17,20(Z)-DEHYDRO VITAMIN D ANALOGS AND THEIR USES

Description

BACKGROUND OF THE INVENTION

[0001] This invention relates to vitamin D compounds, and more particularly to 17,20(Z)-dehydro vitamin D analogs and their pharmaceutical uses, and especially 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-norvitamin D₃, its biological activities, and its pharmaceutical uses.

[0002] The natural hormone, 1α,25-dihydroxyvitamin D₃ and its analog in the ergosterol series, i.e. 1α,25-dihydroxyvitamin D₂ are known to be highly potent regulators of calcium homeostasis in animals and humans, and their activity in cellular differentiation has also been established, Ostrem et al., Proc. Natl. Acad. Sci. USA, 84, 2610 (1987). Many structural analogs of these metabolites have been prepared and tested, including 1α-hydroxyvitamin D₃, 1α-hydroxyvitamin D₂, various side chain homologated vitamins and fluorinated analogs. Some of these compounds exhibit an interesting separation of activities in cell differentiation and calcium regulation. This difference in activity may be useful in the treatment of a variety of diseases such as renal osteodystrophy, vitamin D-resistant rickets, osteoporosis, psoriasis, and certain malignancies.

[0003] Another class of vitamin D analogs, i.e. the so called 19-nor-vitamin D compounds, is characterized by the replacement of the A-ring exocyclic methylene group (carbon 19), typical of the vitamin D system, by two hydrogen atoms. Biological testing of such 19-nor-analogs (e.g., 1α,25-dihydroxy-19-nor-vitamin D₃) revealed a selective activity profile with high potency in inducing cellular differentiation, and very low calcium mobilizing activity. Thus, these compounds are potentially useful as therapeutic agents for the treatment of malignancies, or the treatment of various skin disorders. Two different methods of synthesis of such 19-nor-vitamin D analogs have been described (Perlman et al., Tetrahedron Lett. 31, 1823 (1990); Perlman et al., Tetrahedron Lett. 32, 7663 (1991), and DeLuca et al., U.S. Pat. No. 5,086,191).

[0004] In U.S. Pat. No. 4,666,634, 2β-hydroxy and alkoxy (e.g., ED-71) analogs of 1α,25-dihydroxyvitamin D₃ have been described and examined by Chugai group as potential drugs for osteoporosis and as antitumor agents. See also Okano et al., Biochem. Biophys. Res. Commun. 163, 1444 (1989). Other 2-substituted (with hydroxyalkyl, e.g., ED-120, and fluoroalkyl groups) A-ring analogs of 1α,25-dihydroxyvitamin D₃ have also been prepared and tested (Miyamoto et al., Chem. Pharm. Bull. 41, 1111 (1993); Nishii et al., Osteoporosis Int. Suppl. 1, 190 (1993); Posner et al., J. Org. Chem. 59, 7855 (1994), and J. Org. Chem. 60, 4617 (1995)).

[0005] 2-substituted analogs of 1α,25-dihydroxy-19-nor-vitamin D₃ have also been synthesized, i.e. compounds substituted at 2-position with hydroxy or alkoxy groups (DeLuca et al., U.S. Pat. No. 5,536,713), with 2-alkyl groups (DeLuca et al U.S. Patent No. 5,945,410), and with 2-alkylidene groups (DeLuca et al U.S. Patent No. 5,843,928), which exhibit interesting and selective activity profiles. All these studies indicate that binding sites in vitamin D receptors can accommodate different substituents at C-2 in the synthesized vitamin D analogs.

[0006] In a continuing effort to explore the 19-nor class of pharmacologically important vitamin D compounds, analogs which are characterized by the presence of a methylene substituent at carbon 2 (C-2), a hydroxyl group at carbon 1 (C-1), and a shortened side chain attached to carbon 20 (C-20) have also been synthesized and tested. 1α-hydroxy-2-methylene-19-nor-pregnacalciferol is described in U.S. Patent 6,566,352 while 1α-hydroxy-2-methylene-19-nor-homopregnacalciferol is described in U.S. Patent 6,579,861 and 1α-hydroxy-2-methylene-19-nor-bishomopregnacalciferol is described in U.S. Patent 6,627,622. All three of these compounds have relatively high binding activity to vitamin D receptors and relatively high cell differentiation activity, but little if any calcemic activity as compared to 1α,25-dihydroxyvitamin D₃. Their biological activities make these compounds excellent candidates for a variety of pharmaceutical uses, as set forth in the '352, '861 and '622 patents.

[0007] Further vitamin D analogs are disclosed in WO94/01398, WO98/24762 and WO99/18070.

SUMMARY OF THE INVENTION

[0008] The present invention is directed toward 17,20(Z)-dehydro vitamin D analogs, and their pharmaceutical uses, and more specifically toward 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-norvitamin D₃, their biological activity, and various pharmaceutical uses for these compounds.

[0009] Structurally these 17,20(Z)-dehydro-vitamin D analogs are characterized by the general formula I shown below:

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group -(CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R
a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and -CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where-R⁵ represents-hydrogen, hydroxy, protected hydroxy, or C_1-5 alkyl.

[0010] The preferred analog is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-norvitamin D₃ which has the following formula Ia:

[0011] The above compounds of formula I, especially formula Ia, exhibit a desired, and highly advantageous, pattern of biological activity. These compounds are characterized by relatively high binding to vitamin D receptors, as well as relatively high intestinal calcium transport activity, as compared to that of 1α,25-dihydroxyvitamin D₃, and they also exhibit relatively high activity in their ability to mobilize calcium from bone, as compared to 1α,25-dihydroxyvitamin D₃. Hence, these compounds can be characterized as having relatively high calcemic activity. Their preferential activity on intestinal calcium transport and calcium mobilizing activity allows the in vivo administration of these compounds for the treatment and prophylaxis of metabolic bone diseases. Because of their preferential calcemic activity on gut calcium transport and on bone, these compounds would be preferred therapeutic agents for the treatment and prophylaxis of diseases such as osteoporosis, especially low bone turnover osteoporosis, steroid induced osteoporosis, senile osteoporosis or postmenopausal osteoporosis, as well as osteomalacia and renal osteodystrophy. These analogs having relatively high calcemic activity while being very active on cell differentiation are also expected to be useful as a therapy to treat hypoparathyroidism since they are effective to raise blood calcium levels.

[0012] The compounds I, and particularly Ia, of the invention have also been discovered to be especially suited for treatment and prophylaxis of human disorders which are characterized by an imbalance in the immune system, e.g. in autoimmune diseases, including multiple sclerosis, lupus, diabetes mellitus, host versus graft rejection, and rejection of organ transplants; and additionally for the treatment of inflammatory diseases, such as rheumatoid arthritis, asthma, and inflammatory bowel diseases such as celiac disease, ulcerative colitis and Crohn's disease. Acne, alopecia and hypertension are other conditions which may be treated with the compounds of the invention.

[0013] The above compounds I, and particularly Ia, are also characterized by relatively high cell differentiation activity. Thus, these compounds also provide therapeutic agents for the treatment of psoriasis, or as an anti-cancer agent, especially against leukemia, colon cancer, breast cancer, skin cancer and prostate cancer. In addition, due to their relatively high cell differentiation activity, these compounds provide therapeutic agents for the treatment of various skin conditions including wrinkles, lack of adequate dermal hydration, i.e. dry skin, lack of adequate skin firmness, i.e. slack skin, and insufficient sebum secretion. Use of these compounds thus not only results in moisturizing of skin but also improves the barrier function of skin.

[0014] The compounds of the invention of formula I, and particularly formula Ia, are also useful in preventing or treating obesity, inhibiting adipocyte differentiations, inhibiting SCD-1 gene transcription, and/or reducing body fat in animal subjects.
Therefore, in some embodiments, a method of preventing or treating obesity, inhibiting adipocyte differentiations, inhibiting SCD-1 gene transcription, and/or reducing body fat in an animal subject includes administering to the animal subject, an effective amount of one or more of the compounds or a pharmaceutical composition that includes one or more of the compounds of formula I, and in particular the compound of formula Ia.
Administration of one or more of the compounds or the pharmaceutical compositions to the subject inhibits adipocyte differentiation, inhibits gene transcription, and/or reduces body fat in the animal subject.

[0015] One or more of the compounds may be present in a composition to treat or prevent the above-noted diseases and disorders in an amount from about 0.01 µg/gm to about 1000 µg/gm of the composition, preferably from about 0.1 µg/gm to about 500µg/gm of the composition, and may be administered topically, transdermally, orally, rectally, nasally, sublingually, or parenterally in dosages of from about 0.01 µg/day to about 1 000µg/day, preferably from about 0.1 µg/day to about 500 µg/day.

BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figures 1-5 illustrate various biological activities of 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-norvitamin D₃, hereinafter referred to as "VIT-III," as compared to the native hormone 1α,25-dihydroxyvitamin D₃, hereinafter "1,25(OH)₂D₃."

Figure 1 is a graph illustrating the relative activity of VIT-III and 1,25(OH)₂D₃ to compete for binding with [³H]-1,25-(OH)₂-D₃ to the full-length recombinant rat vitamin D receptor;

Figure 2 is a graph illustrating the percent HL-60 cell differentiation as a function of the concentration of VIT-III and 1,25(OH)₂D₃;

Figure 3 is a graph illustrating the in vitro transcription activity of 1,25(OH)₂D₃ as compared to VIT-III;

Figure 4 is a bar graph illustrating the bone calcium mobilization activity of 1,25(OH)₂D₃ as compared to VIT-III; and

Figure 5 is a bar graph illustrating the intestinal calcium transport activity of 1,25 (OH)₂D₃ as compared to VIT-III.

DETAILED DESCRIRTION OF THE INVENTION

[0017] The preparation of 17,20(Z)-dehydro vitamin D analogs having the structure I can be accomplished by a common general method, i.e. the condensation of a bicyclic Windaus-Grundmann type ketone II with the allylic phosphine oxide III to the corresponding 17,20(Z)-dehydro vitamin D analog IV followed by deprotection at C-1 and C-3 to provide I:

In the structures III and IV, groups Y₁ and Y₂ are hydroxy-protecting groups, preferably t-butyldimethylsilyl, it being also understood that any functionalities that might be sensitive, or that interfere with the condensation reaction, be suitably protected as is well-known in the art. The process shown above represents an application of the convergent synthesis concept, which has been applied effectively for the preparation of vitamin D compounds [e.g. Lythgoe et al., J. Chem. Soc. Perkin Trans. I, 590 (1978); Lythgoe, Chem. Soc. Rev. 9, 449 (1983); Toh et al., J. Org. Chem. 48, 1414 (1983); Baggiolini et al., J. Org. Chem. 51, 3098 (1986); Sardina et al,. J. Org. Chem. 51, 1264 (1986); J. Org. Chem. 51, 1269 (1986); DeLuca et al., U.S. Pat. No. 5,086,191; DeLuca et al., U.S. Pat. No. 5,536,713].

[0018] The hydrindanones of the general structure II are not known. They can be prepared by the method shown in the Schemes herein (see the preparation of compound VIT-III).

