(19)
(11) EP 2 048 233 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
02.05.2012 Bulletin 2012/18

(45) Mention of the grant of the patent:
16.11.2011 Bulletin 2011/46

(21) Application number: 07768165.8

(22) Date of filing: 28.06.2007
(51) International Patent Classification (IPC): 
C12N 15/09(2006.01)
C12P 21/02(2006.01)
 C12N 9/00(2006.01)
(86) International application number:
PCT/JP2007/063414
(87) International publication number:
WO 2008/001947 (03.01.2008 Gazette 2008/01)

(54)

MUTANT SepRS, AND METHOD FOR SITE-SPECIFIC INTRODUCTION OF PHOSPHOSERINE INTO PROTEIN BY USING THE SAME

SEPRS-MUTANTE UND VERFAHREN ZUR STELLENGERICHTETEN EINFÜHRUNG VON PHOSPHOSERIN IN PROTEIN UNTER VERWENDUNG DAVON

SepRS MUTANTE, ET PROCÉDÉ D'INTRODUCTION SITO-SPÉCIFIQUE D'UNE PHOSPHOSÉRINE DANS UNE PROTÉINE EN UTILISANT CETTE SepRS

(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

(30) Priority: 28.06.2006 JP 2006178642

(43) Date of publication of application:
15.04.2009 Bulletin 2009/16

(73) Proprietor: Riken
Wako-shi, Saitama 351-0198 (JP)

(72) Inventors:
  • YOKOYAMA, Shigeyuki
    Yokohama-shi, Kanagawa 2300045 (JP)
  • FUKUNAGA, Ryuya
    Tokyo 1138654 (JP)
  • SEKINE, Shun-ichi
    Tokyo 1138654 (JP)

(74) Representative: Arends, William Gerrit 
Marks & Clerk LLP 90 Long Acre
London WC2E 9RA
London WC2E 9RA (GB)


(56) References cited: : 
WO-A1-03/014354
WO-A1-2004/070024
JP-A- 2004 537 984
 WO-A1-2004/039989
WO-A2-2006/107813
   
  • FUKUNAGA R ET AL: "Phosphoserine aminoacylation of tRNA bearing an unnatural base anticodon" BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US LNKD- DOI:10.1016/J.BBRC.2008.05.078, vol. 372, no. 3, 1 August 2008 (2008-08-01), pages 480-485, XP022709964 ISSN: 0006-291X [retrieved on 2008-05-27]
  • PERICH J W ET AL: "EFFICIENT SOLUTION-PHASE SYNTHESIS OF MULTIPLE O-PHOSPHOSERYL- CONTAINING PEPTIDES RELATED TO CASEIN AND STATHERIN" INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, MUNKSGAARD, COPENHAGEN, DK, vol. 40, no. 2, 1 August 1992 (1992-08-01) , pages 81-88, XP000297256 ISSN: 0367-8377
  • WANG LEI ET AL: "EXPANDING THE GENETIC CODE" ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, ANNUAL REVIEWS INC., PALO ALTO, CA, US LNKD- DOI:10.1146/ANNUREV.BIOPHYS.35.101105.1215 07, vol. 35, 1 January 2006 (2006-01-01), pages 225-249, XP009084565 ISSN: 1056-8700
  • FUKUNAGA R. ET AL.: 'Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea' NAT. STRUCT. MOL. BIOL. vol. 14, no. 4, 11 March 2007, pages 272 - 279, XP003020797
  • DATABASE GENBANK [Online] XP003018750 Database accession no. (NP_068951)
  • KIGA D. ET AL.: 'Shifted positioning of the anticodon nucleotide residues of amber suppressor tRNA species by Escherichia coli arginyl-tRNA synthetase' EUR. J. BIOCHEM. vol. 268, no. 23, 2001, pages 6207 - 6213, XP003018751
  • SAKAMOTO K. ET AL.: 'Ikita Saibo de Chotanpakushitsu o Tsukuru - Hitennen Amino Acid o Tanpakushitsu ni Donyu suru Hoho' CHEMISTRY vol. 60, no. 4, 2005, pages 70 - 71, XP003018752
  • SAUERWALD A. ET AL.: 'RNA-dependent cysteine biosynthesis in archaea' SCIENCE vol. 307, no. 5717, 2005, pages 1969 - 1972, XP003018753
  • FUKUNAGA J. ET AL.: 'A base pair at the bottom of the anticodon stem is reciprocally preferred for discrimination of cognate tRNAs by Escherichia coli lysyl- and glutaminyl-tRNA synthetases' NUCLEIC ACIDS RES. vol. 34, no. 10, 13 June 2006, pages 3181 - 3188, XP003018754
   
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