column having a length of 25 cm and an inner diameter of 0.46 cm was packed with the separating agent for separating enantiomeric isomers obtained in (3) above by a slurry packing method using a mixed solvent of n-hexane/2 -propanol to obtain a separation column for enantiomeric isomers.
[0070] S-1 residual amount in the separating agent for separating enantiomeric isomers: 506.0-495.6=10.4 mg
[0072] S-1 residual amount in the separating agent of the separation column for enantiomeric isomers:
Example 2
(1) Surface treatment of carrier (silica gel)
[0073] The silica gel treated with aminopropylsilane was obtained in the same manner as in Example 1.
(2) Synthesis of optically active polymer compound
[0074] In a nitrogen atmosphere, 10.0 g of amylose was added to 300 ml of pyridine. The resulting mixture was immersed in an oil bath at 100°C, and 50 g of (S)-phenylethylisocyanate was added thereto to conduct reaction at 100°C for 48 hours. The reaction liquid was added dropwise to 2 L of methanol, followed by reprecipitation and centrifugal separation, thereby obtaining the objective amylose tris[(S)-phenylethylcarbamate] represented by the following equation.
(3) Preparation of separating agent for separating enantiomeric isomers
[0075] 0.8 g of amylose trisp[(S)-phenylethylcarbamate] obtained in (2) above and 435.65 mg of (S-1) (2-foldmolar equivalent to a glucose unit of amylose tris [(S)-phenylethylcarbamate]) were dissolved in THF to prepare a dope. This dope was applied to 3.2 g of silica gel obtained in (1) above. After the application, THF was distilled off to obtain the objective separating agent for separating enantiomeric isomers. This separating agent was added to a mixed solvent of n-hexane/2-propanol, and the resulting mixture was well stirred, and filtered. The filtrate was condensed to recover 416.0 mg of (S-1).
(4) Preparation of packed column for HPLC
[0076] A separation column for enantiomeric isomers was obtained in the same manner as in Example 1.
Example 3
(1) Surface treatment of carrier (silica gel)
[0080] The silica gel treated with aminopropylsilane was obtained in the same manner as in Example 1.
(2) Synthesis of optically active polymer compound
[0081] The objective cellulose tris(3,5-dimethylphenylcarbamate) represented by the following formula was obtained in the same manner as in Example 2.
(3) Preparation of separating agent for separating enantiomeric isomers
[0082] 0.8 g of cellulose tris(3,5-dimethylphenylcarbamate) obtained in (2) above and 435. 9 mg of (S-1) (2-fold molar equivalent to a glucose unit of cellulose tris(3,5-dimethylphenylcarbamate) were dissolved in acetone to prepare a dope. This dope was applied to 3.2 g of silica gel obtained in (1) above. After the application, acetone was distilled off to obtain the objective separating agent for separating enantiomeric isomers. This separating agent was added to a mixed solvent of n-hexane/2-propanol, and the resulting mixture was well stirred, and filtered. The filtrate was condensed to recover 420.6 mg of (S-1).
(4) Preparation of packed column for HPLC
[0083] A separation column for enantiomeric isomers was obtained in the same manner as in Example 1.
Example 4
(1) Surface treatment of carrier (silica gel)
[0087] The silica gel treated with aminopropylsilane was obtained in the same manner as in Example 1.
(2) Synthesis of optically active polymer compound
[0088] The objective amylose tris(3,5-dimethylphenylcarbamate) represented by the following formula was obtained in the same manner as in Example 2.
(3) Preparation of separating agent for separating enantiomeric isomers
[0089] 0.8 g of amylose tris(3,5-dimethylphenylcarbamate) obtained in (2) above and 436.3 mg of (S-1) were dissolved in ethyl acetate to prepare a dope. This dope was applied to 3.2 g of silica gel obtained in (1) above. After the application, ethyl acetate was distilled off to obtain the obj ective separating agent for separating enantiomeric isomers. This separating agent was added to a mixed solvent of n-hexane/2-propanol, and the resulting mixture was well stirred, and filtered. The filtrate was condensed to recover 427.6 mg of (S-1).
(4) Preparation of packed column for HPLC
[0090] A separation column for enantiomeric isomers was obtained in the same manner as in Example 1.
Examples 5 to 24
[0094] Using a compound having an asymmetric structure of a molecular weight of 1,000 or less shown in Table 1, the objective separating agent for separating enantiomeric isomers was obtained by the same production method of each of Examples 1 to 3, and thereafter a separation column for enantiomeric isomers was obtained.
