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<ep-patent-document id="EP06848864B8W1" file="EP06848864W1B8.xml" lang="en" country="EP" doc-number="1969127" kind="B8" correction-code="W1" date-publ="20171213" status="c" dtd-version="ep-patent-document-v1-5">
<SDOBI lang="en"><B000><eptags><B001EP>ATBECHDEDKESFRGBGRITLILUNLSEMCPTIESILTLVFIRO..CY..TRBGCZEEHUPLSK....IS..............................</B001EP><B003EP>*</B003EP><B005EP>J</B005EP><B007EP>BDM Ver 0.1.63 (23 May 2017) -  2999001/0</B007EP></eptags></B000><B100><B110>1969127</B110><B120><B121>CORRECTED NEW EUROPEAN PATENT SPECIFICATION</B121></B120><B130>B8</B130><B132EP>B2</B132EP><B140><date>20171213</date></B140><B150><B151>W1</B151><B153>73</B153><B155><B1551>de</B1551><B1552>Bibliographie</B1552><B1551>en</B1551><B1552>Bibliography</B1552><B1551>fr</B1551><B1552>Bibliographie</B1552></B155></B150><B190>EP</B190></B100><B200><B210>06848864.2</B210><B220><date>20061221</date></B220><B240><B241><date>20080718</date></B241><B242><date>20090223</date></B242><B243><date>20170712</date></B243></B240><B250>en</B250><B251EP>en</B251EP><B260>en</B260></B200><B300><B310>752642 P</B310><B320><date>20051221</date></B320><B330><ctry>US</ctry></B330></B300><B400><B405><date>20171213</date><bnum>201750</bnum></B405><B430><date>20080917</date><bnum>200838</bnum></B430><B450><date>20140618</date><bnum>201425</bnum></B450><B452EP><date>20140108</date></B452EP><B477><date>20170712</date><bnum>201728</bnum></B477><B480><date>20171213</date><bnum>201750</bnum></B480></B400><B500><B510EP><classification-ipcr sequence="1"><text>C12N  15/57        20060101AFI20070824BHEP        </text></classification-ipcr><classification-ipcr sequence="2"><text>C12P  21/02        20060101ALI20070824BHEP        </text></classification-ipcr></B510EP><B540><B541>de</B541><B542>VERFAHREN ZUR HERSTELLUNG BIOLOGISCH WIRKSAMER VITAMIN-K-ABHÄNGIGER PROTEINE MIT REKOMBINANTEN VERFAHREN</B542><B541>en</B541><B542>METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS</B542><B541>fr</B541><B542>PROCÉDÉ DE PRODUCTION DE PROTÉINES DÉPENDANTES DE LA VITAMINE K BIOLOGIQUEMENT ACTIVES PAR DES PROCÉDÉS RECOMBINANTS</B542></B540><B560><B561><text>EP-A2- 2 385 125</text></B561><B561><text>WO-A-02/29045</text></B561><B561><text>WO-A-92/01795</text></B561><B561><text>WO-A-92/09698</text></B561><B561><text>WO-A-92/19636</text></B561><B561><text>WO-A-96/34966</text></B561><B561><text>WO-A-2005/030039</text></B561><B561><text>WO-A-2005/038019</text></B561><B561><text>WO-A-2006/067116</text></B561><B561><text>WO-A-2006/089613</text></B561><B561><text>WO-A-2006/101474</text></B561><B561><text>WO-A-2006/110083</text></B561><B561><text>WO-A2-2005/030039</text></B561><B561><text>US-A- 5 888 809</text></B561><B561><text>US-A- 5 888 809</text></B561><B561><text>US-A1- 2006 121 574</text></B561><B562><text>WAJIH N ET AL: "Increased production of functional recombinant human clotting factor IX by baby hamster kidney cells engineered to overexpress VKORC1, the vitamin K 2,3-epoxide-reducing enzyme of the vitamin K cycle" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 280, no. 36, 19 July 2005 (2005-07-19), pages 31603-31607, XP002375182 ISSN: 0021-9258</text></B562><B562><text>SUN YAN-MEI ET AL: "Vitamin K epoxide reductase significantly improves carboxylation in a cell line overexpressing factor X" BLOOD, W.B.SAUNDERS COMPANY, ORLANDO, FL, US, vol. 106, no. 12, 4 August 2005 (2005-08-04), pages 3811-3815, XP002377556 ISSN: 0006-4971</text></B562><B562><text>WASLEY L C ET AL: "PACE/FURIN CAN PROCESS THE VITAMIN K-DEPENDENT PRO-FACTOR IX PRECURSOR WITHIN THE SECRETORY PATHWAY" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 268, no. 12, 25 April 1993 (1993-04-25), pages 8458-8465, XP001094106 ISSN: 0021-9258</text></B562><B562><text>REHEMTULLA A ET AL: "In vitro and in vivo functional characterization of bovine vitamin K-dependent gamma-carboxylase expressed in Chinese hamster ovary cells" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 90, no. 10, May 1993 (1993-05), pages 4611-4615, XP002204115 ISSN: 0027-8424</text></B562><B562><text>WAJIH