(19)
(11) EP 1 767 624 A8

(12) CORRECTED EUROPEAN PATENT APPLICATION
published in accordance with Art. 158(3) EPC

(15) Correction information:
Corrected version no 1 (W1 A1)

(48) Corrigendum issued on:
18.07.2007 Bulletin 2007/29

(43) Date of publication:
28.03.2007 Bulletin 2007/13

(21) Application number: 05745968.7

(22) Date of filing: 26.05.2005
(51) International Patent Classification (IPC): 
C12N 15/09(2006.01)
C12N 1/19(2006.01)
C12N 5/10(2006.01)
C12P 13/02(2006.01)
 C12N 1/15(2006.01)
C12N 1/21(2006.01)
C12N 9/88(2006.01)
(86) International application number:
PCT/JP2005/010107
(87) International publication number:
WO 2005/116206 (08.12.2005 Gazette 2005/49)
(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR

(30) Priority: 26.05.2004 JP 2004156593
18.08.2004 JP 2004237888

(71) Applicant: MITSUBISHI RAYON CO., LTD.
Tokyo 108-8506 (JP)

(72) Inventors:
  • WATANABE, Fumiaki, Mitsubishi Rayon Co., Ltd.
    Yokohama-shi, Kanagawa 2300053 (JP)
  • UJIHARA, Dai
    Ashigarakami-gun, Kanagawa 258-0021 (JP)
  • SAKAI, Miki, Mitsubishi Rayon Co., Ltd.
    Minato-ku, Tokyo 1088506 (JP)
  • YU, Fujio, Mitsubishi Rayon Co., Ltd.
    Yokohama-shi, Kanagawa 2300053 (JP)
  • NAKAMURA, Tetsuji, Mitsubishi Rayon Co., Ltd.
    Yokohama-shi, Kanagawa 2300053 (JP)

(74) Representative: HOFFMANN EITLE 
Patent- und Rechtsanwälte Arabellastrasse 4
81925 München
81925 München (DE)


(56) References cited: : 
   
       


    (54) IMPROVED NITRILE HYDRATASE


    (57) The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.