[0019] For the preparation of the required phosphine oxides of general structure III, a synthetic route has been developed starting from a methyl quinicate derivative which is easily obtained from commercial (IR,3R,4S,5R)-(-)-quinic acid as described by Perlman et al., Tetrahedron Lett. 32, 7663 (1991) and DeLuca et al., U.S. Pat. No. 5,086,191.

[0020] The overall process of the synthesis of compounds I and Ia is illustrated and described more completely in U.S. Patent No. 5,843,928 entitled "2-Alkylidene-19-Nor-Vitamin D Compounds" the specification of which is specifically incorporated herein by reference.

[0021] Particularly preferred 17,20(Z)-dehydro vitamin D analogs are those encompassed by general formula I wherein carbon-2 on the A-ring is substituted with an alkylidene group or an alkyl group, or are hydrolyzable slow release compounds (whether substituted at carbon-2 or not substituted at carbon-2).

2-Alkylidene Compounds

[0022] Structurally these 2-alkylidene analogs are characterized by the general formula V shown below:

where Y₁, Y₂, R₁₁, R₁₂ and Z are as previously defined herein, and R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl and fluoroalkyl, or, when taken together represent the group -(CH₂)_x- where x is an integer from 2 to 5.

2-Alkyl Compounds

[0023] Structurally these 2-alkyl analogs are characterized by the general formula VI shown below:

where Y₁, Y₂, R₁₁, R₁₂ and Z are as previously defined herein, and R₁₀ is selected from the group consisting of alkyl, hydroxyalkyl and fluoroalkyl.

Slow Release Compounds

[0024] Modified vitamin D compounds that exhibit a desirable and highly advantageous pattern of biological activity in vivo, namely, the more gradual onset and more prolonged duration of activity, may also be used herein.

[0025] Structurally, the key feature of the modified vitamin D compounds having these desirable biological attributes is that they are derivatives of 17,20(Z)-dehydro-vitamin D analogs, in which a hydrolyzable group is attached to the hydroxy group at carbon 25 and, optionally, to any other of the hydroxy groups present in the molecule. Depending on various structural factors -- e.g. the type, size, structural complexity -- of the attached group, these derivatives hydrolyze to the active 17,20(Z)-dehydro-vitamin D analog, at different rates in vivo, thus providing for the "slow release" of the biologically active vitamin D compound in the body.

[0026] The "slow release" in vivo activity profiles of such compounds can, of course, be further modulated by the use of mixtures of derivatives or the use of mixtures consisting of one or more vitamin D derivative together with underivatized vitamin D compounds.

[0027] It is important to stress that the critical structural feature of the vitamin derivatives identified above is the presence of a hydrolyzable group attached to the hydroxy group at carbon 25 of the molecule. The presence of a hydrolyzable group at that position imparts on the resulting derivatives the desirable "slow-release" biological activity profile mentioned above. Other hydroxy functions occurring in the molecule (e.g. hydroxy functions at carbons 1 or 3) may be present as free hydroxy groups, or one or more of them may also be derivatived with a hydrolyzable group.

[0028] The "hydrolyzable group" present in the above-mentioned derivatives is preferably an acyl group, i.e. a group of the type Q¹CO-, where Q¹ represents hydrogen or a hydrocarbon radical of from 1 to 18 carbons that may be straight chain, cyclic, branched, saturated or unsaturated. Thus, for example, the hydrocarbon radical may be a straight chain or branched alkyl group, or a straight chain or branched alkenyl group with one or more double bonds, or it may be an optionally substituted cycloalkyl or cycloalkenyl group, or an aromatic group, such as substituted or unsubstituted phenyl, benzyl or naphthyl. Especially preferred acyl groups are alkanoyl or alkenoyl groups, of which some typical examples are formyl, acetyl, propanoyl, hexanoyl, isobutyryl, 2-butenoyl, palmitoyl or oleoyl. Another suitable type of hydrolyzable group is the hydrocarbyloxycarbonyl group, i.e. a group of the type Q²-O-CO-, where Q² is a C₁ to C₁₈ hydrocarbon radical as defined above. Exemplary of such hydrocarbon radicals are methyl, ethyl, propyl, and higher straight chain or branched alkyl and alkenyl radicals, as well as aromatic hydrocarbon radicals such as phenyl or benzoyl.

[0029] These modified vitamin D compounds are hydrolyzable in vivo to the active analog over a period of time following administration, and as a consequence regulate the in vivo availability of the active analog, thereby also modulating their activity profile in vivo. The term "activity profile" refers to the biological response over time of vitamin D compounds. Individual modified compounds, or mixtures of such compounds, can be administered to "fine tune" a desired time course of response.

[0030] As used herein the term "modified vitamin D compound" encompasses any vitamin D compound in which one or more of the hydroxy functions present in such a compound are modified by derivatization with a hydrolyzable group. A "hydrolyzable group" is a hydroxy-modifying group that can be hydrolyzed in vivo, so as to regenerate the free hydroxy functions.

[0031] In the context of this disclosure, the term hydrolyzable group preferably includes acyl and hydrocarbyloxycarbonyl groups, i.e. groups of the type Q¹CO- and Q²-O-CO, respectively, where Q¹ and Q² have the meaning defining earlier.

[0032] Structurally, the modified vitamin D compounds encompassed may be represented by the formula VII shown below:

where Y₁, Y₂, R₁₁, R₁₂, R₆, R₇ and Z are as previously defined herein with respect to formula I with the exception that R⁵ in the side chain is -OY₃ and Y₃ is an acyl group or a hydrocarbyloxycarbonyl group, as previously defined herein.

[0033] Some specific examples of such modified vitamin D compounds include 2-substituted derivatives such as:

1,3,25-Triacetates where Y₁=Y₂=Y₃ and is CH₃CO;

1,3,25-Trihexanoates where Y₁=Y₂=Y₃ and is CH₃(CH₂)₄CO;

1,3,25-Trinonanoates where Y₁=Y₂=Y₃ and is CH₃(CH₂)₇CO; and

25-Acetates where Y₁=Y₂ and is H and Y₃ is CH₃CO.

[0034] These compounds can be prepared by known methods. See for example WO97/1105 published March 27, 1999, and the previous description herein.

[0035] 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ (referred to herein as VIT-III) was synthesized and tested. Structurally, this 19-nor analog is characterized by the general formula Ia previously illustrated herein.

[0036] The preparation of 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the structure Ia can be accomplished by the condensation of a bicyclic Windaus-Grundmann type ketone IIa with the allylic phosphine oxide IIIa to the corresponding 17(20)-dehydro-vitamin D analog IVa followed by deprotection at C-1 and C-3 to provide Ia:

In the structures IIIa and IVa, groups Y₁ and Y₂ are hydroxy-protecting groups, preferably t-butyldimethylsilyl, it being also understood that any functionalities that might be sensitive, or that interfere with the condensation reaction, be suitably protected as is well-known in the art. The process shown above represents a specific application of the convergent synthesis concept, which was referred to previously herein and has been applied effectively for the preparation of vitamin D compounds

[0037] The hydrindanone of the general structure IIa is not known. It can be prepared by the method shown in the Schemes herein (see the preparation of compound VIT-III).

[0038] As used in the description and in the claims, the term "hydroxy-protecting group" signifies any group commonly used for the temporary protection of hydroxy functions, such as for example, alkoxycarbonyl, acyl, alkylsilyl or alkylarylsilyl groups (hereinafter referred to simply as "silyl" groups), and alkoxyalkyl groups. Alkoxycarbonyl protecting groups are alkyl-O-CO- groupings such as methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, isopropoxycarbonyl, butoxycarbonyl, isobutoxycarbonyl, tert-butoxycarbonyl, benzyloxycarbonyl or allyloxycarbonyl. The term "acyl" signifies an alkanoyl group of 1 to 6 carbons, in all of its isomeric forms, or a carboxyalkanoyl group of 1 to 6 carbons, such as an oxalyl, malonyl, succinyl, glutaryl group, or an aromatic acyl group such as benzoyl, or a halo, nitro or alkyl substituted benzoyl group. The word "alkyl" as used in the description or the claims, denotes a straight-chain or branched alkyl radical of 1 to 10 carbons, in all its isomeric forms. "Alkoxy" refers to any alkyl radical which is attached by oxygen, i.e. a group represented by "alkyl-o-." Alkoxyalkyl protecting groups are groupings such as methoxymethyl, ethoxymethyl, methoxyethoxymethyl, or tetrahydrofuranyl and tetrahydropyranyl. Preferred silyl-protecting groups are trimethylsilyl, triethylsilyl, t-butyldimethylsilyl, dibutylmethylsilyl, diphenylmethylsilyl, phenyldimethylsilyl, diphenyl-t-butylsilyl and analogous alkylated silyl radicals. The term "aryl" specifies a phenyl-, or an alkyl-, nitro- or halo-substituted phenyl group.

[0039] A "protected hydroxy" group is a hydroxy group derivatised or protected by any of the above groups commonly used for the temporary or permanent protection of hydroxy functions, e.g. the silyl, alkoxyalkyl, acyl or alkoxycarbonyl groups, as previously defined. The terms "hydroxyalkyl", "deuteroalkyl" and "fluoroalkyl" refer to an alkyl radical substituted by one or more hydroxy, deuterium or fluoro groups respectively. An "alkylidene" refers to a radical having the general formula C_kH_2k-where k is an integer.

[0040] More specifically, reference should be made to the following description as well as to the Schemes herein for a detailed illustration of the preparation of compound VIT-III.

SYNTHESIS

[0041] Des-A,B-23,24-dinorcholan-8β,22-diol (2). A flame dried 1000 mL two necked flask was charged with ergocalciferol 1 (5 g, 12.6 mmol), pyridine (5 mL), and anhydrous MeOH (400 mL). The solution was cooled to -78 °C in an argon atmosphere. O₃ was bubbled through the solution until a deep blue color developed and persisted (about 1h). The solution was treated with O₂ until the blue color faded (15 min). Then NaBH₄ (1.5 g, 39.7 mmol) was added. After 15 min. second portion of NaBH₄ (1.5 g, 39.7 mmol) was added and the reaction was allowed to warm to rt. Then the third portion of NaBH₄ (1.5 g, 39.7 mmol) was added and reaction stirred for over night. The reaction was quenched by adding water (50 mL). Methanol was evaporated in vaccuo and residue was dissolved in ethyl acetate. The organic phase was washed with IN aqueous solution of HCl (100 mL), saturated NaHCO₃ solution (100 mL) and brine (100 mL). The organic phase was dried (Na₂SO₄), filtered and evaporated. Purification by silica gel chromatography (25% ethyl acetate/hexane) afforded 2.18 g (10.3 mmol, 81%) of diol 2 as a white solid. Mp 110-111 °C;¹H NMR (400 MHz, CDCl₃) δ: 0.96 (3H, s), 1.03 (3H, d, J = 6.6 Hz), 3.38 (1H, dd, J = 10.5, 6.7 Hz), 3.64 (1H, dd, J = 10.5, 3.2 Hz), 4.09 (1H, m); ¹³C NMR (100 MHz, CDCl₃) δ: 69.2, 67.8, 52.9, 52.4, 41.8, 40.2, 38.2, 33.6, 26.6, 22.6, 17.4, 16.6, 13.6; MS m/z (relative intensity): 212 (M⁺, 2), 194 (M⁺-H₂O, 15), 179 (M⁺-H₂O-CH₃, 18), 125 (43), 111 (100); exact mass calculated for C₁₃H₂₂O [M - H₂O]⁺ is 194.1671, measured is 194.1665.