NADEEM ET AL: "Engineering of a recombinant vitamin K-dependent gamma-carboxylation system with enhanced gamma-carboxyglutamic acid forming capacity - Evidence for a functional CXXC redox center in the system" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,, US, vol. 280, no. 11, 7 January 2005 (2005-01-07), pages 10540-10547, XP002377554 ISSN: 0021-9258</text></B562><B562><text>ROTH DAVID A ET AL: "Human recombinant factor IX: Safety and efficacy studies in hemophilia B patients previously treated with plasma-derived factor IX concentrates" BLOOD, vol. 98, no. 13, 15 December 2001 (2001-12-15), pages 3600-3606, XP002442457 ISSN: 0006-4971</text></B562><B562><text>RUNNING DEER JENNIFER ET AL: "High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1alpha gene", BIOTECHNOLOGY PROGRESS, AMERICAN INSTITUTE OF CHEMICAL ENGINEERS, US, vol. 20, no. 3, 10 March 2004 (2004-03-10) , pages 880-889, XP002557337, ISSN: 8756-7938, DOI: 10.1021/BP034383R [retrieved on 2008-09-05]</text></B562><B562><text>KAUFMAN R J ET AL: "EXPRESSION PURIFICATION AND CHARACTERIZATION OF RECOMBINANT GAMMA CARBOXYLATED FACTOR-IX SYNTHESIZED IN CHINESE HAMSTER OVARY CELLS", JOURNAL OF BIOLOGICAL CHEMISTRY.(MICROFILMS), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 261, no. 21, 25 July 1986 (1986-07-25), pages 9622-9628, XP002375183,</text></B562><B562><text>TERUYA K. ET AL: 'An approach to further enhance the cellular productivity of exogenous protein hyper-producing Chinese hamster ovary (CHO) cells' CYTOTECHNOLOGY vol. 47, 2005, pages 29 - 36</text></B562><B562><text>WAJIH N. ET AL: 'The inhibitory effect of calumenin on the vitamin K-dependent y-carboxylation stistem' THE JOURNAL OF BIOLOGICAL CHEMISTRY vol. 279, no. 24, 2004, pages 25276 - 25283</text></B562><B562><text>WALSH G. ET AL: 'Post-translational modifications in the context of therapeutic proteins' NATURE BIOTECHNOLOGY vol. 24, no. 10, October 2006, pages 1241 - 1252</text></B562><B562><text>RUNNING DEERE J. ET AL: 'High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1a gene' BIOTECHNOL. PROG. vol. 20, 2004, pages 880 - 889</text></B562><B562><text>GGCX (P38435), Molecular Target Synopsis, downloaded Feb. 25, 2015</text></B562><B562><text>NAKAI H. ET AL: 'Adeno-associated viral vector-mediated gene transfer of human blood coagulation factor IX into mouse liver' BLOOD vol. 91, no. 12, 15 June 1998, pages 4600 - 4607</text></B562><B562><text>KAUFMAN R.J. ET AL: 'Expression, purification, and characterization of recombinant y-carboxylated factor IX synthesized in Chinese hamster ovary cells' THE JOURNAL OF BIOLOGICAL CHEMISTRY vol. 261, no. 21, 1986, pages 9622 - 9628</text></B562><B562><text>CLS Behring/Coagulation Factor IX (Human) Mononine®</text></B562><B562><text>Mononine 1000 IU (https://www.medicines.org.uk/emc/medicine/ 14372), downloaded on March 17, 2015</text></B562></B560></B500><B600><B620EP><parent><cdoc><dnum><anum>11157194.9</anum><pnum>2385125</pnum></dnum><date>20110307</date></cdoc></parent></B620EP></B600><B700><B720><B721><snm>DROHAN, William, N.</snm><adr><str>8300 Botsford Ct.</str><city>Springfield, VA 22152</city><ctry>US</ctry></adr></B721><B721><snm>GRIFFITH, Michael, J.</snm><adr><str>28761 Via Buena Vista</str><city>San Juan Capistrano, California 92675</city><ctry>US</ctry></adr></B721><B721><snm>TAYLOR, John, R.</snm><adr><str>45 E. 89th Street, Apt. 40E</str><city>New York, New York 10128</city><ctry>US</ctry></adr></B721><B721><snm>STAFFORD, Darrel, W.</snm><adr><str>300 Rainbow Drive</str><city>Carrboro, North Carolina 27599</city><ctry>US</ctry></adr></B721></B720><B730><B731><snm>Aptevo BioTherapeutics LLC</snm><iid>101694824</iid><irf>PT066EP</irf><adr><str>2401 4th Avenue, Suite 1050</str><city>Seattle WA 98121</city><ctry>US</ctry></adr></B731><B731><snm>University of North Carolina at Chapel Hill</snm><iid>101061943</iid><irf>PT066EP</irf><adr><city>Chapel Hill, NC 27599</city><ctry>US</ctry></adr></B731></B730><B740><B741><snm>Gill Jennings &amp; Every LLP</snm><iid>101574570</iid><adr><str>The Broadgate Tower 
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