(19)
(11) EP 1 921 147 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
21.05.2008 Bulletin 2008/21

(43) Date of publication A2:
14.05.2008 Bulletin 2008/20

(21) Application number: 07023832.4

(22) Date of filing: 23.02.1995
(51) International Patent Classification (IPC): 
C12N 15/57(2006.01)
C12N 9/54(2006.01)
C11D 3/386(2006.01)
 C12N 9/52(2006.01)
C12N 9/56(2006.01)
(84) Designated Contracting States:
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

(30) Priority: 24.02.1994 US 201120
17.01.1995 US 373818

(62) Application number of the earlier application in accordance with Art. 76 EPC:
95912559.2 / 0701605

(71) Applicant: Henkel Kommanditgesellschaft auf Aktien
40589 Düsseldorf (DE)

(72) Inventors:
  • Christianson, Teresa A.
    Petaluma, CA 94954 (US)
  • Goddette, Dean W.
    Chelmsford, MA 01824 (US)
  • Kottwitz, Beatrix
    40699 Erkrath (DE)
  • Maurer, Karl-Heinz
    40699 Erkrath (DE)
  • Paech, Christian G.
    Daly City, CA 94014 (US)
  • Pochandke, Winfried
    41468 Neuss (DE)
  • Poethkow, Jörg
    41468 Neuss (DE)
  • Schmidt, Irmgard
    42697 Solingen (DE)
  • Upadek, Horst
    45481 Mühlheim (DE)
  • Weiss, Albrecht
    40764 Langenfeld (DE)
  • Tang, Maria R.
    Fairfield, CA 94533 (US)
  • Wilson, Charles R.
    Santa Rosa, CA 95401 (US)

   


(54) Improved enzymes and detergents containing them


(57) This invention relates to proteases with improved application properties in cleaners and detergents. The improvements are achieved by substituting positively charged or uncharged amion acids in the substrate binding region of the wild type subtilisin protease. In particular, the present invention relates to a mutant subtilisin protease, characterized by at least one amino acid alteration which results in a reduced positive charge or an increased negative charge in the region of the substrate binding pocket, wherein said amino acid alteration is R99E,R99G,R99A or R99S.





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