[0042] Des-A,B-22-(p-toluenesulfonyloxy)-23,24-dinorcholan-8β-ol (3). A solution of diol 2 (1 g, 4.71 mmol) in anhydrous pyridine (12 mL) was cooled to -25 °C and a precooled solution of tosyl chloride (1.08 g, 5.66 mmol) in anhydrous pyridine (2mL) was added dropwise. The reaction mixture was stirred at that temperature for 4 h and allowed to warm to 0 °C and stirred at that temperature for additional 20 h. The mixture was diluted with CH₂Cl₂ (50 mL) and washed with saturated CuSO₄ solution (30 mL), 1N HCl (30 mL), and water (50 mL). The organic phase was dried (Na₂SO₄), filtered and concentrated. Purification by silica gel chromatography (25% ethyl acetate/hexane) yielded 1.7 g (4.64 mmol, 98%) of tosylate 3. ¹H NMR (400 MHz, CDCl₃) δ: 0.89 (3H, s), 0.96 (3H, d, J = 6.6Hz), 2.45 (3H, s), 3.8 (1H, dd, J = 9.2, 6.2 Hz), 3.95 (1H, dd, J = 9.2, 3.0 Hz), 4.06 (1H, m), 7.35 (2H, d, J = 8.2 Hz), 7.78 (2H, d, J = 8.2 Hz);¹³C NMR (100MHz, CDCl₃) δ: 144.7, 133.0, 129.8, 127.9, 75.6, 69.0, 60.4, 52.2, 41.9, 40.1, 35.7, 33.5, 26.4, 22.4, 21.6, 17.3, 16.7, 13.4; MS m/z (relative integration): 366 (M⁺, 6), 194(14), 179(16), 125(30), 111(100); exact mass calculated for C₂₀H₃₀SO₄Na (M + Na⁺) is 389.1763, measured is 389.1768.

[0043] Des-A,B-8β-[(tert-butyldimethylsilyl)oxy]-22-(p-toluenesulfonyloxy)-23,24-dinorcholane (4). To a 0 °C cooled solution of hydroxyl tosylate 3 (1.5 g, 4.09 mmol) in anhydrous DMF (20 mL) was added 2,6-lutidine (0.580 mL, 0.52 g, 4.92 mmol) followed by TBSOTf (1.13 mL, 1.30g, 4.92 mmol). The solution was stirred at 0 °C for 15 min and water (10 mL) was added. The mixture was extracted with ethyl acetate (3 x 40 mL), and combined organic phases were washed with 1N aqueous solution of NaOH (40 mL) dried (Na₂SO₄), filtered and concentrated. The residue was purified by silica gel column chromatography (5% ethyl acetate/hexane) to give 1.94g (4.04 mmol, 99%) of 4. ¹H NMR (400 MHz, CDCl₃) δ: 0.01 (6H, s), 0.88 (12H, s), 0.96 (3H, d, J = 6.8Hz), 2.45 (3H, s), 3.81 (1H, dd, J = 9.2, 6.4Hz), 3.97 (1H, dd, J = 9.7, 3.0Hz), 3.99 (1H, m), 7.34 (2H, d, J = 8.08Hz), 7.79 (2H, d, J = 8.2Hz). ¹³C NMR (100MHz, CDCl₃) δ: 114.5, 133.4, 129.8, 127.9, 74.8, 69.3, 52.3, 52.6, 42.2, 40.5, 35.8, 34.4, 26.6, 25.9, 23.0, 21.6, 18.0, 17.6, 16.8, 13.7, -4.8, -5.1.

[0044] Des-A,B-8β-[(tert-butyldimethylsilyl)oxy]-23,24-dinorcholan-22-al (5). A solution of 4 (1.9 g, 3.96 mmol) in DMSO (5 mL) was added to a suspension of NaHCO₃ (1.5 g, 17.9 mmol) in DMSO (20 mL) at rt. The mixture was heated to 150 °C under argon for 15 min and cooled to rt. Water (50 mL) followed by ethyl acetate (50 mL) were added and aqueous phase was extracted with ethyl acetate (3 x 30 mL). The combined organic phases were dried (Na₂SO₄), filtered and concentrated. The residue was purified by column chromatography (2% ethyl acetate/hexane) to afford 0.93g (2.87 mmol, 76%) of aldehyde 5. H NMR (400 MHz, CDCl₃) δ: 0.01 (6H, 2s), 0.89 (9H, s), 0.97 (3H, s), 1.09 (3H, d, J = 6.8Hz), 2.35 (1H, m), 4.03 (1H, m), 9.58 (1H, d, J = 3.2Hz). ¹³C NMR (100MHz, CDCl₃) δ: 205.2, 69.1, 52.4, 51.8, 49.1, 42.7, 40.5, 30.8, 34.3, 26.2, 25.8, 23.3, 17.6, 14.1, 13.3, -4.7, -5.1.

[0045] Des-A,B-8β-[(tert-butyldimethylsilyl)oxy]-pregnan-20-one (6). A flame dried flask was charged with t-BuOK (1.55 g, 13.9 mmol) and anhydrous t-BuOH (30 mL) at room temperature. O₂ was bubbled through the solution for 15 min. A solution of aldehyde 5 (0.9 g, 2.78 mmol) in anhydrous t-BuOH (15 mL) was added to the reaction mixture and O₂ was bubbled through the solution-for additional 10 min. The reaction was quenched with water (15 mL) and extracted with ether (3 x 30 mL). The combined organic phases were dried (Na₂SO₄), filtered and concentrated. The residue was purified by silica gel column chromatography (3% ethyl acetate/hexane) to give 0.61 g (1.97 mmol, 71%) of the ketone 6. ¹H NMR (400 MHz, CDCl₃) δ: 0.01 (6H, s), 0.84 (3H, s), 0.87 (9H, s), 2.08 (3H, s), 2.46 (1H, t, J = 9.1Hz), 4.03 (1H, m).¹³C NMR (100MHz, CDCl₃) δ: 209.5, 69.0, 64.5, 53.2, 43.7, 39.8, 34.2, 31.6, 25.8, 23.2, 21.8, 17.6, 15.5, -4.8, -5.2.

[0046] 5-Bromo-2-methyl-2-pentanol (8). To a -20 °C cooled solution of ethyl-4-bromobutyrate 7 (5g, 25.6 mmol) in anhydrous diethyl ether (50 mL) was added 3M solution of methylmagnesium bromide in diethyl ether (17.1 mL, 6.11 g, 51.3 mmol) under argon atmosphere over a period of 30 min. The reaction mixture was stirred at room temperature for overnight. Saturated ammonium chloride solution was added to hydrolyze the reaction mixture followed by 1N HCl solution to dissolve the inorganic salts formed. The aqueous phase was extracted with ether (3 x 50mL). The combined extracts were washed with water (100 mL), saturated NaCl solution (100 mL), dried (Na₂SO₄), filtered and concentrated. The residue was purified by silica gel column chromatography (20/80 ethyl acetate/hexane)to afford 3.1g (17.1 mmol, 67%) of tertiary alcohol 8. ¹H NMR (400 MHz, CDCl₃) δ: 1.27 (6H, s), 1.64 (2H, m), 1.96 (2H, m), 3.44 (2H, t, J = 6.68 Hz).

[0047] 5-Bromo-2methyl-2[(tert-butyldimethylsilyl)oxy]-pentane (9). To a -50 °C cooled solution of alcohol 8 (3 g, 16.6 mmol) in anhydrous CH₂Cl₂ (50 mL) was added 2,6-lutidine (2.32 mL, 2.13 g, 19.89 mmol) followed by TBSOTf (4.57 mL, 5.26 g, 19.9 mmol). The solution was stirred at 0 °C for 15 min and water (10 mL) was added. The mixture was extracted with CH₂Cl₂ (3 x 40 mL), and combined organic phases were washed with 1N aqueous solution of NaOH (40 mL), dried (Na₂SO₄), filtered and concentrated. The residue was purified by silica gel column chromatography (1% ethyl acetate/hexane) to give 3.9 g (13.2 mmol, 80%) of 9. ¹H NMR (400 MHz, CDCl₃) δ: 0.07 (6H, s), 0.85 (9H, s), 1.21 (6H, s), 1.55 (2H, m), 1.95 (2H, m), 3.41 (2H, t, J = 6.8Hz)

[0048] Des-A,B-cholest-17(20)-dehydro-8β,25-diols (15a and 15b):

A solution of 5-bromo-2methyl-2[(tert-butyldimethylsilyl)oxy] pentane 9 (2.84g, 9.68 mmol) in anhydrous ether (20 mL, containing catalytic amount of iodine) was added dropwise to a stirred suspension of magnesium powder (0.23 g, 9.68 mmol) in anhydrous diethyl ether (5 mL) at room temperature with occasional warming it up to 35 °C under.argon atmosphere. After generation of the Grignard reagent was complete the mixture was stirred for 1hr at room temperature and for 1hr at 40 °C. Then it was cooled to 0 °C and a solution of ketone 6 (0.6 g, 1.94 mmol) in anhydrous diethyl ether (10 mL) was added dropwise over a period of 30 min. After stirring the reaction mixture at room temperature for 3h it was hydrolyzed with aqueous solution of NH₄Cl (20 mL). The organic layer was separated and aqueous phase was extracted with ethyl acetate (3 x 30 mL). The combined organic phases were washed with water (40 mL), dried (Na₂SO₄) and evaporated. Silica gel column chromatography of the residue gave 0.95 g (94%) of mixture of alcohols 10. Phosphorous oxychloride (3 mL) was added dropwise to a solution of mixture of alcohols 10 (0.95 g) in anhydrous pyridine (20 mL) under argon atmosphere. The reaction was stirred at room temperature overnight and poured into ice-water and extracted with ether (3 x 20 mL). The organic layer was washed with saturated CuSO₄ solution (30 mL), 1N HCl (30 mL), water (50 mL). The organic phase was dried (NaSO₄), filtered and concentrated. Column Chromatography of crude mixture furnished 0.72 g (78%) of mixture of olefins 11a, 11b, 12a, 12b, 13.
The olefin mixture without further purification was dissolved in methanol (20 mL) and p-Toluenesulfonic acid monohydrate (p-TSA) (0.100g) was added at 0 °C. The reaction mixture was stirred at room temperature for 3 days [Additional amounts of p-TSA were successively added (100 mg, 24h; 75 mg, 36h; 50 mg, 48h)]. Methanol was evaporated and residue was diluted with ethyl acetate (30 mL). The organic phase was washed with saturated aqueous NaHCO₃ solution (20 mL) water (20 mL), dried (Na₂CO₃) and evaporated. The residue was purified on column chromatography to yield 0.284 g (79%) of mixture of olefin alcohols 14a, 14b, 15a, 15b, 16. The olefin alcohols were separated on HPLC.