Table 1
| Example | Production method (selected from Examples 1 to 3) | Kind of compound having asymmetric structure of molecular weight of 1,000 or less |
| 5 | 2 | S-3 |
| 6 | 2 | D-6 |
| 7 | 2 | L-6 |
| 8 | 2 | S-7 |
| 9 | 2 | R-8 |
| 10 | 2 | S-8 |
| 11 | 2 | S-9 |
| 12 | 3 | L-6 |
| 13 | 2 | S-11 |
| 14 | 2 | R-9 |
| 15 | 1 | R-3 |
| 16 | 1 | S-3 |
| 17 | 1 | D-6 |
| 18 | 1 | R-8 |
| 19 | 1 | Racemi form-13 |
| 20 | 1 | Racemi form-5 |
| 21 | 1 | Racemi form-2 |
| 22 | 1 | Racemi form-14 |
| 23 | 1 | Racemi form-15 |
| 24 | 1 | Racemi form-16 |
Comparative Example 1
[0095] The objective separating agent for separating enantiomeric isomers was obtained in the same manner as in Example 1, and thereafter a separation column for enantiomeric isomers was obtained. However, (S-1) was not added.
Comparative Example 2
[0096] The objective separating agent for separating enantiomeric isomers was obtained in the same manner as in Example 2, and thereafter a separation column for enantiomeric isomers was obtained. However, (S-1) was not added.
Comparative Example 3
[0097] The objective separating agent for separating enantiomeric isomers was obtained in the same manner as in Example 3, and thereafter a separation column for enantiomeric isomers was obtained. However, (S-1) was not added.
Comparative Example 4
[0098] The objective separating agent for separating enantiomeric isomers was obtained in the same manner as in Example 4, and thereafter a separation column for enantiomeric isomers was obtained. However, (S-1) was not added.
Application Examples 1 to 45
[0099] Using the separation columns for enantiomeric isomers obtained in Examples 1 to 24 and Comparative Examples 1 to 4, α values were measured with a liquid chromatography (liquid chromatograph, manufactured by JASCO Co.). The measurement conditions include moving phase: n-hexane/2-propanol=90/10, flow rate: 1.0 ml/min, temperature: 25°C, and detection wavelength: 254 nm. The results are shown in Tables 2 to 4.
[0100] The judgement in the tables is judgement of identity, similarity or non-similarity, and the details thereof are described after the tables. An increase rate (%) of α value in the tables was obtained by the following equation: α1-α2/α1×100. Here, expressed by α=1.00 is the state where asymmetry identification was not conducted at all and only one peak was observed.
[0101] Application Examples 4 and 18: The compound having the asymmetric structure and the compound to be separated each have an asymmetric carbon atom at the root of a phenyl group. Further, adjacent carbon atoms (β-position) of the asymmetric carbon atoms each have a carbonyl group, and the environment around the asymmetric carbon atoms is similar.
[0102] Application Examples 5, 8, 16 and 19: The compound having the asymmetric structure and the compound to be separated each have an asymmetric carbon atom at the root of a hydroxyl group. Further, the environment around the asymmetric carbon atom is similar.
[0103] Application Examples 6, 7 and 9: The compound having the asymmetric structure and the compound to be separated each have an asymmetric carbon atom at the root of a hydroxyl group. Further, adjacent carbon atoms (α-position) of the asymmetric carbon atoms are each carbonyl groups, and thus, the environment around the asymmetric carbon atoms is similar.
[0104] Application Examples 10, 12 and 14: The compound having the asymmetric structure and the compound to be separated each have an asymmetric carbon atom adjacent to an oxygen atom (α-position). Further, adjacent carbon atoms (β-position) of the asymmetric carbon atoms each have a carbonyl group, and the environment around the asymmetric carbon atoms is similar.
[0105] Application Example 11: The compound having the asymmetric structure and the compound to be separated each have an asymmetric carbon atom adjacent to a carbon atom and have a carbonyl group in a molecule. Thus, the environment around the asymmetric carbon atoms is similar.
[0106] Application Example 13: The compound having the asymmetric structure and the compound to be separated each have an asymmetric carbon atom at the root of a trihalogenomethyl substituent. Further, the environment around the asymmetric carbon atom is similar.
[0107] Application Examples 15 and 17: The compound having he asymmetric structure and the compound to be separated each have an asymmetric carbon atom adjacent to an oxygen atom (α-position) and have a carbonyl group in the vicinity of the asymmetric carbon atoms, and the environment around the asymmetric carbon atoms is similar.
