(19)
(11) EP 1 572 984 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
01.06.2016 Bulletin 2016/22

(45) Mention of the grant of the patent:
02.03.2016 Bulletin 2016/09

(21) Application number: 03813286.6

(22) Date of filing: 07.12.2003
(51) International Patent Classification (IPC): 
C12N 5/00(2006.01)
(86) International application number:
PCT/IL2003/001030
(87) International publication number:
WO 2004/055155 (01.07.2004 Gazette 2004/27)

(54)

FEEDER-FREE, XENO-FREE CULTURE SYSTEM FOR HUMAN EMBRYONIC STEM CELLS

FEEDERZELLFREI, XENOFREI KULTURSYSTEM FÜR MENSCHLICHER EMBRYONALER STAMMZELLEN

SYSTÈME DE CULTURE POUR CELLULES SOUCHES EMBRYONNAIRES HUMAINES SANS CELLULES NOURRICIERES NI COMPOSANTS XÉNOGÈNES

(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

(30) Priority: 16.12.2002 US 433619 P

(43) Date of publication of application:
14.09.2005 Bulletin 2005/37

(60) Divisional application:
11190776.2 / 2457999

(73) Proprietor: Technion Research & Development Foundation Ltd.
32000 Haifa (IL)

(72) Inventors:
  • AMIT, Michal
    20 142 Misgav (IL)
  • ITSKOVITZ-ELDOR, Joseph
    34 98701 Haifa (IL)

(74) Representative: Dennemeyer & Associates S.A. 
Poccistrasse 11
80336 München
80336 München (DE)


(56) References cited: : 
WO-A-01/51616
 WO-A-03/029443
   
  • XU C ET AL: "Feeder-free growth of undifferentiated human embryonic stem cells." NATURE BIOTECHNOLOGY OCT 2001, vol. 19, no. 10, October 2001 (2001-10), pages 971-974, XP002282070 ISSN: 1087-0156
  • GOLDSBOROUGH ET AL: 'Serum-free culture of murine embryonic stem cells Focus' vol. 20, no. 1, 1998, pages 9 - 12
  • AMIT ET AL: 'Feeder layer- and serum-free culture of human embryonic stem cells' BIOL. OF REPROD. vol. 70, 2004, pages 837 - 845, XP002978624
  • AMIT ET AL: 'Human feeder layers for human embryonic stem cells' BIOL. OF REPROD. vol. 68, 2003, pages 2150 - 2156, XP002978486
  • PEI ET AL: 'Serum-free culture of rhesus monkey embryonic stem cells' ARCH. ANDROL. vol. 49, 2003, pages 331 - 342, XP002978622
  • MURDOCH ET AL: 'Human embryonic-derived hematopoietic repopulating cells require distinct factors to sustain in vivo repopulating function' EXP. HEMATOL. vol. 30, 2002, pages 598 - 605, XP002978487
  • AMIT ET AL: 'Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture' DEV. BIOL. vol. 227, 2000, pages 271 - 278, XP001145851
  • ROBERTSON D: "NIH sacrifices commercial rights in WiCell deal.", NATURE BIOTECHNOLOGY, vol. 19, no. 11, November 2001 (2001-11), page 1001, ISSN: 1087-0156
  • ZHANG X ET AL: "Feeder layer- and serum-free culture of rhesus monkey embryonic stem cells", REPRODUCTIVE BIOMEDICINE ONLINE, vol. 13, no. 3, 1 January 2006 (2006-01-01), pages 412-420, XP027052307, ISSN: 1472-6483 [retrieved on 2006-01-01]
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).