(19)

(11)

EP 1 706 483 A2

(12)

(88) Date of publication A3: 09.09.2005 (43) Date of publication: 04.10.2006 Bulletin 2006/40 (21) Application number: 04813310.2 (22) Date of filing: 03.12.2004

(51) International Patent Classification (IPC):  C12N 5/00(2006.01) (86) International application number: PCT/US2004/040977 (87) International publication number: WO 2005/056765 (23.06.2005 Gazette 2005/25)

(84) Designated Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR (30) Priority: 08.12.2003 US 527920 P (71) Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE Cambridge, MA 02138 (US)	(72) Inventors: GREEN, Howard Boston, MA 02115 (US) IUCHI, Shiro Boston, MA 02115 (US) EASLEY, Karen Boston, MA 02115 (US) (74) Representative: Jump, Timothy John Simon et al Venner Shipley LLP 20 Little Britain London EC1A 7DH London EC1A 7DH (GB)