(19)
(11) EP 2 141 236 A8

(12) CORRECTED EUROPEAN PATENT APPLICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 A1)

(48) Corrigendum issued on:
10.03.2010 Bulletin 2010/10

(43) Date of publication:
06.01.2010 Bulletin 2010/01

(21) Application number: 08012016.5

(22) Date of filing: 03.07.2008
(51) International Patent Classification (IPC): 
C12N 15/52(2006.01)
C12P 17/02(2006.01)
 A61K 31/365(2006.01)
C07D 305/12(2006.01)
(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
Designated Extension States:
AL BA MK RS

(71) Applicant: KRKA, D.D., Novo Mesto
8501 Novo mesto (SI)

(72) Inventors:
  • Fujs, Stefan
    9201 Puconci (SI)
  • Kuscer, Enej
    1000 Ljubljana (SI)
  • Petkovic, Hrvoje
    1000 Ljubljana (SI)
  • Sladic, Gordan
    8000 Novo mesto (SI)
  • Gasparic, Ales
    8000 Novo mesto (SI)

(74) Representative: HOFFMANN EITLE 
Patent- und Rechtsanwälte Arabellastrasse 4
81925 München
81925 München (DE)

   


(54) Process for production of lipstatin and microorganisms therefore


(57) This invention relates to improved processes for the production of lipstatin and /or orlistat and means therefore. In particular, the invention relates to microorganisms characterized by abolished or reduced activity of the BKD complex, e.g. mutated branched-chain 2-oxo acid dehydrogenase (BKD) having reduced or abolished activity, nucleotide sequences suitable for achieving reduced or abolished BKD activity, and microorganisms having reduced or abolished BKD activity. The invention in particular relates to lipstatin-producing microorganisms of the genus Streptomyces. Furthermore, this invention relates to the use of these nucleotide sequences, microorganisms or BKD having reduced or abolished activity in the production of lipstatin and/or orlistat.