| (19) |
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(11) |
EP 1 522 582 A8 |
| (12) |
CORRECTED EUROPEAN PATENT APPLICATION |
| (48) |
Corrigendum issued on: |
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08.06.2005 Bulletin 2005/23 |
| (88) |
Date of publication A3: |
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11.05.2005 Bulletin 2005/19 |
| (43) |
Date of publication: |
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13.04.2005 Bulletin 2005/15 |
| (22) |
Date of filing: 06.01.2000 |
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| (84) |
Designated Contracting States: |
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AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
| (30) |
Priority: |
07.01.1999 GB 9900298
|
| (62) |
Application number of the earlier application in accordance with Art. 76 EPC: |
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00900080.3 / 1141272 |
| (71) |
Applicant: MEDICAL RESEARCH COUNCIL |
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London W1N 4AL (GB) |
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| (72) |
Inventors: |
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- Griffiths, Andrew, MRC Lab. of Molecular Biology
Cambridge CB2 2QH (GB)
- Tawfik, Dan
Jerusalem 96264 (IL)
- Sepp, Armin, Gendaq Limited
London NW7 1AD (GB)
|
| (74) |
Representative: Crump, Julian Richard John et al |
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Mintz Levin Cohn Ferris Glovsky and Popeo Intellectual Property LLP The Rectory 9,
Ironmonger Lane
London EC2V 8EY
London EC2V 8EY (GB) |
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| |
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Remarks: |
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This application was filed on 08 - 11 - 2004 as a divisional application to the application
mentioned under INID code 62. |
|
| (54) |
Optical sorting method |
(57) A method for increasing the concentration of a nucleic acid molecule comprising the
steps of:
- (a) forming aqueous microcapsules from a water-in-oil emulsion, wherein a plurality
of microcapsules include a nucleic acid molecule, a bead capable of being linked to
the nucleic acid molecule and an aqueous solution comprising components necessary
to perform nucleic acid amplification;
- (b) amplifying the nucleic acid molecule in the microcapsules to form amplified product
copies of the nucleic acid molecule; and
- (c) capturing the amplified product copies to the bead in the microcapsules; thereby
increasing the concentration of the nucleic acid molecule.