[0049] 17(E)-Des-A,B-cholestan-17(20)-debydro-8β,25-diol (15a). The olefin alcohols were separated on HPLC (9.4 mm x 25 cm zorbax-sil column, 4ml/min) using IPA/hexane (4/96) solvent system. Pure diol 17-20E 15a 70 mg (250 µmol, 25%) was eluted at Rv = 50 mL. [α]²²_D -16.5° (c 1.02, CHCl₃); ¹H NMR (400 MHz, CDCl₃) δ: 1.09 (3H, s), 1.20 (6H, s), 1.67 (3H, t, J = 1.84Hz), 4.14 (1H, m). ¹³C NMR (100MHz, CDCl₃) δ: 143.2, 123.7, 71.0, 69.8, 52.4, 43.9, 43.7, 38.3, 36.8, 33.4, 29.2, 28.5, 23.5, 22.2, 19.1, 17.9, 17.2. MS m/z (relative intensity): 280 (M⁺, 16), 262 (M-H₂O⁺, 7), 229 (M-2xH₂O -CH₃⁺, 16) 179(54), 161(100); Exact mass calculated for C₁₈H₃₂O₂ [M+Na]⁺ is 303.2300, found 303.2297.

[0050] 17(E)-25-(Triethylsilyloxy)-des-A,P-cholestan-17(20)-dehydro-8-one (17a). To a solution of alcohol 15a (20 mg, 71 µmol) in anhydrous CH₂Cl₂ (5 mL) was added PDC (40 mg, 107 µmol) at rt. After stirring the reaction for 3 h under argon atmosphere the solution was passed through a pad of celite with ethyl acetate. The filtrate was concentrated and applied on a Sep-Pak cartridge and eluted with ethyl acetate/hexane (20/80) to give 17 mg, (61.1 µmol, 86%) of ketone as colorless oil. To a -50 °C cooled solution of ketone (17 mg, 61.1 µmol) in anhydrous CH₂Cl₂ (5 mL) was added 2,6 lutidine 9 µL, 7.86 mg, 13.3 µmol) followed by TESOTf (17 µL, 19.4 mg, 73.3 µmol). The solution was stirred at 0 °C for 15 min and water (5 mL) was added. The mixture was extracted with CH₂ Cl₂ (3 x 5 mL), and combined organic phases were dried (Na₂SO₄), filtered and concentrated. The ketone was purified on HPLC (9.4-mm x 25-cm Zorbax-Sil column, 4ml/min) using 10% ethyl acetate/hexane solvent system. Pure ketone 17a 14.4 mg (36.7 µmol, 60%) was eluted at R_v = 20 mL as colorless oil. [α]²⁵_D-14.4 (c 0.73, CHCl₃); ¹H NMR (400 MHz, CDCl₃) δ: 0.56 (6H, q, J = 7.7Hz), 0.84 (3H, s), 0.94 (9H, t, J = 4.76Hz), 1.18 (6H, s), 1.71 (3H, t, J = 1.84Hz), 2.57 (1H, dd, J = 12, 6.2 Hz). ¹³C NMR (100MHz, CDCl₃) δ: 212.2, 141.2, 126.1, 73.3, 61.8, 50.5, 44.7, 40.6, 36.9, 36.7, 29.9, 29.8, 28.7, 23.9, 22.1, 20.2, 17.8, 17.6, 7.1, 6.8. MS m/z (relative intensity): No M⁺, 377([M-CH₃]⁺, 3) 363([M-C₂H₅]⁺, 9), 204(100), 189((18), 161(45). Exact mass calculated for C₂₄H₄₄O₂Si [M+Na]⁺ is 415.3008, found 415.3016.

[0051] 17(E)-1α,25 Dihydroxy-17(20)-dehydro-2-methylene-19-norvitamin D₃ (20a). To a solution of phosphine oxide 18 (0.051 g, 87.6 µmol) in anhydrous THF (500 µL) at - 25 °C was slowly added PhLi 1.2M in cyclohexane/ether (70/30) (80 µL, 8.1 mg, 96.4 µmol) under argon with stirring. The solution turned deep orange. The mixture was stirred at that temperature for 20 min and cooled to -78 °C. A precooled (-78 °C) solution of ketone 17a (14 mg, 35.7 µmol) in anhydrous THF (100 µL) was added slowly. The mixture was stirred under argon atmosphere at -78 °C for 3h and at 0 °C for 18h. Ethyl acetate was added and organic phase was washed with brine, dried (Na₂SO₄) and evaporated. The residue was applied on a Sep-Pak cartridge, and eluted with 1% ethyl acetate/hexane to give 19-nor protected vitamin derivative 19a (8 mg of unreacted ketone 17a was recovered). The protected vitamin was further purified by HPLC (9.4-mm x 25-cm Zorbax-Sil column, 4ml/min) using hexane/IPA (99.95/0.05) solvent system. Pure compound 19a, 7.7mg (10.2 µmol, 29%) was eluted at R_v= 20 mL as color less oil. UV (in-hexane) λ_max 243.1, 252, 262.2 nm; ¹H NMR (400 MHz, CDCl₃) δ: 0.03, 0.05, 0.07, 0.08 (each 3H, each s), 0.56 (6H, q, J = 7.8 Hz), 0.74 (3H, s), 0.87 and 0.91 (each 9H, each s), 0.96 (9H, t, J=7.8 Hz), 1.19 (6H, s), 1.68(3.H, t, J = 1.86 Hz), 2.18 (1H, dd, J = 12.6, 8.3 Hz), 2.33 (1H, m) 2.46 (1H, dd, 12.6, 4.6 Hz), 2.53 (1H, dd, 13.3, 5.88 Hz), 2.80 (1H, m), 4.43 (2H, m), 4.93 and 4.97 (1H and 1H, each s), 5.88 and 6.21 (1H and 1H, each d, J = 11.2 Hz); MS m/z (relative intensity): No M⁺, 624(59), 366(32), 91(100); Exact mass calculated for C₄₅H₈₄O₃Si₃ [M+Na]⁺ is 779.5626, found 779.5648.
The protected vitamin 19a (7.7 mg, 10.2 µmol) was dissolved in anhydrous THF (500 µL) and treated with TBAF (0.102 mL, 26.7 mg, 102 µmol) and stirred at rt in dark for overnight. The solvent was removed in vaccuo and residue was applied on Sep-Pak cartridge, and eluted with 30% ethyl acetate/hexane to get the deprotected vitamin 20a. The vitamin was further purified by HPLC (9.4-mm x 25-cm Zorbax-Sil column, 3 mL/min) using hexane/IPA (90/10) as solvent system. Pure vitamin 20a, 2.9 mg (7 µmol, 69%) was collected at R_v = 42 mL as white solid: UV (in EtOH) λ_mas 242.9, 251, 261.2 nm; ¹H NMR (500 MHz, CDCl₃) δ: 0.74 (3H, s), 1.22 (6H, s), 1.69 (3H, t, J = 1.94 Hz,), 2.29 (1H, dd, J = 13.0, 8.39 Hz), 2.32 (1H, dd, J = 13.9, 7.0 Hz), 2.57 (1H, dd, J = 13.4, 3.49 Hz), 2.79 (1H, br d) 2.87(1H, dd, J = 13.0, 4.59 Hz), 4.49 (2H, m), 5.09 and 5.11 (1H and 1H, each s), 5.92 and 6.35 (1H and 1H, each d, J = 11.29 Hz); MS m/z (relative intensity): 414 (M⁺, 36), 396([M-H₂O]⁺, 6), 381([M-H₂O-CH₃]⁺, 8) 285(70), 149(61), 69(100).

[0052] 17(Z)-Des-A,B-cholest-17(20)-dehydro-8β,25-diol (15b). The olefin alcohols were separated on HPLC (9.4mm x 25cm zorbax-sil column, 4ml/min) using IPA/hexane (5/95) solvent system. Diol 17-20Z 15b and Diol 20-21 16 eluted out together at Rv = 45 mL. The alcohols were oxidized together.

[0053] 17(Z)-25-(Triethylsityloxy)-des-A,B-cholest-17(20)-dehydro-8-one (17b). To a solution of mixture of alcohols 15b and 16 (34 mg, 121 µmol) in anhydrous CH₂Cl₂ (5 mL) was added PDC (55 mg, 145.7 µmol) at rt. After stirring the reaction for 3h under argon atmosphere the solution was passed through a pad of celite with ethyl acetate.
The filtrate was concentrated and applied on a Sep-Pak cartridge and eluted with ethyl acetate/hexane (20/80) to give a mixture of ketones 17b and 16b 30.2 mg (108.6 µmol, 89%) as colorless oil. To a -50 °C cooled solution of ketones 30.2 mg (30.2 mg, 108.6 µmol) in anhydrous CH₂Cl₂ (10 mL) was added 2,6-lutidine (16 µL, 13.9 mg, 130.3 µmol) followed by TESOTf (30 µL, 34.5 mg, 130.3 µmol). The solution was stirred at 0 °C for 15 min and water (10 mL) was added. The mixture was extracted with CH₂Cl₂ (3 x 5 mL), and combined organic phases dried (Na₂SO₄), filtered and concentrated. The residue was purified by HPLC (9.4-mm x 25-cm Zorbax-Sil column, 4 ml/min) using ethyl acetate/hexane (5/95) solvent system. Pure ketone 17b 7.7 mg (19.6 µmol, 18%) was eluted at R_v = 34mL as colorless oil. ¹H NMR (400.13 MHz, CDCl₃) δ: 0.56 (6H, q, J = 7.78 Hz), 0.83 (3H, s), 0.94 (9H, t, J = 7.9 Hz), 1.2 (6H, s), 1.57 (3H, br s), 2.57 (1H, dd, J = 11.8, 6.3 Hz); ¹³CNMR (100MHz, CDCl₃) δ: 212.18, 141.1, 126.8, 73.2, 62.0, 50.5, 45.3, 40.7, 37.1, 34.5, 29.9, 29.8, 24.0, 23.8, 20.2, 20.1, 18.7, 7.1, 6.8. MS m/z (relative intensity): No M⁺, 363 ([M - C₂H₅]⁺, 10), 334 ([M - 2xC₂H₅]⁺, 1), 204 (100).

