(19)
(11) EP 1 473 360 A8

(12) CORRECTED EUROPEAN PATENT APPLICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 A2)

(48) Corrigendum issued on:
28.07.2010 Bulletin 2010/30

(88) Date of publication A3:
02.02.2005 Bulletin 2005/05

(43) Date of publication:
03.11.2004 Bulletin 2004/45

(21) Application number: 04101846.6

(22) Date of filing: 17.12.1991
(51) International Patent Classification (IPC): 
C12N 5/02(1980.01)
C12N 15/00(1980.01)
C12M 3/02(1980.01)
 C12N 5/00(1980.01)
C12M 3/00(1980.01)
C12M 3/04(1980.01)
(84) Designated Contracting States:
AT BE CH DE DK ES FR GB GR IT LI LU MC NL SE

(30) Priority: 17.12.1990 US 628343
29.07.1991 US 737024
05.08.1991 US 740590

(62) Application number of the earlier application in accordance with Art. 76 EPC:
92904136.6 / 0575350

(71) Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
Ann Arbor, Michigan 48109-1248 (US)

(72) Inventors:
  • Emerson, Stephen G.
    48104, ANN ARBOR - MICHIGAN (US)
  • Clarke, Michael F.
    48103, ANN ARBOR - MICHIGAN (US)
  • Palsson, Bernhard O.
    48105, ANN ARBOR - MICHIGAN (US)
  • Schwartz, Richard M.
    48105, ANN ARBOR - MICHIGAN (US)

(74) Representative: Poulin, Gérard 
Brevalex 3, rue du Docteur Lancereaux
75008 Paris
75008 Paris (FR)

 
Remarks:
This application was filed on 29-04-2004 as a divisional application to the application mentioned under INID code 62.
 


(54) METHODS FOR THE EX-VIVO REPLICATION AND STABLE GENETIC TRANSFORMATION OF HUMAN STEM CELLS


(57) The present invention relates to methods for the manufacture of a medicament for gene therapy, to human bone marrow compositions obtained by said methods, and to uses of said compositions for the manufacture of medicaments. The method of the present invention is characterized by a specific replacement rate of the culture medium for obtaining ex vivo human stem cell division, and by the fact that said ex vivo human stem cells are genetically transformed for the purpose of said gene therapy. The replacement rate may be a rate of 50 to 100% daily replacement for a cell density of from 1x104 to 1x107 cells per ml of culture under physiologically acceptable conditions for the cells.