| (19) |
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(11) |
EP 1 466 984 A8 |
| (12) |
CORRECTED EUROPEAN PATENT APPLICATION |
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published in accordance with Art. 158(3) EPC |
| (48) |
Corrigendum issued on: |
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26.01.2005 Bulletin 2005/04 |
| (43) |
Date of publication: |
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13.10.2004 Bulletin 2004/42 |
| (22) |
Date of filing: 18.01.2002 |
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| (51) |
International Patent Classification (IPC)7: C12P 7/42, C12P 13/00, C12N 1/20
// C12P7:42, C12R1:06, C12P13:00, C12R1:06, C12N1:20, C12R1:06 |
| (86) |
International application number: |
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PCT/JP2002/000312 |
| (87) |
International publication number: |
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WO 2003/062437 (31.07.2003 Gazette 2003/31) |
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| (84) |
Designated Contracting States: |
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AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR |
| (71) |
Applicant: NIPPON SODA CO., LTD. |
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Chiyoda-ku, Tokyo 100-8165 (JP) |
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| (72) |
Inventors: |
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- KOBAYASHI, Yoichi, c/o Nippon Soda Co., Ltd.
Odawara-shi, Kanagawa 250-0280 (JP)
- MAEDA, Kasumi, c/o Nippon Soda Co., Ltd.
Odawara-shi, Kanagawa 250-0280 (JP)
|
| (74) |
Representative: Williams, Aylsa et al |
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D Young & Co 120 Holborn
London EC1N 2DY
London EC1N 2DY (GB) |
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| |
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| (54) |
PROCESS FOR PRODUCING A-HYDROXY ACID AMMONIUM SALT |
(57) The present invention provides a method for producing a-hydroxy acid ammonium salt
that uses a specific microbial strain capable of accumulating α-hydroxy acid ammonium
salt at a high concentration while also maintaining an industrially satisfactory production
rate for a long period of time. More specifically, the present invention uses a microbial
catalyst originating in a microbial strain which, during conversion of α-hydroxynitrile
to α-hydroxy acid ammonium salt, is capable of maintaining the average production
rate of α-hydroxy acid ammonium salt at at least 100 µmol/min per g of dry microbial
cell weight for 14 days or more without adding fresh microbial catalyst, and is capable
of accumulating the α-hydroxy acid ammonium salt at 20 to 60% by weight. An example
of this microbial strain is Arthrobacter sp. strain NSSC204.