[0054] 17(Z)-1α,25 Dihydroxy-17(20)-dehydro-2-methylene-19-norvitamin D₃ (20b). To a solution of phosphine oxide 10 (62 mg, 106.5 µmol) in anhydrous THF (750 µL) at - 25 °C was slowly added PhLi 1.8 M in Di-n-butyl ether (59 µL, 8.9mg, 106.5 µmol) under argon with stirring. The solution turned deep orange. The mixture was stirred at that temperature for 20 min and cooled to -78 °C. A precooled (-78 °C) solution of ketone 17b (7.7 mg, 19.6 µmol) in anhydrous THF (100 µL) was added slowly. The mixture was stirred under argon atmosphere at -78 °C for 3h and at 0 °C for 18h. Ethyl acetate was added and organic phase was washed with brine, dried-(Na₂SO₄) and evaporated. The residue was applied on a Sep-Pak cartridge, and eluted with 1% ethyl acetate/hexane to give the 19-nor protected vitamin derivative. The vitamin was further purified by HPLC (9.4-mm x 25-cm Zorbax-Sil column, 4ml/min) using hexane/IPA (99.95:0.05) solvent system. Pure compound 19b, 12.8 mg (16.9 µmol, 86%) was eluted at R_v= 19 mL as colorless oil. [α]²⁵_D -9.35 (c 0.64, CHCl₃); UV (in hexane): λ_max 244.4, 253.2, 263.2 nm; ¹H NMR (400 MHz, CDCl₃) δ: 0.026, 0.050, 0.067, 0.082 (each 3H, each s), 0.56 (6H, q, J = 7.84 Hz), 0.74 (3H, s), 0.86, 0.89 (each 9H, each s), 0.94 (9H, t, J = 7.96 Hz), 1.19 (6H, s), 1.56 (3H, br s), 2.14 (1H, dd, J = 12.5, 4.8 Hz), 2.33 (1H, dd, J = 13.1, 2.8 Hz), 2.46 (1H, dd, J = 12.7, 4.4 Hz), 2.53 (1H, dd, J = 13.3, 6.0 Hz), 2.80 (1H, br d, J = 13.5 Hz), 4.43 (2H, m), 4.92 and 4.97 (each 1H, each s), 5.88 and 6.21 (each 1H, each d, J = 11. 1Hz); ¹³C NMR (100 MHz, CDCl₃) δ: 152.9, 142.2, 140.8, 132.8, 125.9, 122.3, 116.3, 106.2, 73.3, 72.5, 71.6, 56.7, 47.6, 46.8, 45.4, 30.1, 29.8, 28.5, 25.8, 25.7, 23.9, 23.6, 23.0, 19.9, 18.2, 18.1, 17.8, 7.1, 6.8, -4.8, -5.0; MS m/z (relative intensity): No M⁺, 366(2), 263(100); Exact mass calculated for C₄₅H₈₄O₃Si₃[M+Na]⁺ is 779.5624, found 779.5647.
The protected vitamin 19b (12.8 mg, 16.9 mmol) was dissolved in anhydrous THF (500 µL) and treated with TBAF (170 µL, 44.2 mg, 169 µmol) and stirred at rt in dark for overnight. The solvent was removed in vaccuo and residue was applied on Sep-Pak cartridge, and eluted with 30% ethyl acetate/hexane to get the deprotected vitamin. The vitamin was further purified by HPLC (9.4-mm x 25-cm Zorbax-Sil column, 4ml/min) using hexane/IPA (85/15) as solvent system. Pure vitamin 20b, 4.3mg (10.3 µmol, 62%) was eluted at R_v = 33 mL. UV (in ethanol): λ_max 244.1, 252.5, 262.1nm; ¹H NMR (400 MHz, CDCl₃) δ: 0.74 (3H, s), 1.21 (6H, s), 1.57 (3H, br s), 2.30 (2H, m), 2.57 (1H, dd, J = 13.3, 3.6 Hz), 2.79 (1H, dd, J = 11.6,2.52 Hz), 2.85 (1H, dd, J = 13.1, 4.44Hz), 4.48 (2H, m), 5.09 and 5.11 (1H and 1H, each s), 5.92 and 6.35 (1H and 1H, each d, J = 11.3 Hz); MS m/z (relative intensity):414 (M⁺, 90), 399 (M-CH₃⁺, 17), 381 [M-CH₃-H₂O]⁺, 18), 363 ([M-CH₃-2 x H₂O]⁺, 7), 285 (86), 243 (35), 91 (100); exact mass calculated for C₂₇H₄₂O₃ ([M+Na]⁺) is 437.3032, measured is 437.3026.

SCHEMES

[0055] 

BIOLOGICAL ACTIVITY OF 17(Z)-1α,25-DIHYDROXY-17(20)-DEHYDRO-2-METHYLENE-19-NORVITAMIN D₃

[0056] The introduction of a methylene group to the 2-position, the introduction of a double bond between the 17 and 20 positions, and orienting the side chain of 1α,25-dihydroxy-19-nor-vitamin D₃ in its Z configuration had little or no effect on binding to the full length recombinant rat vitamin D receptor, as compared to 1α,25-dihydroxyvitamin D₃. The compound VIT-III bound equally well to the receptor as compared to the standard 1,25-(OH)₂D₃ (Figure 1). It might be expected from these results that compound VIT-III would have equivalent biological activity. Surprisingly, however, compound VIT-III is a highly selective analog with unique biological activity.

[0057] Figure 5 shows that VIT-III has relatively high activity as compared to that of 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃), the natural hormone, in stimulating intestinal calcium transport.

[0058] Figure 4 demonstrates that VIT-III has relatively high bone calcium mobilization activity, as compared to 1,25(OH)₂D_3.

[0059] Figures 4-5 thus illustrate that VIT-III may be characterized as having relatively high, calcemic activity. Their preferential activity on intestinal calcium transport and calcium mobilizing activity allows the in vivo administration of these compounds for the treatment and prophylaxis of metabolic bone diseases. Because of their preferential calcemic activity on gut calcium transport and on bone, these compounds would be preferred therapeutic agents for the treatment and prophylaxis of diseases such as osteoporosis, especially low bone turnover osteoporosis, steroid induced osteoporosis, senile osteoporosis or postmenopausal osteoporosis, as well as osteomalacia and renal osteodystrophy.

[0060] Figure 2 illustrates that VIT-III is as potent as 1,25(OH)₂D₃ on HL-60 cell differentiation, making it an excellent candidate for the treatment of psoriasis and cancer, especially against leukemia, colon cancer, breast cancer, skin cancer and prostate cancer.
In addition, due to its relatively high cell differentiation activity, this compound provides a therapeutic agent for the treatment of various skin conditions including wrinkles, lack of adequate dermal hydration, i.e. dry skin, lack of adequate skin firmness, i.e. slack skin, and insufficient sebum secretion. Use of this compound thus not only results in moisturizing of skin but also improves the barrier function of skin.

[0061] Figure 3 illustrates that the compound VIT-III has about the same transcriptional activity as 1α,25-dihydroxyvitamin D₃ in bone cells. This result, together with the cell differentiation activity of Figure 2, suggests that VIT-III will be very effective in psoriasis because it has direct cellular activity in causing cell differentiation and in suppressing cell growth. These data also indicate that VIT-III may have significant activity as an anti-cancer agent, especially against leukemia, colon cancer, breast cancer, skin cancer and prostate cancer, as well as a therapy for treating and/or preventing obesity.

[0062] The strong activity of VIT-III on HL-60 differentiation suggests it will be active in suppressing growth of parathyroid glands and in the suppression of the preproparathyroid gene. This analog having relatively high calcemic activity is also expected to be useful as a therapy to treat hypoparathyroidism since it is effective to raise blood calcium levels.

EXPERIMENTAL METHODS

[0063] The compounds of the invention were prepared and studied using the following methods.

Vitamin D Receptor Binding

Test Material

Protein Source

[0064] Full-length recombinant rat receptor was expressed in E. coli BL21 (DE3) Codon Plus RIL cells and purified to homogeneity using two different column chromatography systems. The first system was a nickel affinity resin that utilizes the C-terminal histidine tag on this protein. The protein that was eluted from this resin was further purified using ion exchange chromatography (S-Sepharose Fast Flow). Aliquots of the purified protein were quick frozen in liquid nitrogen and stored at -80°C until use. For use in binding assays, the protein was diluted in TEDK₅₀ (50 mM Tris, 1.5 mM EDTA, p7.4, 5 mM DTT, 150 mM KCI) with 0.1 % Chaps detergent. The receptor protein and ligand concentration was optimized such that no more than 20% of the added radiolabeled ligand was bound to the receptor.

Study Drugs

[0065] Unlabeled ligands were dissolved in ethanol and the concentrations determined using UV spectrophotometry (1,25(OH)₂D₃: molar extinction coefficient = 18,200 and λ_max = 265 nm). Radiolabeled ligand (³H-1,25(OH)₂D₃, ∼159 Ci/mmole) was added in ethanol at a final concentration of 1 nM.

Assay Conditions

[0066] Radiolabeled and unlabeled ligands were added to 100 mcl of the diluted protein at a final ethanol concentration of ≤10%, mixed and incubated overnight on ice to reach binding equilibrium. The following day, 100 mcl of hydroxylapatite slurry (50%) was added to each tube and mixed at 10-minute intervals for 30 minutes. The hydroxylapaptite was collected by centrifugation and then washed three times with Tris-EDTA buffer (50 mM Tris, 1.5 mM EDTA, pH 7.4) containing 0.5% Titron X-100. After the final wash, the pellets were transferred to scintillation vials containing 4 ml of Biosafe II scintillation cocktail, mixed and placed in a scintillation counter. Total binding was determined from the tubes containing only Radiolabeled ligand.

HL-60 Differentiation

Test Material

Study Drugs

[0067] The study drugs were dissolved in ethanol and the concentrations determined using UV spectrophotometry. Serial dilutions were prepared so that a range of drug concentrations could be tested without changing the final concentration of ethanol (≤ 0.2%) present in the cell cultures.

Cells

[0068] Human promyelocytic leukemia (HL60) cells were grown in RPMI-1640 medium containing 10% fetal bovine serum. The cells were incubated at 37°C in the presence of 5% CO₂.

Assay Conditions

[0069] HL60 cells were plated at 1.2 x 10⁵ cells/ml. Eighteen hours after plating, cells in duplicate were treated with drug. Four days later, the cells were harvested and a nitro blue tetrazolium reduction assay was performed (Collins et al., 1979; J. Exp. Med. 149:969-974). The percentage of differentiated cells was determined by counting a total of 200 cells and recording the number that contained intracellular black-blue formazan deposits. Verification of differentiation to monocytic cells was determined by measuring phagocytic activity (data not shown).

In vitro Transcription Assay

[0070] Transcription activity was measured in ROS 17/2.8 (bone) cells that were stably transfected with a 24-hydroxylase (24Ohase) gene promoter upstream of a luciferase reporter gene (Arbour et al., 1998). Cells were given a range of doses. Sixteen hours after dosing the cells were harvested and luciferase activities were measured using a luminometer. RLU = relative luciferase units.

[0071] Antagonism was tested by adding a combination of 1,25(OH)₂D₃ and the compound in the same well keeping the final ethanol concentration the same.

Intestinal Calcium Transport and Bone Calcium Mobilization

[0072] Male, weanling Sprague-Dawley rats were placed on Diet 11 (Suda et al, J. Nutr. 100:1049, 1970) (0.47% Ca) + vitamins AEK for one week followed by Diet 11 (0.02% Ca) + vitamins AEK for 3 weeks. The rats were then switched to the same diet containing 0.47% Ca for one week followed by two weeks on the same diet containing 0.02% Ca. Dose administration began during the last week on 0.02% calcium diet. Four consecutive ip doses were given approximately 24 hours apart. Twenty-four hours after the last dose, blood was collected from the severed neck and the concentration of serum calcium determined by atomic absorption spectrometry as a measure of bone calcium mobilization. The first 10 cm of the intestine was also collected for intestinal calcium transport analysis using the everted gut sac method.

[0073] Antagonism was tested by administering a combination of 1,25(OH)₂D₃ and the compound to the animal simultaneously.

