(19)
(11) EP 1 668 136 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
21.03.2012 Bulletin 2012/12

(45) Mention of the grant of the patent:
19.10.2011 Bulletin 2011/42

(21) Application number: 04770715.3

(22) Date of filing: 27.08.2004
(51) International Patent Classification (IPC): 
C12N 15/82(2006.01)
C12N 9/64(2006.01)
 C12N 15/62(2006.01)
(86) International application number:
PCT/IS2004/000011
(87) International publication number:
WO 2005/021764 (10.03.2005 Gazette 2005/10)

(54)

A PROCESS FOR PROTEOLYTIC CLEAVAGE AND PURIFICATION OF RECOMBINANT PROTEINS PRODUCED IN PLANTS

VERFAHREN ZUR PROTEOLYTISCHEN SPALTUNG UND REINIGUNG VON IN PFLANZEN PRODUZIERTEN REKOMBINANTEN PROTEINEN

PROCESSUS DE CLIVAGE PROTEOLYTIQUE ET PURIFICATION DE PROTEINES DE RECOMBINAISON

(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR
Designated Extension States:
AL HR LT LV MK

(30) Priority: 27.08.2003 US 497935 P
27.08.2003 IS 692903

(43) Date of publication of application:
14.06.2006 Bulletin 2006/24

(73) Proprietor: ORF Líftaekni hf.
203 Kopavogur (IS)

(72) Inventors:
  • MANTYLA, Einar
    IS-104 Reykjavik (IS)
  • ORVAR, Bjorn, Larus
    IS-200 Kopavogur (IS)

(74) Representative: Arnason Faktor 
Intellectual Property Consulting Gudridarstig 2-4
113 Reykjavik
113 Reykjavik (IS)


(56) References cited: : 
WO-A-00/77174
US-A- 6 048 715
 WO-A-02/05922
US-A1- 2002 164 718
   
  • BORASTON ALISDAIR B ET AL: "A family 2a carbohydrate-binding module suitable as an affinity tag for proteins produced in Pichia pastoris" PROTEIN EXPRESSION AND PURIFICATION, vol. 21, no. 3, April 2001 (2001-04), pages 417-423, XP002321711 ISSN: 1046-5928
  • BORASTON ALISDAIR B ET AL: "Binding specificity and thermodynamics of a family 9 carbohydrate-binding module from Thermotoga maritima xylanase 10A" BIOCHEMISTRY, vol. 40, no. 21, 29 May 2001 (2001-05-29), pages 6240-6247, XP002321709 ISSN: 0006-2960 cited in the application
  • REEVES R A ET AL: "Sequencing and expression of additional xylanase genes from the hyperthermophile Thermotoga maritima FjSS3B.1." APPLIED AND ENVIRONMENTAL MICROBIOLOGY. APR 2000, vol. 66, no. 4, April 2000 (2000-04), pages 1532-1537, XP002321710 ISSN: 0099-2240
  • CHHABRA S R ET AL: "Biochemical characterization of Thermotoga maritima endoglucanase Cel74 with and without a carbohydrate binding module (CBM)" FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 531, no. 2, 6 November 2002 (2002-11-06), pages 375-380, XP004391664 ISSN: 0014-5793
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).