(19)

(11)

EP 1 543 128 A2

(12)

(88) Date of publication A3: 21.04.2005 (43) Date of publication: 22.06.2005 Bulletin 2005/25 (21) Application number: 03765705.3 (22) Date of filing: 18.07.2003

(51) International Patent Classification (IPC)7: C12N 15/74 (86) International application number: PCT/US2003/022437 (87) International publication number: WO 2004/009768 (29.01.2004 Gazette 2004/05)

(84) Designated Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR Designated Extension States: AL LT LV MK (30) Priority: 18.07.2002 US 396335 P 26.07.2002 US 398617 P 19.11.2002 US 427231 P 24.03.2003 US 456496 P 03.06.2003 US 474940 P (71) Applicant: INVITROGEN CORPORATION Carlsbad, CA 92008 (US)	(72) Inventors: BENNETT, Robert, P. Encinitas, CA 92024 (US) WELCH, Peter, J. San Diego, CA 92116 (US) HARWOOD, Steven Oceanside, CA 92057 (US) MADDEN, Knut Carlsbad, CA 92008 (US) FRIMPONG, Kenneth San Diego, CA 92122 (US) FRANKE, Kenneth, E. Gaithersburg, MD 20878 (US) (74) Representative: Adams, Harvey Vaughan John et al Mathys & Squire,120 Holborn London EC1N 2SQ London EC1N 2SQ (GB)