INTERPRETATION OF DATA

[0074] VDR binding, HL60 cell differentiation, and transcription activity. VIT-III (K_¡=5.0x10^-11M) is equivalent to the natural hormone 1α,25-dihydroxyvitamin D₃ (K_i=4.9x10^-11M) in its ability to compete with [³H]-1,25(OH)₂D₃ for binding to the full-length recombinant rat vitamin D receptor (Figure 1). VIT-III is also significantly higher (EC₅₀=3.7x10^-10M) in its ability (efficacy or potency) to promote HL60 differentiation as compared to 1α,25-dihydroxyvitamin D₃ (EC₅₀=1.3x10^-9M) (See Figure 2). Also, compound VIT-III (EC₅₀=4.4x10^-12M) has greater transcriptional activity in bone cells than 1α,25-dihydroxyvitamin D₃ (EC₅₀=2.9x10^-10M) (See Figure 3). These results suggest that VIT-III will be very effective in psoriasis because it has direct cellular activity in causing cell differentiation and in suppressing cell growth. These data also indicate that VIT-III will have significant activity as an anti-cancer agent, especially against leukemia, colon cancer, breast cancer, skin cancer and prostate cancer, as well as against skin conditions such as dry skin (lack of dermal hydration), undue skin slackness (insufficient skin firmness), insufficient sebum secretion and wrinkles. It would also be expected to be very active in suppressing secondary hyperparathyroidism.

[0075] Calcium mobilization from bone and intestinal calcium absorption in vitamin D-deficient animals. Using vitamin D-deficient rats on a low calcium diet (0.02%), the activities of VIT-III and 1,25(OH)₂D₃ in intestine and bone were tested. As expected, the native hormone (1,25(OH)₂D₃) increased serum calcium levels at the dosage tested (Fig. 4). Figure 4 also shows that VIT-III has significantly greater activity in mobilizing calcium from bone than 1,25(OH)₂D₃. Administration of VIT-III at 260 pmol/day for 4 consecutive days resulted in significant mobilization of bone calcium, and increasing the amount of VIT-III to 2340 pmol/day and then to 7020 pmol/day also increased the effect to provide progressively increasing serum calcium levels well above the effects seen with 1,25(OH)₂D₃.

[0076] Intestinal calcium transport was evaluated in the same groups of animals using the everted gut sac method (Figure 5). These results show that the compound VIT-II1 promotes a significant increase in intestinal calcium transport activity when administered at 260 pmol/day, 2340 pmol/day or 7020 pmol/day, as compared to 1,25(OH)₂D₃ at the 260 pmol/day dose. Thus, it may be concluded that VIT-III has significant intestinal calcium transport activity at the tested doses.

[0077] These results illustrate that VIT-III is an excellent candidate for numerous human therapies as described herein, and that it may be particularly useful in a number of circumstances such as suppression of secondary hyperparathyroidism of renal osteodystrophy, autoimmune diseases, cancer, and psoriasis. VIT-III is an excellent candidate for treating psoriasis because: (1) it has significant VDR binding, transcription activity and cellular differentiation activity; (2) it is devoid of hypercalcemic liability unlike 1,25(OH)₂D₃; and (3) it is easily synthesized. Since VIT-III has significant binding activity to the vitamin D receptor, but has little ability to raise blood serum calcium, it may also be particularly useful for the treatment of secondary hyperparathyroidism of renal osteodystrophy.

[0078] These data also indicate that the compound VIT-III of the invention may be especially suited for treatment and prophylaxis of human disorders which are characterized by an imbalance in the immune system, e.g. in autoimmune diseases, including multiple sclerosis, lupus, diabetes mellitus, host versus graft rejection, and rejection of organ transplants; and additionally for the treatment of inflammatory diseases, such as rheumatoid arthritis, asthma, and inflammatory bowel diseases such as celiac disease, ulcerative colitis and Crohn's disease. Acne, alopecia and hypertension are other conditions which may be treated with the compound VIT-III of the invention.

[0079] The compounds of the invention of formula I, and particularly formula Ia, are also useful in preventing or treating obesity, inhibiting adipocyte differentiations, inhibiting SCD-1 gene transcription, and/or reducing body fat in animal subjects. Therefore, in some embodiments, a method of preventing or treating obesity, inhibiting adipocyte differentiations, inhibiting SCD-1 gene transcription, and/or reducing body fat in an animal subject includes administering to the animal subject, an effective amount of one or more of the compounds or a pharmaceutical composition that includes one or more of the compounds of formula I. Administration of one or more of the compounds or the pharmaceutical compositions to the subject inhibits adipocyte differentiation, inhibits gene transcription, and/or reduces body fat in the animal subject. The animal may be a human, a domestic animal such as a dog or a cat, or an agricultural animal, especially those that provide meat for human consumption, such as fowl like chickens, turkeys, pheasant or quail, as well as bovine, ovine, caprine, or porcine animals.

[0080] For prevention and/or treatment purposes, the compounds of this invention defined by formula I and Ia may be formulated for pharmaceutical applications as a solution in innocuous solvents, or as an emulsion, suspension or dispersion in suitable solvents or carriers, or as pills, tablets or capsules, together with solid carriers, according to conventional methods known in the art. Any such formulations may also contain other pharmaceutically acceptable and non-toxic excipients such as stabilizers, anti-oxidants, binders, coloring agents or emulsifying or taste-modifying agents.

[0081] The compounds of formula I and particularly VIT-III of formula Ia, may be administered orally, topically, parenterally, rectally, nasally, sublingually, or transdermally. The compound is advantageously administered by injection or by intravenous infusion or suitable sterile solutions, or in the form of liquid or solid doses via the alimentary canal, or in the form of creams, ointments, patches, or similar vehicles suitable for transdermal applications. A dose of from 0.01µg to 1000µg per day of the compounds I, particularly VIT-III, preferably from about 0.1µg to about 500 µg per day, is appropriate for prevention and/or treatment purposes, such dose being adjusted according to the disease to be treated, its severity and the response of the subject as is well understood in the art. Since the compound exhibits specificity of action, each may be suitably administered alone, or together with graded doses of another active vitamin D compound -- e.g. 1α-hydroxyvitamin D₂ or D₃, or 1α,25-dihydroxyvitamin D₃ -- in situations where different degrees of bone mineral mobilization and calcium transport stimulation is found to be advantageous.

[0082] Compositions for use in the above-mentioned treatments comprise an effective amount of the compounds I, particularly VIT-III, as defined by the above formula I and Ia as the active ingredient, and a suitable carrier. An effective amount of such compound for use in accordance with this invention is from about 0.01 µg to about 1000 µg per gm of composition, preferably from about 0.1 µg to about 500 µg per gram of composition, and may be administered topically, transdermally, orally, rectally, nasally, sublingually or parenterally in dosages of from about 0.01µg/day to about 1000 µg/day, and preferably from about 0.1 µg/day to about 500 µg/day.

[0083] The compounds I, particularly VIT-III, may be formulated as creams, lotions, ointments, topical patches, pills, capsules or tablets, suppositories, aerosols, or in liquid form as solutions, emulsions, dispersions, or suspensions in pharmaceutically innocuous and acceptable solvent or oils, and such preparations may contain in addition other pharmaceutically innocuous or beneficial components, such as stabilizers, antioxidants, emulsifiers, coloring agents, binders or taste-modifying agents.

[0084] The compounds I, particularly VIT-III, may be advantageously administered in amounts sufficient to effect the differentiation of promyelocytes to normal macrophages. Dosages as described above are suitable, it being understood that the amounts given are to be adjusted in accordance with the severity of the disease, and the condition and response of the subject as is well understood in the art.

[0085] The formulations of the present invention comprise an active ingredient in association with a pharmaceutically acceptable carrier therefore and optionally other therapeutic ingredients. The carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulations and not deleterious to the recipient thereof.

[0086] Formulations of the present invention suitable for oral administration may be in the form of discrete units as capsules, sachets, tablets or lozenges, each containing a predetermined amount of the active ingredient; in the form of a powder or granules; in the form of a solution or a suspension in an aqueous liquid or non-aqueous liquid; or in the form of an oil-in-water emulsion or a water-in-oil emulsion.

[0087] Formulations for rectal administration may be in the form of a suppository incorporating the active ingredient and carrier such as cocoa butter, or in the form of an enema.

[0088] Formulations suitable for parenteral administration conveniently comprise a sterile oily or aqueous preparation of the active ingredient which is preferably isotonic with the blood of the recipient.

[0089] Formulations suitable for topical administration include liquid or semi-liquid preparations such as liniments, lotions, applicants, oil-in-water or water-in-oil emulsions such as creams, ointments or pastes; or solutions or suspensions such as drops; or as sprays.

[0090] For nasal administration, inhalation of powder, self-propelling or spray formulations, dispensed with a spray can, a nebulizer or an atomizer can be used. The formulations, when dispensed, preferably have a particle size in the range of 10 to 100µ.

[0091] The formulations may conveniently be presented in dosage unit form and may be prepared by any of the methods well known in the art of pharmacy. By the term "dosage unit" is meant a unitary, i.e. a single dose which is capable of being administered to a patient as a physically and chemically stable unit dose comprising either the active ingredient as such or a mixture of it with solid or liquid pharmaceutical diluents or carriers.

Claims

1. A compound having the formula:

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group -(CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and -CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

2. The compound of claim 1 wherein Y₂ is hydrogen.

3. The compound of claim 1 wherein Y₁ is hydrogen.

4. The compound of claim 1 wherein Y₁, Y₂ and R⁵ are hydrogen.

5. A compound according to claim 1, which is a 17,20(Z)-dehydro vitamin D analog having the formula:

where Y₁ and Y₂, which may be the same or different, are each selected from hydrogen or a hydroxy-protecting group.

6. The compound of claim 5, wherein Y₂ is hydrogen.

7. The compound of claim 5, wherein Y₁ is hydrogen.

8. The compound of claim 5, wherein Y₁ and Y₂ are both t-butyldimethylsilyl.

9. A compound according to claim 1, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the formula:

10. A pharmaceutical composition containing an effective amount of at least one compound according to claims 1, 5 or 9, together with a pharmaceutically acceptable excipient.

11. The pharmaceutical composition of claim 10, wherein said effective amount comprises from 0.01 µg to 1000 µg per gram of composition, preferably from 0.1 µg to 500 µg per gram of composition.

12. A compound according to claim 1 which is a 17(20)-dehydro vitamin D analog of the following formula for use in treating psoriasis,

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group -(CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and -CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R ¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

13. A compound according to claim 9, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the following formula for use in treating psoriasis.

14. A compound according to claim 1, which is 17(20)-dehydro vitamin D analog of the following formula for use in treating a disease selected from the group consisting of leukemia, colon cancer, breast cancer, skin cancer or prostate cancer

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group -(CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and -CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R ¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-,
where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

15. A compound according to claim 9, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the formula for use in treating a disease selected from the group consisting of leukemia, colon cancer, breast cancer, skin cancer or prostate cancer.

16. A compound according to claim 1, which is a 17(20)-dehydro vitamin D analog of the following formula for use in treating an autoimmune disease selected from the group consisting of multiple sclerosis, lupus, diabetes mellitus, hot versus graft rejection, and rejection of organ transplants

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group -(CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and -CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

17. A compound according to claim 9, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the following formula for use in treating an autoimmune disease selected from the group consisting of multiple sclerosis, lupus, diabetes mellitus, host versus graft rejection, and rejection of organ transplants.

