Technical Field
[0001] The present invention is concerned with improvements relating to detergent analysis
and in particular to improvements relating to the preparation of so-called "swatches"
for use in such analysis.
Background of the Invention:
[0002] In the art of laundry detergent formulation a "swatch" is a piece of generally flexible
material such as a fabric that has a stain applied thereto. The material can be, for
example, a fabrics made of cotton, polyester or various mixtures of natural and synthetic
fibres. The swatch can be non-woven (e.g. filter paper or nitrocellulose) or even
a piece of a hard material, for example, ceramic. The stain may include blood, milk,
ink, grass, tea, wine, spinach, gravy, chocolate, egg, cheese, clay, pigment, oil,
or mixtures of these components.
[0003] Swatches are commonly used in the evaluation of laundry detergent compositions. In
the traditional "model wash" analysis, as they are a small part of the wash-load,
the bulk of the load being made up of "ballast", which comprises various sheets of
cloth. One or more swatches (also known as "monitors") and a quantity of "ballast",
are washed under known conditions so that the degree of stain removal can be determined.
[0004] United States Patent
7122334 discloses a more modern technique for analysis of detergent compositions. In this,
a "smaller swatch" - a piece of the swatch that has been cut or otherwise removed
from the swatch of material either before or after fixing the stain to the swatch
is placed into the well of a 24, 48 or 96 well micro-titer plate. The "smaller swatch"
can also be made by applying a stain to a small piece of material. Typically these
"smaller swatches" are around 5mm in diameter. In the context of the present invention
"swatch" includes both larger and smaller swatches.
[0005] Swatches having stains of known "strength" on various types of material are commercially
available (from EMPA, St. Gallen, Switzerland; WFK-Testgewebe GmbH, Krefeld Germany;
or Center for Test Materials, Vlaardingen, The Netherlands) and/or can be made by
the practitioner (as described for example in
Morris and Prato, Textile Research Journal 52(4):280 286 (1982)).
[0006] It has been suggested, in
US 7122334 to use tea (
Camellia sinensis) and spinach (
Spinacia oleracea) as a source for stains.
[0007] Mechanically-produced plant stains are the more common source for such stains is
generally referred to as "grass". "Grass" is a very general term and in common parlance
is often used to refer to monocotyledonous green plants of the family Poaceae (also
known as the
Gramineae). As will be apparent, spinach and tea stains on clothing and other fabric articles
are less likely to occur through outdoor activity than "grass stains", and more likely
to result from spillage of food or drink whereas "grass stains" are more of a problem
on sports/outdoor clothing and/or children's clothing.
[0008] One of the main problems with the laboratory use of grass stains is that the results
show poor reproducibility. It is therefore difficult to optimise laundry detergent
compositions for the effective and efficient removal of grass stains. Much effort
has been given to exactly how the grass stain should be formed on the swatch and various
complicated devices have been proposed to solve this problem.
Brief Description of the Invention
[0009] We have determined that improved "grass stain" swatches can be prepared if the majority
of the green plant material present is mostly or all from a single species of green
plant.
[0010] Accordingly the present invention provides a swatch of fabric having an area of from
0.25 m
2 to 4x10
-6m
2, said swatch being stained in one or more patches over at least 0.1% of its surface
with debris comprising chloroplast material wherein at least 80%wt of the chloroplast
material present in a patch is from a single species of green plant other than tea
or spinach.
[0011] Preferably the fabric is cellulosic, polyester or a mixture thereof.
[0012] Preferably, the remaining 20%wt of the debris is from no more than five, preferably
three, preferably one other species. In a preferred embodiment the stain is derived
from a single species.
[0013] By ensuring that a small and known number of species of green plant are used reproducible
"grass stains" can be obtained that have a consistent level of difficulty as regards
their removal.
