(19)
(11) EP 2 017 344 A8

(12) CORRECTED EUROPEAN PATENT APPLICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 A1)

(48) Corrigendum issued on:
13.05.2009 Bulletin 2009/20

(43) Date of publication:
21.01.2009 Bulletin 2009/04

(21) Application number: 07112895.3

(22) Date of filing: 20.07.2007
(51) International Patent Classification (IPC): 
C12N 15/60(2006.01)
C12N 15/80(2006.01)
C12P 1/02(2006.01)
 C12N 9/88(2006.01)
C12N 1/15(2006.01)
C12R 1/66(2006.01)
(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
Designated Extension States:
AL BA HR MK RS

(71) Applicant: Nederlandse Organisatie voor Toegepast- Natuurwetenschappelijk Onderzoek TNO
2628 VK Delft (NL)

(72) Inventors:
  • van der Werf, Maria Johanna
    6701 BC Wageningen (NL)
  • Caspers, Martinus Petrus Maria
    2289 EG Rijswijk (NL)
  • van Luijk, Nicole
    3524 LR Utrecht (NL)
  • Punt, Peter Jan
    3994 XT Houten (NL)

(74) Representative: van Loon, C.J.J. 
Vereenigde Johan de Wittlaan 7
2517 JR Den Haag
2517 JR Den Haag (NL)

   


(54) Production of itaconic acid


(57) The invention relates to the production of itaconic acid in micro-organisms by introducting into a suitable host cell a gene coding for the enzyme cis-aconitic acid decarboxylase, preferably derived from A. terreus. Further production of itaconic acid can be boosted by introduction of a gene coding for the enzyme aconitase and/or the enzyme 2-methylcitrate dehydratase. Further part of the invention are host cells, preferably from A. niger, provided with the above mentioned genes.