| (19) |
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(11) |
EP 2 071 023 A8 |
| (12) |
CORRECTED EUROPEAN PATENT APPLICATION |
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Note: Bibliography reflects the latest situation |
| (15) |
Correction information: |
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Corrected version no 1 (W1 A2) |
| (48) |
Corrigendum issued on: |
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02.06.2010 Bulletin 2010/22 |
| (88) |
Date of publication A3: |
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14.10.2009 Bulletin 2009/42 |
| (43) |
Date of publication: |
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17.06.2009 Bulletin 2009/25 |
| (22) |
Date of filing: 26.10.1999 |
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| (51) |
International Patent Classification (IPC):
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| (84) |
Designated Contracting States: |
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AT BE CH CY DE DK ES FI FR GR IE IT LI LU MC NL PT SE |
| (30) |
Priority: |
28.10.1998 GB 9823468
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| (62) |
Application number of the earlier application in accordance with Art. 76 EPC: |
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99950990.4 / 1124944 |
| (71) |
Applicant: Promega Corporation |
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Madison, WI 53711-5399 (US) |
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| (72) |
Inventors: |
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- Squirrell, David James
Salisbury, Wiltshire SP43 0JQ (GB)
- Murphy, Melenie Jane
Salisbury, Wiltshire SP4 0JQ (GB)
- Leslie, Rachel Louise
Wiltshire SP4 0JQ (GB)
- Lowe, Christopher Robin
Cambridge CB2 1QT (GB)
- White, Peter John
Salisbury, SP4 0JQ
Wiltshire (GB)
- Tisi, Laurence Carlo
Cambridge CB2 4AT (GB)
- Murray, James Augustus Henry
Cambridge CB2 4AT (GB)
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| (74) |
Representative: Greaves, Carol Pauline et al |
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Greaves Brewster LLP
Indigo House
Cheddar Business Park
Wedmore Road
Cheddar, Somerset BS27 3EB
Cheddar, Somerset BS27 3EB (GB) |
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Remarks: |
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Claims 16 - 30 are deemed to be abandoned due to non-payment of the claims fees (Rule
45(3) EPC). |
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Remarks: |
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This application was filed on 23-07-2008 as a divisional application to the application
mentioned under INID code 62. |
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(57) A protein having luciferase activity and at least 60% similarity to luciferase from
Photinus pyralis, Luciola mingrelica, Luciola cruciata or
Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca,
Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of
(a) the amino acid residue corresponding to residue 214 in
Photinus pyralis luciferase;
(b) the amino acid residue corresponding to residue 232 in
Photinus pyralis luciferase;
(c) the amino acid residue corresponding to residue 295 in
Photinus pyralis luciferase;
(d) the amino acid residue corresponding to amino acid 14 of the
Photinus pyralis luciferase;
(e) the amino acid residue corresponding to amino acid 35 of the
Photinus pyralis luciferase;
(f) the amino acid residue corresponding to amino acid residue 105 of the
Photinus pyralis luciferase;
(g) the amino acid residue corresponding to amino acid residue 234 of the
Photinus pyralis luciferase;
(h) the amino acid residue corresponding to amino acid residue 420 of the
Photinus pyralis luciferase;
(i) the amino acid residue corresponding to amino acid residue 310 of the
Photinus pyralis luciferase;
is different to the amino acid which appears in the corresponding wild type sequence
and wherein the luciferase enzyme has increased thermostability as compared to an
enzyme having the amino acid of the corresponding wild-type luciferase at this position.