(19)
(11) EP 2 045 327 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
14.03.2012 Bulletin 2012/11

(45) Mention of the grant of the patent:
11.01.2012 Bulletin 2012/02

(21) Application number: 08168818.6

(22) Date of filing: 07.03.2006
(51) International Patent Classification (IPC): 
C12N 15/82(2006.01)

(54)

Expression enhancing intron sequences

Expressionsverstärkende Intron-Sequenz

Expression à amélioration de séquences d'intron


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

(30) Priority: 08.03.2005 US 659482 P

(43) Date of publication of application:
08.04.2009 Bulletin 2009/15

(60) Divisional application:
09176168.4 / 2166099
09176170.0 / 2166100
09176171.8 / 2166101
09176172.6 / 2169058
09176173.4 / 2166102

(62) Application number of the earlier application in accordance with Art. 76 EPC:
06708664.5 / 1859037

(73) Proprietor: BASF Plant Science GmbH
67056 Ludwigshafen (DE)

(72) Inventors:
  • Song, Hee-Sook
    Raleigh, NC 27606 (US)
  • Dammann, Christian
    Durham, NC 27705 (US)
  • Morra, Marc
    Bronx, NY 10461 (US)
  • Brown, Jeffrey A.
    Apex, NC 27502 (US)
  • Xing, Liqun
    Chapel Hill, NC 27516 (US)
  • Jia, Hongmei
    Apex, NC 27523 (US)

(74) Representative: Popp, Andreas 
BASF SE Global Intellectual Property GVX/B-C006
67056 Ludwigshafen
67056 Ludwigshafen (DE)


(56) References cited: : 
EP-A- 1 134 285
   
  • RETHMEIER N ET AL: "Intron-mediated enhancement of transgene expression in maize is a nuclear, gene-dependent process." THE PLANT JOURNAL : FOR CELL AND MOLECULAR BIOLOGY. OCT 1997, vol. 12, no. 4, October 1997 (1997-10), pages 895-899, XP002387946 ISSN: 0960-7412
  • ROSE A B ET AL: "Intron-mediated enhancement of gene expression independent of unique intron sequences and splicing." PLANT PHYSIOLOGY. FEB 2000, vol. 122, no. 2, February 2000 (2000-02), pages 535-542, XP002387947 ISSN: 0032-0889
  • DATABASE EMBL EBI, Hinxton, UK; 2 October 2004 (2004-10-02), MIYASHITA ET AL.: "Oryza rufipogon met6 gene for methallothionine, complete cds., strain:w1956" XP002570671 Database accession no. AB175917 -& GENES & GENETIC SYSTEMS JUN 2005, vol. 80, no. 3, June 2005 (2005-06), pages 173-183, XP002570672 ISSN: 1341-7568
  • ROSE ALAN B: "Requirements for intron-mediated enhancement of gene expression in Arabidopsis." RNA (NEW YORK, N.Y.) NOV 2002, vol. 8, no. 11, November 2002 (2002-11), pages 1444-1453, XP002329166 ISSN: 1355-8382
  • LUEHRSEN K R ET AL: "Intron enhancement of gene expression and the splicing efficiency of introns in maize cells." MOLECULAR & GENERAL GENETICS : MGG. JAN 1991, vol. 225, no. 1, January 1991 (1991-01), pages 81-93, XP001083782 ISSN: 0026-8925 -& DENNIS E S ET AL: "Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize." NUCLEIC ACIDS RESEARCH. 11 MAY 1984, vol. 12, no. 9, 11 May 1984 (1984-05-11), pages 3983-4000, XP002387949 ISSN: 0305-1048
  • MASCARENHAS D ET AL: "INTRON-MEDIATED ENHANCEMENT OF HETEROLOGOUS GENE EXPRESSION IN MAIZE" PLANT MOLECULAR BIOLOGY, SPRINGER, DORDRECHT, NL, vol. 15, no. 6, 1990, pages 913-920, XP002933738 ISSN: 0167-4412
  • ZANOR M I ET AL: "Isolation and expression of a barley beta-1,3-glucanase isoenzyme II gene." DNA SEQUENCE : THE JOURNAL OF DNA SEQUENCING AND MAPPING. 2000, vol. 10, no. 6, 2000, pages 395-398, XP008065988 ISSN: 1042-5179
  • BRENDEL V ET AL: "Prediction of locally optimal splice sites in plant pre-mRNA with applications to gene identification in Arabidopsis thaliana genomic DNA." NUCLEIC ACIDS RESEARCH. 15 OCT 1998, vol. 26, no. 20, 15 October 1998 (1998-10-15), pages 4748-4757, XP002387950 ISSN: 0305-1048
  • PAVY NATHALIE ET AL: "Evaluation of gene prediction software using a genomic data set: Application to Arabidopsis thaliana sequences" BIOINFORMATICS (OXFORD), vol. 15, no. 11, November 1999 (1999-11), pages 887-899, XP002387951 ISSN: 1367-4803
  • PERTEA M ET AL: "GeneSplicer: a new computational method for splice site prediction." NUCLEIC ACIDS RESEARCH. 1 MAR 2001, vol. 29, no. 5, 1 March 2001 (2001-03-01), pages 1185-1190, XP002387952 ISSN: 1362-4962
  • BRENDEL VOLKER ET AL: "Gene structure prediction from consensus spliced alignment of multiple ESTs matching the same genomic locus." BIOINFORMATICS (OXFORD, ENGLAND) 1 MAY 2004, vol. 20, no. 7, 1 May 2004 (2004-05-01), pages 1157-1169, XP002387953 ISSN: 1367-4803
   
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