FREEZE-DRIED PRODUCT FOR TRANSFERRING NUCLEIC ACID, OLIGONUCLEIC ACID OR DERIVATIVE THEREOF

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(11)

EP 2 022 853 A8

(12)	CORRECTED EUROPEAN PATENT APPLICATION
	published in accordance with Art. 153(4) EPC

(15)	Correction information:
	Corrected version no 1 (W1 A1)

(48)	Corrigendum issued on:
	17.06.2009 Bulletin 2009/25

(43)	Date of publication:
	11.02.2009 Bulletin 2009/07

(21)	Application number: 07743437.1

(22)	Date of filing: 16.05.2007

(51)

International Patent Classification (IPC):

C12N 15/09^(2006.01)
A61K 47/18^(2006.01)
A61K 47/36^(2006.01)
A61K 47/44^(2006.01)
A61K 48/00^(2006.01)

A61K 9/19^(2006.01)
A61K 47/34^(2006.01)
A61K 47/42^(2006.01)
A61K 31/7088^(2006.01)
A61P 43/00^(2006.01)

(86)	International application number:
	PCT/JP2007/060002

(87)	International publication number:
	WO 2007/132873 (22.11.2007 Gazette 2007/47)

(84)	Designated Contracting States:
	AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

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Priority:

17.05.2006 JP 2006138201

(71)	Applicant: Koyama, Yoshiyuki
	Setagaya-ku Tokyo 158-0093 (JP)

(72)	Inventors:
	KOYAMA, Yoshiyuki Setagaya-ku, Tokyo 158-0093 (JP) ITO, Tomoko Chiba 274-0818 (JP)

(74)	Representative: HOFFMANN EITLE
	Patent- und Rechtsanwälte Arabellastrasse 4 81925 München 81925 München (DE)

(54)	FREEZE-DRIED PRODUCT FOR TRANSFERRING NUCLEIC ACID, OLIGONUCLEIC ACID OR DERIVATIVE THEREOF

(57) A freeze-dried product is provided which, together with demonstrating satisfactory ability to express function when used to introduce a gene or antisense nucleic acid and the like, enables concentration to be adjusted easily, offers easy handling and has superior storage performance.
A freeze-dried product of a complex containing a nucleic acid, oligonucleic acid or derivative thereof; a cationic polymer, or cationic lipid or aggregate containing the same; and, an anionic polymer; a method for preparing the freeze-dried product, comprising a step of forming a complex by mixing a nucleic acid, oligonucleic acid or derivative thereof; a cationic polymer, or cationic lipid or aggregate containing the same; and an anionic polymer, and a step of freeze-drying the complex; a preparation, reagent or kit containing the freeze-dried product for introducing a nucleic acid, oligonucleic acid or derivative thereof; and, a method for introducing a nucleic acid, oligonucleic acid or derivative thereof into cells that uses the freeze-dried product.