(19)
(11) EP 1 776 467 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
28.10.2015 Bulletin 2015/44

(45) Mention of the grant of the patent:
23.09.2015 Bulletin 2015/39

(21) Application number: 05767491.3

(22) Date of filing: 29.07.2005
(51) International Patent Classification (IPC): 
C12Q 1/48(2006.01)
C12Q 1/68(2006.01)
C12Q 1/66(2006.01)
(86) International application number:
PCT/GB2005/002998
(87) International publication number:
WO 2006/010948 (02.02.2006 Gazette 2006/05)

(54)

METHOD FOR DETERMINING THE AMOUNT OF TEMPLATE NUCLEIC ACID PRESENT IN A SAMPLE

VERFAHREN ZUR BESTIMMUNG DER MENGE AN IN EINER PROBE ENTHALTENER MATRIZENNUKLEINSÄURE

PROCEDE PERMETTANT DE DETERMINER LA QUANTITE D'ACIDE NUCLEIQUE MATRICE PRESENT DANS UN ECHANTILLON


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
Designated Extension States:
AL BA HR MK YU

(30) Priority: 29.07.2004 GB 0416944

(43) Date of publication of application:
25.04.2007 Bulletin 2007/17

(73) Proprietor: Lumora Limited
Ely CB7 4EA (GB)

(72) Inventors:
  • TISI, Laurence Carlo
    Ely CB6 1DP (GB)
  • MURRAY, James, Augustus, Henry
    Penarth CF64 3BD Wales (GB)
  • GANDELMAN, Olga
    Cambridge CB4 2HE (GB)
  • ARMSTRONG, Victoria Louise Church
    Santa Clarita CA 91390 (US)

(74) Representative: Hallybone, Huw George et al
Carpmaels & Ransford LLP One Southampton Row
London WC1B 5HA
London WC1B 5HA (GB)


(56) References cited: : 
WO-A-98/55653
US-A1- 2004 175 709
WO-A-02/090586
   
  • ARAKAWA H ET AL: "Detection of cariogenic bacteria genes by a combination of allele-specific polymerase chain reactions and a novel bioluminescent pyrophosphate assay" ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, NEW YORK, NY, US, vol. 333, no. 2, 15 October 2004 (2004-10-15), pages 296-302, XP004573018 ISSN: 0003-2697
  • ARAKAWA H ET AL: "A new assay for determining pyrophosphate using pyruvate phosphate dikinase and its application to DNA analysis" LUMINESCENCE (CHICHESTER), vol. 19, no. 3, May 2004 (2004-05), page 132, XP009058022 & 13TH INTERNATIONAL SYMPOSIUM ON BIOLUMINESCENCE AND CHEMILUMINESCENCE; YOKOHAMA, JAPAN; AUGUST 02-06, 2004 ISSN: 1522-7235
  • MAEDA MASAKO: "[Determination of biological substances using bioluminescent reaction based on luciferin-luciferase]" RINSHO BYORI. THE JAPANESE JOURNAL OF CLINICAL PATHOLOGY. JUL 2004, vol. 52, no. 7, July 2004 (2004-07), pages 595-603, XP009058028 ISSN: 0047-1860
  • IMAMURA O ET AL: "Simple and rapid bioluminescent detection of two verotoxin genes using allele-specific PCR of E. coli O157: H7." LUMINESCENCE (CHICHESTER), vol. 18, no. 2, 21 January 2003 (2003-01-21), pages 107-112, XP009058023 ISSN: 1522-7235
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).