EXPRESSION OF GRANULAR STARCH HYDROLYZING ENZYMES IN TRICHODERMA AND PROCESS FOR PRODUCING GLUCOSE FROM GRANULAR STARCH SUBSTRATES

(19)

(11)

EP 1 685 244 B8

(12)	CORRECTED EUROPEAN PATENT SPECIFICATION
	Note: Bibliography reflects the latest situation

(15)	Correction information:
	Corrected version no 1 (W1 B1)

(48)	Corrigendum issued on:
	25.04.2012 Bulletin 2012/17

(45)	Mention of the grant of the patent:
	21.03.2012 Bulletin 2012/12

(21)	Application number: 04811428.4

(22)	Date of filing: 18.11.2004

(51)

International Patent Classification (IPC):

C12N 9/34^(2006.01)
C12P 19/00^(2006.01)
C08B 30/00^(2006.01)

C12N 9/62^(2006.01)
C12P 19/14^(2006.01)

(86)	International application number:
	PCT/US2004/038713

(87)	International publication number:
	WO 2005/052148 (09.06.2005 Gazette 2005/23)

(54)

EXPRESSION OF GRANULAR STARCH HYDROLYZING ENZYMES IN TRICHODERMA AND PROCESS FOR PRODUCING GLUCOSE FROM GRANULAR STARCH SUBSTRATES

EXPRESSION GRANULÄRE STÄRKE HYDROLYSIERENDER ENZYME IN TRICHODERMA UND VERFAHREN ZUR HERSTELLUNG VON GLUCOSE AUS GRANULÄREN STÄRKESUBSTRATEN

EXPRESSION D'ENZYMES D'HYDROLYSE D'AMIDON GRANULAIRE DE TRICHODERMA ET PROCEDE DE PRODUCTION DE GLUCOSE A PARTIR DE SUBSTRATS D'AMIDON GRANULAIRE

(84)	Designated Contracting States:
	AT BE CH CY CZ DE DK ES FI FR GB HU IE IS IT LI LU MC NL RO SE SI SK TR

(30)

Priority:

21.11.2003 US 524279 P
22.12.2003 US 531953 P
28.04.2004 US 566358 P

(43)	Date of publication of application:
	02.08.2006 Bulletin 2006/31

(60)	Divisional application:
	06009350.7 / 1698692

(73)	Proprietor: Danisco US Inc.
	Palo Alto, CA 94304 (US)

(72)

Inventors:

BALDWIN, Toby, L. c/o Genencor International, Inc.
Palo Alto, CA 94304-1013 (US)
BOWER, Benjamin S., c/o Genencor Intern., Inc.
Palo Alto, CA 94304-1013 (US)
CHOTANI, Gopal, K c/o Genencor International, Inc.
Palo Alto, CA 94304-1013 (US)
DUNN-COLEMAN, Nigel c/o Genencor Intern., Inc.
Palo Alto, CA 94304-1013 (US)
LANTERO, Jr., Oreste c/o Genencor Intern., Inc.
Palo Alto, CA 94304-1013 (US)
LANTZ, Suzanne, E c/o Genencor International, Inc.
Palo Alto, CA 94304-1013 (US)
PEPSIN, Michael, J. c/o Genencor Intern., Inc.
Palo Alto, CA 94304-1013 (US)
SHETTY, Jayarama, K. c/o Genencor Intern., Inc.
Palo Alto, CA 94304-1013 (US)
STROHM, Bruce, A. c/o Genencor International, Inc.
Palo Alto, CA 94304-1013 (US)

(74)	Representative: Kiddle, Simon John et al
	Mewburn Ellis LLP 33 Gutter Lane London EC2V 8AS London EC2V 8AS (GB)

(56)

References cited: :

EP-A- 0 215 594
WO-A-99/60136

EP-A- 0 625 577
WO-A-03/068976

KARAKATSANIS A ET AL: "HYDROLYSIS OF VARIOUS STARCHES BY THE SYNERGISTIC ACTION OF ALPHA- AMYLASE AND GLUCOAMYLASE IN AQUEOUS TWO PHASE IMPELLER AGITATED SYSTEMS" STARKE - STARCH, WILEY-VCH VERLAG, WEINHEIM, DE, vol. 49, no. 5, 1 May 1997 (1997-05-01), pages 194-199, XP000692723 ISSN: 0038-9056
FUJII M ET AL: "SYNERGISTIC ACTION OF ALPHA AMYLASE AND GLUCOAMYLASE ON HYDROLYSIS OF STARCH" BIOTECHNOLOGY AND BIOENGINEERING, vol. 27, no. Y, 1985, pages 260-265, XP008083287 ISSN: 0006-3592
LIAKOPOULOU-KYRIAKIDES MARIA ET AL: "Synergistic hydrolysis of crude corn starch by alpha-amylases and glucoamylases of various origins" CEREAL CHEMISTRY, vol. 78, no. 5, September 2001 (2001-09), pages 603-607, XP008083292 ISSN: 0009-0352
ARASARATNAM VASANTHI ET AL: "Synergistic action of alpha-amylase and glucoamylase on raw corn" STARCH, vol. 45, no. 6, 1993, pages 231-233, XP002449550 ISSN: 0038-9056
FAGERSTROM RICHARD: "Purification and specificity of recombinant Hormoconis resinae glucoamylase P and endogenous glucoamylase from Trichoderma reesei" ENZYME AND MICROBIAL TECHNOLOGY, vol. 16, no. 1, 1994, pages 36-42, XP002325130 ISSN: 0141-0229
WIEBE MARILYN G ET AL: "Growth-rate-independent production of recombinant glucoamylase by Fusarium venenatum JeRS 325" BIOTECHNOLOGY AND BIOENGINEERING, vol. 68, no. 3, 5 May 2000 (2000-05-05), pages 245-251, XP002325131 ISSN: 0006-3592
WITHERS JULIE M ET AL: "Optimization and stability of glucoamylase production by recombinant strains of Aspergillus niger in chemostat culture" BIOTECHNOLOGY AND BIOENGINEERING, vol. 59, no. 4, 20 August 1998 (1998-08-20), pages 407-418, XP002325132 ISSN: 0006-3592
DATABASE UniProt [Online] 1 November 1996 (1996-11-01), "Glucoamylase (EC 3.2.1.3)." XP002325133 retrieved from EBI accession no. UNIPROT:Q12623 Database accession no. Q12623

Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).