Biosensor for determining low density cholesterol

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EP 1 434 054 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION

(48)	Corrigendum issued on:
	27.10.2004 Bulletin 2004/44

(43)	Date of publication:
	30.06.2004 Bulletin 2004/27

(21)	Application number: 03258202.5

(22)	Date of filing: 23.12.2003

(51)	International Patent Classification (IPC)⁷: G01N 33/92, C12Q 1/60

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
	Designated Extension States:
	AL LT LV MK

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Priority:

25.12.2002 JP 2002375101

(71)	Applicant: MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD.
	Kadoma-shi, Osaka 571-8501 (JP)

(72)	Inventors:
	Watanabe, Motokazu Toyonaka-shi Osaka, 560-0025 (JP) Nakaminami, Takahiro Toyonaka-shi Osaka, 560-0033 (JP) Taniike, Yuko Osaka-shi Osaka, 542-0066 (JP) Miyashita, Mariko Nishinomiya-shi Hyogo, 663-8032 (JP) Yoshioka, Toshihiko Hirakata-shi Osaka, 573-0035 (JP)

(74)	Representative: Price, Paul Anthony King et al
	D. Young & Co., 21 New Fetter Lane London EC4A 1DA London EC4A 1DA (GB)

(54)	Biosensor for determining low density cholesterol

(57) The sensor is capable of accurately and simply measuring LDL cholesterol with only a one-time supply of a sample. A substrate of the biosensor carries cholesterol esterase, cholesterol oxidase or cholesterol dehydrogenase, a reagent for bringing a selective enzyme reaction with LDL, peroxidase, and a dye source.