18. A compound according to claim 1, which is a 17(20)-dehydro vitamin D analog of the following formula for use in treating an inflammatory disease selected from the group consisting of rheumatoid arthritis, asthma, and inflammatory bowel diseases,

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group - (CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and
-CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

19. A compound according to claim 9, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the following formula for use in treating an inflammatory disease selected from the group consisting of rheumatoid arthritis, asthma, and inflammatory bowel diseases.

20. A compound according to claim 1, which is a 17(20)-dehydro vitamin D analog of the following formula for use in treating a skin condition selected from the group consisting of wrinkles, lack of adequate skin firmness, lack of adequate dermal hydration and insufficient sebum secretion

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group -(CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and -CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R ¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

21. A compound according to claim 9, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the following formula for use in treating a skin condition selected from the group consisting of wrinkles, lack of adequate skin firmness, lack of adequate dermal hydration and insufficient sebum secretion.

22. A compound according to claim 1, which is a 17(20)-dehydro vitamin D analog of the following formula for use in treating renal osteodystrophy

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group -(CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and
-CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

23. A compound according to claim 9, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the formula for use in treating renal osteodystrophy.

24. A compound according to claim 1, which is a 17(20)-dehydro vitamin D analog of the following formula for use in treating or preventing obesity of an animal, inhibiting adipocyte differentiation, inhibiting SCD-1 gene transcription, and/or reducing body fat in an animal

where Y₁ and Y₂, which may be the same or different, are each selected from the group consisting of hydrogen and a hydroxy-protecting group, where R₁₁ and R₁₂ are each hydrogen, where R₆ and R₇, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or R₆ and R₇ when taken together may represent the group - (CH₂)_x- where x is an integer from 2 to 5, or R₆ and R₇ when taken together may represent the group =CR₈R₉ where R₈ and R₉, which may be the same or different, are each selected from the group consisting of hydrogen, alkyl, hydroxyalkyl, fluoroalkyl, hydroxy and alkoxy, or when taken together R₈ and R₉ may represent the group -(CH₂)_x- where x is an integer from 2 to 5, and where the group R represents a side chain represented by the structure

where the side chain and 17-ene double bond is in the Z configuration and where Z in the above side chain structure is selected from Y, -OY, -CH₂OY, -C≡CY and
-CH=CHY, where the double bond in the side chain may have the cis or trans geometry, and where Y is selected from hydrogen, methyl, -COR⁵ and a radical of the structure:

where m and n, independently, represent the integers from 0 to 5, where R¹ is selected from hydrogen, deuterium, hydroxy, protected hydroxy, fluoro, trifluoromethyl, and C_1-5-alkyl, which may be straight chain or branched and, optionally, bear a hydroxy or protected-hydroxy substituent, and where each of R², R³, and R⁴, independently, is selected from deuterium, deuteroalkyl, hydrogen, fluoro, trifluoromethyl and C_1-5 alkyl, which may be straight-chain or branched, and optionally, bear a hydroxy or protected-hydroxy substituent, and where R¹ and R², taken together, represent an oxo group, or an alkylidene group having a general formula C_kH_2k- where k is an integer, the group =CR²R³, or the group -(CH₂)_p-, where p is an integer from 2 to 5, and where R³ and R⁴, taken together, represent an oxo group, or the group -(CH₂)_q-, where q is an integer from 2 to 5, and where R⁵ represents hydrogen, hydroxy, protected hydroxy or C_1-5 alkyl.

25. A compound according to claim 9, which is 17(Z)-1α,25-dihydroxy-17(20)-dehydro-2-methylene-19-nor-vitamin D₃ having the following formula for use in treating or preventing obesity of an animal, inhibiting adipocyte differentiation, inhibiting SCD-1 gene transcription, and/or reducing body fat in an animal.

26. The compound for use according to any of claims 12 to 25, wherein the vitamin D analog is to be administered orally, parenterally or transdermally.

27. The compound for use according to claims 12, 13, 20 or 21, wherein the vitamin D analog is to be administered topically.

28. The compound for use according to any of claims 12 to 25, wherein the vitamin D analog is to be administered in a dosage of from 0.01µg/day to 1000µg/day.

29. The compound for use according to claims 24 or 25, wherein the animal is a human, a domestic animal or an agricultural animal.

Ansprüche

1. Verbindung mit der Formel:

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy oder C_1-5-Alkyl bedeutet.

2. Verbindung nach Anspruch 1, wobei Y₂ Wasserstoff ist.

3. Verbindung nach Anspruch 1, wobei Y₁ Wasserstoff ist.

4. Verbindung nach Anspruch 1, wobei Y₁, Y₂ und R₅ Wasserstoff sind.

5. Verbindung nach Anspruch 1, die ein 17,20(Z)-Dehydrovitamin D Analogon mit der Formel:

ist, wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils ausgewählt werden aus Wasserstoff oder einer Hydroxyschutzgruppe.

6. Verbindung nach Anspruch 5, wobei Y₂ Wasserstoff ist.

7. Verbindung nach Anspruch 5, wobei Y₁ Wasserstoff ist.

8. Verbindung nach Anspruch 5, wobei Y₁ und Y₂ jeweils t-Butyldimethylsilyl sind.

9. Verbindung nach Anspruch 1, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ mit der Formel;

ist.

10. Pharmazeutische Zusammensetzung enthaltend eine effektive Menge wenigstens einer Verbindung nach Anspruch 1, 5 oder 9, zusammen mit einem pharmazeutisch akzeptablen Exzipienten.

11. Pharmazeutische Zusammensetzung nach Anspruch 10, wobei die effektive Menge von 0,01 µg bis 1000 µg pro Gramm der Zusammensetzung, vorzugsweise von 0,1 µg bis 500 µg pro Gramm der Zusammensetzung, umfasst.

12. Verbindung gemäß Anspruch 1, die ein 17(20)-Dehydrovitamin D Analogon der nachfolgenden Formel ist, zur Verwendung bei der Behandlung von Psoriasis:

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy oder C_1-5-Alkyl bedeutet.

13. Verbindung nach Anspruch 9, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ der nachfolgenden Formel ist, zur Verwendung bei der Behandlung von Psoriasis.

14. Verbindung nach Anspruch 1, die ein 17(20)-Dehydrovitamin D Analogon der nachfolgenden Formel ist, zur Verwendung bei der Behandlung einer Krankheit, die ausgewählt wird aus der Gruppe, die aus Leukämie, Colonkrebs, Brustkrebs, Hautkrebs oder Prostatakrebs besteht:

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy oder C_1-5-Alkyl bedeutet.

15. Verbindung nach Anspruch 9, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ der nachfolgenden Formel ist, zur Verwendung bei der Behandlung einer Krankheit, die ausgewählt wird aus der Gruppe, die aus Leukämie, Colonkrebs, Brustkrebs, Hautkrebs oder Prostatakrebs besteht.

16. Verbindung nach Anspruch 1, die ein 17(20)-Dehydrovitamin D Analogon der nachfolgenden Formel ist, zur Verwendung bei der Behandlung einer Autoimmunkrankheit, die aus der Gruppe, die aus Multipler Sklerose, Lupus, Diabetes Mellitus, Host-versus-Graft-Abstoßung und Abstoßung von Organtransplantaten besteht, ausgewählt wird:

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy oder C_1-5-Alkyl bedeutet.

17. Verbindung nach Anspruch 9, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ der nachfolgenden Formel ist, zur Verwendung bei der Behandlung einer Autoimmunkrankheit, die aus der Gruppe, die aus Multipler Sklerose, Lupus, Diabetes Mellitus, Host-versus-Graft-Abstoßung und Abstoßung von Organtransplantaten besteht, ausgewählt wird.

18. Verbindung nach Anspruch 1, die ein 17(20)-Dehydrovitamin D Analogon der nachfolgenden Formel ist, zur Verwendung bei der Behandlung einer entzündlichen Erkrankung, die aus der Gruppe, die aus rheumatoider Arthritis, Asthma und entzündlichen Darmerkrankungen besteht, ausgewählt wird

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy oder C_1-5-Alkyl bedeutet.

19. Verbindung nach Anspruch 9, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ der nachfolgenden Formel ist, zur Verwendung bei der Behandlung einer entzündlichen Erkrankung, die aus der Gruppe, die aus rheumatoider Arthritis, Asthma, und entzündlichen Darmerkrankungen besteht, ausgewählt wird.

20. Verbindung nach Anspruch 1, die ein 17(20)-Dehydrovitamin D Analogon der nachfolgenden Formel ist, zur Verwendung bei der Behandlung eines Hautzustandes, der aus der Gruppe, die aus Falten, mangelnder ausreichender Hautfestigkeit, mangelnder ausreichender dermaler Hydration und ungenügender Sebum-Sekretion besteht, ausgewählt wird:

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluorane, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy oder C_1-5-Alkyl bedeutet.

21. Verbindung nach Anspruch 9, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ der nachfolgenden Formel ist, zur Verwendung bei der Behandlung eines Hautzustandes, der aus der Gruppe, die aus Falten, mangelnder ausreichender Hautfestigkeit, mangelnder ausreichender dermaler Hydration, und ungenügender Sebum-Sekretion besteht, ausgewählt wird.

22. Verbindung nach Anspruch 1, die ein 17(20)-Dehydrovitamin D Analogon der nachfolgenden Formel ist, zur Verwendung bei der Behandlung von renaler Osteodystrophie:

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy, oder C_1-5-Alkyl bedeutet.

23. Verbindung nach Anspruch 9, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ der nachfolgenden Formel ist, zur Verwendung bei der Behandlung von renaler Osteodystrophie.

24. Verbindung nach Anspruch 1, die ein 17(20)-Dehydrovitamin D Analogon der nachfolgenden Formel ist, zur Verwendung bei der Behandlung oder Prävention von Obesitas in einem Tier, Inhibition der Adipozytendifferentiation, Inhibition der SCD-1 Gentranskription, und/oder Reduzierung des Körperfetts in einem Tier

wobei Y₁ und Y₂, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff und einer Hydroxyschutzgruppe besteht, ausgewählt wird, wobei R₁₁ und R₁₂ jeweils Wasserstoff sind, wobei R₆ und R₇, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder R₆ und R₇, wenn zusammengefasst, die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, oder R₆ und R₇, wenn zusammengefasst, die Gruppe =CR₈R₉ bedeuten können, wobei R₈ und R₉, die gleich oder verschieden sein können, jeweils aus der Gruppe, die aus Wasserstoff, Alkyl, Hydroxyalkyl, Fluoralkyl, Hydroxy und Alkoxy besteht, ausgewählt wird, oder, wenn zusammengefasst, R₈ und R₉ die Gruppe -(CH₂)_x- bedeuten können, wobei x eine ganze Zahl von 2 bis 5 ist, und wobei die Gruppe R eine Seitenkette bedeutet, die durch die Struktur

dargestellt wird, wobei die Seitenkette und 17-en Doppelbindung in der Z-Konfiguration ist und wobei Z in der obigen Seitenkettenstruktur ausgewählt wird aus Y, -OY, -CH₂OY, -C≡CY und CH=CHY, wobei die Doppelbindung in der Seitenkette eine cis oder trans Geometrie haben kann, und wobei Y ausgewählt wird aus Wasserstoff, Methyl, -COR⁵ und einem Rest der Struktur:

wobei m und n unabhängig voneinander ganze Zahlen von 0 bis 5 bedeuten, wobei R¹ ausgewählt wird aus Wasserstoff, Deuterium, Hydroxy, geschütztes Hydroxy, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei jede von R², R³ und R⁴ unabhängig voneinander ausgewählt wird aus Deuterium, Deuteroalkyl, Wasserstoff, Fluor, Trifluormethyl und C_1-5-Alkyl, das geradkettig oder verzweigt sein kann und optional einen Hydroxy- oder geschützten Hydroxy-Substituenten trägt, und wobei R¹ und R², wenn zusammengefasst, eine Oxogruppe oder eine Alkylidengruppe der allgemeinen Formel C_kH_2k-, wobei k ganzzahlig ist, die Gruppe =CR²R³ oder die Gruppe -(CH₂)_p-, wobei p eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R³ und R⁴, wenn zusammengefasst, eine Oxogruppe oder die Gruppe -(CH₂)_q-, wobei q eine ganze Zahl von 2 bis 5 ist, bedeuten, und wobei R⁵ Wasserstoff, Hydroxy, geschütztes Hydroxy oder C_1-5-Alkyl bedeutet.

