PYRROLOQUINOLINE QUINONE (PQQ)-DEPENDENT GLUCOSE DEHYDROGENASE MODIFICATION HAVING EXCELLENT SUBSTRATE SPECIFICITY

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EP 1 666 586 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	published in accordance with Art. 158(3) EPC

(15)	Correction information:
	Corrected version no 1 (W1 A1)

(48)	Corrigendum issued on:
	04.10.2006 Bulletin 2006/40

(43)	Date of publication:
	07.06.2006 Bulletin 2006/23

(21)	Application number: 04772464.6

(22)	Date of filing: 31.08.2004

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International Patent Classification (IPC):

C12N 9/04^(2006.01)
C12N 5/10^(2006.01)
C12N 1/19^(2006.01)
C12Q 1/32^(2006.01)
G01N 33/66^(2006.01)

C12N 15/53^(2006.01)
C12N 1/15^(2006.01)
C12N 1/21^(2006.01)
C12M 1/40^(2006.01)

(86)	International application number:
	PCT/JP2004/012508

(87)	International publication number:
	WO 2005/026340 (24.03.2005 Gazette 2005/12)

(84)	Designated Contracting States:
	DE FR GB IT

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Priority:

08.09.2003 JP 2003315797
08.09.2003 JP 2003315799
04.03.2004 JP 2004060283
04.03.2004 JP 2004060282
21.05.2004 JP 2004151905

(71)	Applicant: Toyo Boseki Kabushiki Kaisha
	Osaka-shi, Osaka 530-8230 (JP)

(72)	Inventors:
	Takeshima, Seiji, c/o Toyo Boseki Kabushiki Kaisha Tsuruga-shi, Fukui 914-0047 (JP) Matsumura, Tadanobu, c/o Toyo Boseki K. K. Tsuruga-shi, Fukui 914-0047 (JP) Kishimoto, Takahide, c/o Toyo Boseki K. K. Tsuruga-shi, Fukui 914-0047 (JP) Oka, Masanori, c/o Toyo Boseki Kabushiki Kaisha Tsuruga-shi, Fukui 914-0047 (JP) Hirayama, Noriaki Zama-shi, Kanagawa 228-0023 (JP)

(74)	Representative: Vossius & Partner
	Siebertstrasse 4 81675 München 81675 München (DE)

(54)	PYRROLOQUINOLINE QUINONE (PQQ)-DEPENDENT GLUCOSE DEHYDROGENASE MODIFICATION HAVING EXCELLENT SUBSTRATE SPECIFICITY

(57) PQQGDH having an improved substrate specificity or having an improved specific activity in an assay system using ferricyanide ion as a mediator is provided. Modified PQQGDH having the enhanced substrate specificity by introducing an amino acid mutation in a particular region of PQQGDH, and a method of enhancing the specific activity compared with a wild type in the assay system using the ferricyanide ion as the mediator by deleting, substituting, or adding one or more amino acids in an amino acid sequence of the wild type pyrroloquinoline quinone dependent glucose dehydrogenase.