(19)
(11) EP 1 530 633 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
18.08.2010 Bulletin 2010/33

(45) Mention of the grant of the patent:
23.06.2010 Bulletin 2010/25

(21) Application number: 03792391.9

(22) Date of filing: 20.08.2003
(51) International Patent Classification (IPC): 
C12N 15/10(2006.01)
(86) International application number:
PCT/EP2003/009223
(87) International publication number:
WO 2004/018673 (04.03.2004 Gazette 2004/10)

(54)

ISOLATION AND CLONING OF DNA FROM UNCULTIVATED ORGANISMS

ISOLIERUNG UND KLONIERUNG VON DNA AUS NICHT KULTIVIERTEN ORGANISMEN

ISOLEMENT ET CLONAGE DE L'ADN D'ORGANISMES NON CULTIVES


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

(30) Priority: 20.08.2002 EP 02018210

(43) Date of publication of application:
18.05.2005 Bulletin 2005/20

(73) Proprietor: B.R.A.I.N. Biotechnology Research And Information Network AG
64673 Zwingenberg (DE)

(72) Inventors:
  • QUAISER, Achim
    35000 Rennes (FR)
  • OCHSENREITER, Torsten
    Bern 3012 (CH)
  • TREUSCH, Alexander H.
    64409 Messel (DE)
  • KLETZIN, Arnulf
    64268 Darmstadt (DE)
  • SCHLEPER, Christa
    Klosterneuburg 3400 (AT)
  • LORENZ, Patrick
    64653 Lorsch (DE)
  • ECK, Jürgen
    Bensheim 64425 (DE)

(74) Representative: Vossius & Partner 
Siebertstrasse 4
81675 München
81675 München (DE)


(56) References cited: : 
WO-A-01/40497
WO-A-99/00168
WO-A-01/81357
US-A- 5 817 225
   
  • DATABASE WPI Section Ch, Week 198934 Derwent Publications Ltd., London, GB; Class B04, AN 1989-246867 XP002266614 & SU 1 439 124 A (ANTI-PLAGUE MIKROB), 23 November 1988 (1988-11-23)
  • MACNEIL I A ET AL: "EXPRESSION AND ISOLATION OF ANTIMICROBIAL SMALL MOLECULES FROM SOIL DNA LIBRARIES" JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, KOREAN SOCIETY FOR APPLIED MICROBIOLOGY, SEOUL, KO, vol. 3, no. 2, April 2001 (2001-04), pages 301-308, XP001050545 ISSN: 1017-7825 cited in the application
  • BÉJÀ O ET AL: "Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage." ENVIRONMENTAL MICROBIOLOGY. ENGLAND OCT 2000, vol. 2, no. 5, October 2000 (2000-10), pages 516-529, XP002277482 ISSN: 1462-2912 cited in the application
  • QUAISER ACHIM ET AL: "First insight into the genome of an uncultivated crenarchaeote from soil." ENVIRONMENTAL MICROBIOLOGY. ENGLAND OCT 2002, vol. 4, no. 10, October 2002 (2002-10), pages 603-611, XP002266613 ISSN: 1462-2912
  • ROOSE-AMSALEG C L ET AL: "Extraction and purification of microbial DNA from soil and sediment samples" APPLIED SOIL ECOLOGY, vol. 18, no. 1, September 2001 (2001-09), pages 47-60, XP002266612 ISSN: 0929-1393
  • LEBRETON M ET AL: "DEMONSTRATION OF EXTRACTION AND PCR AMPLIFICATION OF DNA FROM PHYTOPLANKTON OF LAKES WITH HIGH HUMIC ACID CONTENT" HYDROBIOLOGIA, JUNK, THE HAGUE, NL, vol. 438, 1 November 2000 (2000-11-01), pages 91-97, XP008026153 ISSN: 0018-8158
  • YOUNG C.C ET AL: "POLYVINYLPYRROLIDONE-AGAROSE GEL ELECTROPHORESIS PURIFICATION OF POLYMERASE CHAIN REACTION-AMPLIFIABLE DNA FROM SOILS" APPLIED & ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 6, 1 June 1993 (1993-06-01), pages 1972-1974, XP000983454 AMERICAN SOCIETY FOR MICROBIOLOGY, US
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).