[0108] Application Example 40: The compound having the asymmetric structure and the compound to be separated each have an asymmetric carbon atom at the root of a hydroxyl group, and the structure of molecule as a whole includes a condensed ring similar to a naphthyl group and an anthranil group. Therefore, the environment around the asymmetric carbon atom is similar.
1. A method of producing a novel separating agent for separating enantiomeric isomers,
characterized by comprising adding a compound having an asymmetric structure of a molecular weight
of 1,000 or less in supporting an optically active polymer compound on a carrier.
2. A method of producing a novel separating agent for separating enantiomeric isomers,
characterized by comprising: a step of supporting an optically active polymer compound and a compound
having an asymmetric structure of a molecular weight of 1,000 or less on a carrier
using a solvent; and a step of removing the solvent.
3. A method of producing a novel separating agent for separating enantiomeric isomers,
characterized by comprising: a step of supporting an optically active polymer compound and a compound
having an asymmetric structure of a molecular weight of 1,000 or less on a carrier
using a solvent; a step of removing the solvent; and a step of removing the compound
having the asymmetric structure of the molecular weight of 1,000 or less by washing.
4. A method of producing a novel separating agent for separating enantiomeric isomers,
characterized by comprising: a step of supporting an optically active polymer compound on a carrier
using a solvent; a step of additionally supporting a compound having an asymmetric
structure of a molecular weight of 1,000 or less on the carrier; and a step of removing
the solvent.
5. A method of producing a novel separating agent for separating enantiomeric isomers,
characterized by comprising: a step of supporting an optically active polymer compound on a carrier
using a solvent; a step of additionally supporting a compound having an asymmetric
structure of a molecular weight of 1,000 or less on the carrier; a step of removing
the solvent; and a step of removing the compound having the asymmetric structure of
the molecular weight of 1,000 or less by washing.
6. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 5, wherein supporting the optically active polymer
compound on the carrier is conducted by physical adsorption.
7. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 5, wherein supporting the optically active polymer
compound on the carrier is conducted by chemical bonding.
8. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in claim 7, wherein the carrier and the optically active polymer compound
are chemically bonded before or after the removal of the compound having the asymmetric
structure of the molecular weight of 1,000 or less.
9. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in claim 7, wherein the carrier and the optically active polymer compound
are chemically bonded by a reaction with a crosslinking agent, a reaction with a radical
generator or irradiation with light before or after the removal of the compound having
the asymmetric structure of the molecular weight of 1,000 or less.
10. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 9, wherein the optically active polymer compound
has an asymmetry identification ability to a compound to be separated when used as
the separating agent for separating the enantiomeric isomers.
11. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 10, wherein the optically active polymer compound
is a polysaccharide derivative.
12. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 11, wherein the optically active polymer compound
is a polysaccharide ester derivative or carbamate derivative.
13. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 12, wherein the optically active polymer compound
is a cellulose derivative or an amylose derivative.
14. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 10, wherein the optically active polymer compound
is a polymer or a copolymer of acrylamides, methacrylamides, acrylates or methacrylates
having an optically active substituents on a side chain.
15. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 14, wherein an amount of the optically active
polymer compound supported on the carrier is 1 to 100 mass% to a mass of the carrier.
16. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 15, wherein the compound having the asymmetric
structure of the molecular weight of 1,000 or less is a compound tobe separated or
a similarly structured compound thereof when used as the separating agent for separating
the enantiomeric isomers.
17. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 15, wherein the compound having the asymmetric
structure of the molecular weight of 1,000 or less is not a compound to be separated
or a similarly structured compound thereof when used as the separating agent for separating
the enantiomeric isomers.
18. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in claim 17, wherein the compound having the asymmetric structure of the
molecular weight of 1,000 or less is not the compound to be separated or the similarly
structured compound thereof when used as the separating agent for separating the enantiomeric
isomers, and is a compound having a cyclic structure of a molecular weight of 40 to
1000.
19. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 18, wherein the compound having the asymmetric
structure of the molecular weight of 1,000 or less is a racemi form and/or an optically
active substance.
20. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 19, wherein the amount of the compound having
the asymmetric structure of the molecular weight of 1,000 or less supported on the
carrier is 0.01 to 1,000 mass% to a mass of the optically active polymer compound.
21. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 20, wherein a residual amount of the compound
having the asymmetric structure of the molecular weight of 1,000 or less is 10 mass%
or less in the separating agent for separating the enantiomeric isomers.
22. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed any one of claims 1 or 20, wherein the compound having the asymmetric structure
of the molecular weight of 1,000 or less is substantially removed.
23. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 22, wherein the compound having the asymmetric
structure of the molecular weight of 1,000 or less is the compound to be separated
or the similarly structured compound thereof when used as the separating agent for
separating the enantiomeric isomers, and when the enantiomeric isomers are separated
using the obtained separating agent for separating the enantiomeric isomers, a separation
performance based on a separation coefficient (α) obtained by the following equation:
is represented by the following equation (A) or equation (B):
α2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
is represented by the following equation (A) or equation (B):
(A) α1/α2≥1.05 (provided α2=1.00)
(B) α1/α2≥1.05 (provided α2>1.00)
where α1: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained by adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in a production step, andα2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
24. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in any one of claims 1 to 22,
wherein the compound having the asymmetric structure of the molecular weight of 1,000 or less is not the compound to be separated or the similarly structured compound thereof when used as the separating agent for separating the enantiomeric isomers, and when the enantiomeric isomers are separated using the obtained separating agent for separating the enantiomeric isomers, a separation performance based on a separation coefficient (α) obtained by the following equation:
is represented by the following equation (A) or equation (B):
α2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
wherein the compound having the asymmetric structure of the molecular weight of 1,000 or less is not the compound to be separated or the similarly structured compound thereof when used as the separating agent for separating the enantiomeric isomers, and when the enantiomeric isomers are separated using the obtained separating agent for separating the enantiomeric isomers, a separation performance based on a separation coefficient (α) obtained by the following equation:
is represented by the following equation (A) or equation (B):
(A) α1/α2≥1.05 (provided α2=1.00)
(B) α1/α2≥1.05 (provided α2>1.00)
where α1: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained by adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in a production step, andα2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
25. The method of producing a novel separating agent for separating enantiomeric isomers
as claimed in claim 24, wherein the compound having the asymmetric structure of the
molecular weight of 1,000 or less is not the compound to be separated or the similarly
structured compound thereof when used as the separating agent for separating the enantiomeric
isomers, and is a compound having a cyclic structure of a molecular weight of 40 to
1000.
26. A separating agent for separating enantiomeric isomers, comprising an optically active
polymer compound supported on a carrier, from which a compound having an asymmetric
structure of a molecular weight of 1,000 or less added as a production raw material
and supported is removed.
27. The separating agent for separating enantiomeric isomers as claimed in claim 26, wherein
a residual amount of the compound having the asymmetric structure of the molecular
weight of 1,000 or less is 10 mass% or less in the separating agent for separating
the enantiomeric isomers.
28. The novel separating agent for separating enantiomeric isomers as claimed in claim
26 or 27, wherein the compound having the asymmetric structure of the molecular weight
of 1,000 or less is substantially removed.
29. The novel separating agent for separating enantiomeric isomers as claimed in any one
of claims 26 to 28, wherein supporting the optically active polymer compound on the
carrier is conducted by physical adsorption.
30. The novel separating agent for separating enantiomeric isomers as claimed in any one
of claims 26 to 28, wherein supporting the optically active polymer compound on the
carrier is conducted by chemical bonding.
31. The novel separating agent for separating enantiomeric isomers as claimed in claim
30, wherein the carrier and the optically active polymer compound are chemically bonded
before or after the removal of the compound having the asymmetric structure of the
molecular weight of 1,000 or less.
32. The novel separating agent for separating enantiomeric isomers as claimed in claim
30, wherein the carrier and the optically active polymer compound are chemically bonded
by a reaction with a crosslinking agent, a reaction with a radical generator or irradiation
with light before or after the removal of the compound having the asymmetric structure
of the molecular weight of 1,000 or less.
33. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 32, wherein the optically active polymer compound has an asymmetry identification
ability to a compound to be separated when used as the separating agent for separating
the enantiomeric isomers.
34. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 33, wherein the optically active polymer compound is a polysaccharide
derivative.
35. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 34, wherein the optically active polymer compound is a polysaccharide
ester derivative or carbamate derivative.
36. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 35, wherein the optically active polymer compound is a cellulose derivative
or an amylose derivative.
37. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 33, wherein the optically active polymer compound is a polymer or a copolymer
of acrylamides, methacrylamides, acrylates or methacrylates having an optically active
substituent on a side chain.
38. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 37, wherein an amount of the optically active polymer compound supported
on the carrier is 1 to 100 mass% to a mass of the carrier.
39. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 38, wherein the compound having the asymmetric structure of the molecular
weight of 1,000 or less is a compound to be separated or a similarly structured compound
thereof when used as the separating agent for separating the enantiomeric isomers.
40. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 38, wherein the compound having the asymmetric structure of the molecular
weight of 1,000 or less is not the compound to be separated or a similarly structured
compound thereof when used as the separating agent for separating the enantiomeric
isomers.
41. The novel separating agent for separating enantiomeric isomers as claimed in claim
40, wherein the compound having the asymmetric structure of the molecular weight of
1,000 or less is not the compound to be separated or a similarly structured compound
thereof when used as the separating agent for separating the enantiomeric isomers,
and is a compound having a cyclic structure of a molecular weight of 40 to 1000.
42. The novel separating agent for separating enantiomeric isomers as claimed in any one
of claims 26 to 41, wherein the compound having the asymmetric structure of the molecular
weight of 1,000 or less is a racemi form and/or an optically active substance.
43. The separating agent for separating enantiomeric isomers as claimed in any one of
claims 26 to 42, wherein the amount of the compound having the asymmetric structure
of the molecular weight of 1,000 or less supported on the carrier is 0.01 to 1,000
mass% to a mass of the optically active polymer compound.
44. The novel separating agent for separating enantiomeric isomers as claimed in any one
of claims 26 to 43,
wherein the compound having the asymmetric structure of the molecular weight of 1,000 or less is a compound to be separated or a similarly structured compound thereof when used as a separating agent for separating enantiomeric isomers, and when the enantiomeric isomers are separated using the separating agent for separating the enantiomeric isomers, a separation performance based on a separation coefficient (α) obtained by the following equation:
is represented by the following equation (A) or equation (B):
α2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
wherein the compound having the asymmetric structure of the molecular weight of 1,000 or less is a compound to be separated or a similarly structured compound thereof when used as a separating agent for separating enantiomeric isomers, and when the enantiomeric isomers are separated using the separating agent for separating the enantiomeric isomers, a separation performance based on a separation coefficient (α) obtained by the following equation:
is represented by the following equation (A) or equation (B):
(A) α1/α2≥1.05 (provided α2=1.00)
(B) α1/α2≥1.05 (provided α2>1.00)
where α1: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained by adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in a production step, andα2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
45. The novel separating agent for separating enantiomeric isomers as claimed in any one
of claims 26 to 43,
wherein the compound having the asymmetric structure of the molecular weight of 1,000 or less is not the compound to be separated or the similarly structured compound thereof when used as the separating agent for separating the enantiomeric isomers, and when the enantiomeric isomers are separated using the separating agent for separating the enantiomeric isomers, a separation performance based on a separation coefficient (α) obtained by the following equation:
is represented by the following equation (A) or equation (B):
α2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
wherein the compound having the asymmetric structure of the molecular weight of 1,000 or less is not the compound to be separated or the similarly structured compound thereof when used as the separating agent for separating the enantiomeric isomers, and when the enantiomeric isomers are separated using the separating agent for separating the enantiomeric isomers, a separation performance based on a separation coefficient (α) obtained by the following equation:
is represented by the following equation (A) or equation (B):
(A) α1/α2≥1.05 (provided α2=1.00)
(B) α1/α2≥1.05 (provided α2>1.00)
where α1: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained by adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in a production step, andα2: a separation coefficient of the separating agent for separating the enantiomeric isomers obtained without adding the compound having the asymmetric structure of the molecular weight of 1,000 or less in the production step, in which α2=1.00 means that the enantiomeric isomers are not separated al all, and α2>1.00 means that the enantiomeric isomers are separated.
46. The separating agent for separating enantiomeric isomers as claimed in claim 45, wherein
the compound having the asymmetric structure of the molecular weight of 1,000 or less
is not a compound to be separated or a similarly structured compound thereof when
used as the separating agent for separating the enantiomeric isomers, and is a compound
having a cyclic structure of a molecular weight of 40 to 1000.
47. An immobilizing phase for a chromatographyusing the separating agent for separating
enantiomeric isomers as claimed in any one of claims 26 to 46.
48. An immobilizing phase for a continuous liquid preparative chromatography using the
separating agent for separating enantiomeric isomers as claimed in any one of claims
26 to 46.
49. A separation method for enantiomeric isomers using the separating agent for separating
the enantiomeric isomers as claimed in any one of claims 26 to 46.