25. Verbindung nach Anspruch 9, die 17(Z)-1α,25-Dihydroxy-17(20)-dehydro-2-methylen-19-nor-vitamin D₃ der nachfolgenden Formel ist, zur Verwendung bei der Behandlung oder Prävention von Obesitas in einem Tier, Inhibition der Adipozytendifferentiation, Inhibition der SCD-1 Gentranskription, und/oder Reduzierung des Körperfetts in einem Tier.

26. Verbindung zur Verwendung nach einem der Ansprüche 12 bis 25, wobei das Vitamin D Analogon oral, parenteral oder transdermal verabreicht wird.

27. Verbindung zur Verwendung nach einem der Ansprüche 12, 13, 20 oder 21, wobei das Vitamin D Analogon topisch verabreicht wird.

28. Verbindung zur Verwendung nach einem der Ansprüche 12 bis 25, wobei das Vitamin D Analogon in einer Dosis von 0,01 µg/Tag bis 1000 µg/Tag verabreicht wird.

29. Verbindung zur Verwendung nach Anspruch 24 oder 25, wobei das Tier ein Mensch, Haustier oder landwirtschaftlich genutztes Tier ist.

Revendications

1. Composé de formule

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est un nombre entier valant de 2 à 5,
et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment, un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

2. Composé conforme à la revendication 1, dans lequel Y₂ représente un atome d'hydrogène.

3. Composé conforme à la revendication 1, dans lequel Y₁ représente un atome d'hydrogène.

4. Composé conforme à la revendication 1, dans lequel Y₁, Y₂ et R⁵ représentent des atomes d'hydrogène.

5. Composé conforme à la revendication 1, qui est un analogue de 17,20(Z)-déhydro-vitamine D, de formule

dans laquelle Y₁ et Y₂ représentent des entités qui peuvent être identiques
ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur.

6. Composé conforme à la revendication 5, dans lequel Y₂ représente un atome d'hydrogène.

7. Composé conforme à la revendication 5, dans lequel Y₁ représente un atome d'hydrogène.

8. Composé conforme à la revendication 5, dans lequel Y₁ et Y₂ représentent tous les deux des groupes tertiobutyl-diméthyl-silyle.

9. Composé conforme à la revendication 1, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule

10. Composition pharmaceutique contenant au moins un composé conforme à la revendication 1, 5 ou 9, en une quantité efficace, ainsi qu'un excipient pharmacologiquement admissible.

11. Composition pharmaceutique conforme à la revendication 10, dans laquelle ladite quantité efficace vaut de 0,01 µg à 1000 µg par gramme de composition, et de préférence de 0,1 µg à 500 µg par gramme de composition.

12. Composé conforme à la revendication 1, qui est un analogue de 17(20)-déhydro-vitamine D de formule suivante, pour utilisation dans le traitement du psoriasis :

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est
un nombre entier valant de 2 à 5, et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment, un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

13. Composé conforme à la revendication 9, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule suivante, pour utilisation dans le traitement du psoriasis :

14. Composé conforme à la revendication 1, qui est un analogue de 17(20)-déhydro-vitamine D de formule suivante, pour utilisation dans le traitement d'une maladie choisie dans l'ensemble constitué par une leucémie, un cancer du côlon, un cancer du sein, un cancer de la peau et un cancer de la prostate :

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est
un nombre entier valant de 2 à 5, et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis
ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment, un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

15. Composé conforme à la revendication 9, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule suivante, pour utilisation dans le traitement d'une maladie choisie dans l'ensemble constitué par une leucémie, un cancer du côlon, un cancer du sein, un cancer de la peau et un cancer de la prostate :

16. Composé conforme à la revendication 1, qui est un analogue de 17(20)-déhydro-vitamine D de formule suivante, pour utilisation dans le traitement d'une maladie auto-immune choisie dans l'ensemble formé par une sclérose en plaques, un lupus, un diabète, une réaction de rejet hôte contre greffon, et un rejet d'organe transplanté :

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est
un nombre entier valant de 2 à 5, et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment, un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

17. Composé conforme à la revendication 9, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule suivante, pour utilisation dans le traitement d'une maladie auto-immune choisie dans l'ensemble formé par une sclérose en plaques, un lupus, un diabète, une réaction de rejet hôte contre greffon, et un rejet d'organe transplanté :

18. Composé conforme à la revendication 1, qui est un analogue de 17(20)-déhydro-vitamine D de formule suivante, pour utilisation dans le traitement d'une maladie inflammatoire choisie dans l'ensemble constitué par la polyarthrite rhumatoïde, l'asthme et les maladies intestinales inflammatoires :

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est
un nombre entier valant de 2 à 5, et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis
ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment, un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène
ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

19. Composé conforme à la revendication 9, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule suivante, pour utilisation dans le traitement d'une maladie inflammatoire choisie dans l'ensemble constitué par la polyarthrite rhumatoïde, l'asthme et les maladies intestinales inflammatoires :

20. Composé conforme à la revendication 1, qui est un analogue de 17(20)-déhydro-vitamine D de formule suivante, pour utilisation dans le traitement d'un état de la peau choisi dans l'ensemble constitué par des rides, un manque de fermeté adéquate de la peau, un manque d'hydratation adéquate du derme et une sécrétion insuffisante de sébum :

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est
un nombre entier valant de 2 à 5, et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment, un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène
ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

21. Composé conforme à la revendication 9, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule suivante, pour utilisation dans le traitement d'un état de la peau choisi dans l'ensemble constitué par des rides, un manque de fermeté adéquate de la peau, un manque d'hydratation adéquate du derme et une sécrétion insuffisante de sébum :

22. Composé conforme à la revendication 1, qui est un analogue de 17(20)-déhydro-vitamine D de formule suivante, pour utilisation dans le traitement de l'ostéodystrophie rénale :

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est un nombre entier valant de 2 à 5, et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment,
un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

23. Composé conforme à la revendication 9, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule suivante, pour utilisation dans le traitement de l'ostéodystrophie rénale :

24. Composé conforme à la revendication 1, qui est un analogue de 17(20)-déhydro-vitamine D de formule suivante, pour utilisation dans le traitement ou la prévention de l'obésité chez un animal, l'inhibition de la différentiation des adipocytes, l'inhibition de la transcription du gène SCD-1, et/ou la réduction des graisses corporelles chez un animal :

dans laquelle
Y₁ et Y₂ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune parmi un atome d'hydrogène et un groupe hydroxy-protecteur,
R₁₁ et R₁₂ représentent chacun un atome d'hydrogène,
R₆ et R₇ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₆ et R₇ représentent ensemble un groupe de formule -(CH₂)_x-
où x représente un nombre entier valant de 2 à 5, ou encore R₆ et R₇ représentent ensemble un groupe de formule =CR₈R₉ où R₈ et R₉ représentent des entités qui peuvent être identiques ou différentes et qui sont choisies chacune dans l'ensemble formé par un atome d'hydrogène et les groupes alkyle, hydroxyalkyle, fluoroalkyle, hydroxyle et alcoxy, ou R₈ et R₉ représentent ensemble un groupe de formule -(CH₂)_x- où l'indice x est un nombre entier valant de 2 à 5,
et R représente une chaîne latérale présentant la structure

dans laquelle la double liaison reliant la chaîne latérale à l'atome de carbone en position 17 présente la configuration Z, et le symbole Z représente une entité symbolisée par -Y, -OY, -CH₂OY, -C≡CY ou -CH=CHY où la double liaison de la chaîne latérale peut présenter la configuration cis
ou trans et Y représente une entité choisie parmi un atome d'hydrogène, un groupe méthyle, un groupe de formule -COR⁵ et un reste de formule

dans laquelle les indices m et n représentent chacun, indépendamment, un nombre entier valant de 0 à 5, R¹ représente une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes hydroxyle, hydroxyle protégé, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, les symboles R², R³ et R⁴ représentent chacun, indépendamment, une entité choisie parmi les atomes d'hydrogène, de deutérium et de fluor et les groupes deutéroalkyle, trifluorométhyle et alkyle en C_1-5, ces derniers pouvant posséder une chaîne linéaire ou ramifiée et porter en option un substituant hydroxy ou hydroxy protégé, ou bien R¹ et R² représentent ensemble un substituant oxo, un groupe alkylidène de formule générale C_kH_2k où l'indice k est un nombre entier, un groupe symbolisé par =CR²R³, ou un groupe de formule -(CH₂)_p- où l'indice p est un nombre entier valant de 2 à 5, ou encore R³ et R⁴ représentent ensemble un substituant oxo ou un groupe de formule -(CH₂)_q- où l'indice q est un nombre entier valant de 2 à 5, et où R⁵ représente un atome d'hydrogène ou un groupe hydroxyle, hydroxyle protégé, ou alkyle en C_1-5.

25. Composé conforme à la revendication 9, qui est la 17(Z)-1α,25-dihydroxy-17(20)-déhydro-2-méthylène-19-nor-vitamine D₃, de formule suivante, pour utilisation dans le traitement ou la prévention de l'obésité chez un animal, l'inhibition de la différentiation des adipocytes, l'inhibition de la transcription du gène SCD-1, et/ou la réduction des graisses corporelles chez un animal :

26. Composé pour utilisation, conforme à l'une des revendications 12 à 25, l'analogue de vitamine D devant être administré par voie orale, parentérale ou transdermique.

27. Composé pour utilisation, conforme à l'une des revendications 12, 13, 20 et 21, l'analogue de vitamine D devant être administré localement.

28. Composé pour utilisation, conforme à l'une des revendications 12 à 25, l'analogue de vitamine D devant être administré en une dose de 0,01 µg par jour à 1000 µg par jour.

29. Composé pour utilisation, conforme à la revendication 24 ou 25, l'animal étant un être humain, un animal domestique ou un animal d'élevage.

Drawing