(19)
(11) EP 1 657 309 A8

(12) CORRECTED EUROPEAN PATENT APPLICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 A1)

(48) Corrigendum issued on:
02.06.2010 Bulletin 2010/22

(43) Date of publication:
17.05.2006 Bulletin 2006/20

(21) Application number: 06002556.6

(22) Date of filing: 04.05.1999
(51) International Patent Classification (IPC): 
C12N 15/86(2006.01)
C12N 5/10(2006.01)
 C12M 3/00(2006.01)
(84) Designated Contracting States:
AT BE CH DE DK ES FI FR GB GR IE IT LI LU NL PT SE
Designated Extension States:
LT LV RO

(30) Priority: 04.05.1998 US 73076

(62) Application number of the earlier application in accordance with Art. 76 EPC:
99921681.5 / 1078095

(71) Applicant: CANJI, Inc.
San Diego, CA 92121 (US)

(72) Inventors:
  • Giroux, Daniel D.
    Cardiff-by-the-sea, CA 92007 (US)
  • Goudreau, Ann M.
    San Diego, CA 92131 (US)
  • Ramachandra, Muralidhara
    Koramangala Bangalore - 560034 (IN)
  • Shabram, Paul W.
    Olivenhain, CA 92024 (US)

(74) Representative: UEXKÜLL & STOLBERG 
Patentanwälte Beselerstraße 4
22607 Hamburg
22607 Hamburg (DE)

 
Remarks:
This application was filed on 08.02.2006 as a divisional application to the application mentioned under INID code 62.
 


(54) Viral production process


(57) The present invention is directed to a method of producing recombinant viral vectors at high titers incorporating a variety of important advancements over the art. The method of the present invention incorporates multiple features which provide enhanced production of viruses, particularly those viruses encoding exogenous transgenes. The specifically illustrated method describes a method for the high titer serum-free media production of recombinant replication defective adenoviruses containing an exogenous transgene. The invention provides methods of preparing microcarriers, methods, for seeding bioreactors at high cell density, increasing the infectivity of the producer cells to the virus, methods to increase product yield through synchronization of the cell cycle of the producer cells, and methods to minimize the deleterious effects of exogenous transgenes. The invention further provides producer cells prepared by the process of the invention. The invention further provides viruses produced by the process.