(19)
(11) EP 1 969 127 B8

(12) CORRECTED NEW EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B2)

(48) Corrigendum issued on:
13.12.2017 Bulletin 2017/50

(45) Date of publication and mentionof the opposition decision:
12.07.2017 Bulletin 2017/28

(45) Mention of the grant of the patent:
18.06.2014 Bulletin 2014/25

(21) Application number: 06848864.2

(22) Date of filing: 21.12.2006
(51) International Patent Classification (IPC): 
C12N 15/57(2006.01)
C12P 21/02(2006.01)
(86) International application number:
PCT/US2006/048954
(87) International publication number:
WO 2007/075976 (05.07.2007 Gazette 2007/27)

(54)

METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS

VERFAHREN ZUR HERSTELLUNG BIOLOGISCH WIRKSAMER VITAMIN-K-ABHÄNGIGER PROTEINE MIT REKOMBINANTEN VERFAHREN

PROCÉDÉ DE PRODUCTION DE PROTÉINES DÉPENDANTES DE LA VITAMINE K BIOLOGIQUEMENT ACTIVES PAR DES PROCÉDÉS RECOMBINANTS


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

(30) Priority: 21.12.2005 US 752642 P

(43) Date of publication of application:
17.09.2008 Bulletin 2008/38

(60) Divisional application:
11157194.9 / 2385125

(73) Proprietors:
  • Aptevo BioTherapeutics LLC
    Seattle WA 98121 (US)
  • University of North Carolina at Chapel Hill
    Chapel Hill, NC 27599 (US)

(72) Inventors:
  • DROHAN, William, N.
    Springfield, VA 22152 (US)
  • GRIFFITH, Michael, J.
    San Juan Capistrano, California 92675 (US)
  • TAYLOR, John, R.
    New York, New York 10128 (US)
  • STAFFORD, Darrel, W.
    Carrboro, North Carolina 27599 (US)

(74) Representative: Gill Jennings & Every LLP 
The Broadgate Tower 20 Primrose Street
London EC2A 2ES
London EC2A 2ES (GB)


(56) References cited: : 
EP-A2- 2 385 125
WO-A-92/01795
WO-A-92/19636
WO-A-2005/030039
WO-A-2006/067116
WO-A-2006/101474
WO-A2-2005/030039
US-A- 5 888 809
WO-A-02/29045
WO-A-92/09698
WO-A-96/34966
WO-A-2005/038019
WO-A-2006/089613
WO-A-2006/110083
US-A- 5 888 809
US-A1- 2006 121 574
   
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  • RUNNING DEER JENNIFER ET AL: "High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1alpha gene", BIOTECHNOLOGY PROGRESS, AMERICAN INSTITUTE OF CHEMICAL ENGINEERS, US, vol. 20, no. 3, 10 March 2004 (2004-03-10) , pages 880-889, XP002557337, ISSN: 8756-7938, DOI: 10.1021/BP034383R [retrieved on 2008-09-05]
  • KAUFMAN R J ET AL: "EXPRESSION PURIFICATION AND CHARACTERIZATION OF RECOMBINANT GAMMA CARBOXYLATED FACTOR-IX SYNTHESIZED IN CHINESE HAMSTER OVARY CELLS", JOURNAL OF BIOLOGICAL CHEMISTRY.(MICROFILMS), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 261, no. 21, 25 July 1986 (1986-07-25), pages 9622-9628, XP002375183,
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  • GGCX (P38435), Molecular Target Synopsis, downloaded Feb. 25, 2015
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