METHOD AND APPARATUS FOR ANALYZING AMINO ACID, PEPTIDE, PROTEIN, SACCHARIDE OR LIPID

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(11)

EP 1 477 800 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	published in accordance with Art. 158(3) EPC

(48)	Corrigendum issued on:
	23.02.2005 Bulletin 2005/08

(43)	Date of publication:
	17.11.2004 Bulletin 2004/47

(21)	Application number: 03703116.8

(22)	Date of filing: 31.01.2003

(51)	International Patent Classification (IPC)⁷: G01N 30/88, G01N 30/48, G01N 30/26, G01N 30/74, G01N 30/60, G01N 27/62

(86)	International application number:
	PCT/JP2003/000974

(87)	International publication number:
	WO 2003/065031 (07.08.2003 Gazette 2003/32)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT SE SI SK TR
	Designated Extension States:
	AL LT LV MK RO

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Priority:

31.01.2002 JP 2002024498

(71)	Applicant: GL Sciences Incorporated
	Tokyo 163-1130 (JP)

(72)	Inventors:
	KURODA, Ikuma, c/o GL Sciences Incorporated Iruma-shi, Saitama 358-0032 (JP) TAKAHASHI, Kouji, c/o GL Sciences Incorporated Iruma-shi, Saitama 358-0032 (JP) NAKAMURA, Hiroshi Chiba-shi, Chiba 262-0044 (JP)

(74)	Representative: Staeger - Sperling
	Müllerstrasse 3 80469 München 80469 München (DE)

(54)	METHOD AND APPARATUS FOR ANALYZING AMINO ACID, PEPTIDE, PROTEIN, SACCHARIDE OR LIPID

(57) The present invention aims to easily and simply perform selective detection/identification, extraction, separation, and purification of a phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein from a sample containing any one of them, comprising the steps of: retaining the phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein in a pretreatment column by passing the sample containing the phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein through a reversed phase HPLC column having a titanium dioxide column as the pretreatment column and retaining a non-phosphorylated amino acid, peptide, or protein in the reverse phase HPLC column; eluting the non-phosphorylated amino acid, peptide, or protein that is retained in the reversed phase HPLC column; eluting the phosphorylated amino acid, phosphorylated peptide, or phosphorylated protein retained in the pretreatment column with buffer; and detecting it.