[0014] Preferably the debris is one or more species of the genera:
Agropyron, Agrostis, Axonopus, Bothriochloa, Bouteloua,
Fescue, Chamaemelum, Dactyloctenium, Chrysopogon,
Deschampsia, Desmodium, Dichanthium, Cynodon, Lolium,
Trifolium, Belliss, Taraxacum, Hilaria, Hydrocotyle,
Pennisetum, Leptinella, Koeleria, Ischaemum, Eremochloa,
Eragrostis, Elymus, Microlaena, Plantago, Paspalum,
Eurhynchium, Dichondra, Digitaria, Brachythecium, Polytrias,
Pratia, Puccinellia, Ranunculus, Senecio, Distichlis,
Holcus, Stenotaphrum, Urochloa, Zoysia, Trisetum and/or Poa.
[0015] Preferably the debris is from a Dicotyledone.
[0016] Preferably the debris is from one or more of the species:
Agrostis canina, Agrostis capillaris, Agrostis clavata,
Agrostis curtisii, Agrostis gigantea, Agrostis castellana,
Agrostis mertensii, Agrostis scabra, Agrostis stolonifera,
Agrostis tenuis, Agrostis palustris, Agrostis vineale,
Festuca rubra, Lolium perenne, Trifolium repens, Bellis
perennis, Taraxacum officinale, Eurhynchium praelongum,
Brachythecium rutabulum, Ranunculus repens, Holcus lanatus,
Senecio jacobaea and Plantago major, Poa trivialis, Poa.
pratensis and Poa. Annua.
[0017] Preferably, the debris is from one or more of the order
Ranunculales or the family
Asteraceae.
[0018] Many of these plants are more familiar under their common names:
Agrostis are the bent grasses (
Agrostis stolonifera - "
creeping bent" is commonly used on golf-course greens), and are widely used on lawns. The
Fescue genus are tufted grasses (used on bowling greens and for fodder),
Lolium are the ryegrasses,
Poa includes European meadowgrass, bluegrass and tussock, while
Holcus are also grasses. However many of the preferred plants are not grasses. For example,
Trifolium includes the clovers,
Bellis includes the daisy,
Taraxacum includes the dandelion,
Ranunculus includes the buttercup.
Eurhynchium is a moss genus as are the
Brachytheciaceae.
[0019] Particularly preferred are stains which comprise one or more of plants selected from
the genus
Lolium, preferably
Lolium perenne (a perennial ryegrass).
Festuca, preferably Festuca
pratensi (a meadow fescue).
Trifolium, preferably
Trifolium pratense (a red clover). Other suitable stains may comprise
Agrostis (bent grass),
trifolium repens (white clover) and/or
Taraxacum officinale (dandelion).
[0020] A swatch may comprise two or more stains which are different cultivars or varieties
of the same species. Thus, where the swatch comprises a stain derived from
trifolium repens (white clover) separate stains derived from the
Aber Dai cultivar and the
Aber Ace cultivar may be present.
[0021] It is preferred that at least one non-grass species is present and particularly preferred
that this is selected from the non-vascular plants (Bryophytes), from non-angiosperm
vascular plants or from the angiosperm families
Asteraceae, Rubiaceae or
Fabaceae. Swatches can be prepared wherein the debris derived from true grasses (Poaceae)
are less than 50%wt, preferably less than 25%wt of the material present. Swatches
may be prepared in which none of the material present is derived from grasses.
[0022] More than one species-specific stain can be present on each swatch. Preferably there
are 1-30 stained patches on a larger swatch, each covering at least 0.1% of the area
of the swatch. 4-10 patches are particularly preferred.
[0023] A smaller swatch may be uniformly stained or cut from a larger swatch.
[0024] A second aspect of the present invention subsists in a method for preparing a swatch
which comprises the step of contacting a fabric substrate in sheet form having an
area greater than 4x10
-6m
2 (greater than 4mm
2) with chloroplast-containing green plant material wherein at least 80% of the debris
is from a single species of green plant other than tea or spinach.
[0025] In this second aspect of the invention the preferred genera and species are as noted
above and the process may be repeated to give a swatch bearing a plurality of stains.
[0026] A third aspect of the present invention provides a method for determining the effectiveness
of laundry compositions which includes that step of treating swatches according to
the present invention with the laundry composition. In a particularly preferred embodiment
of the invention the laundry composition comprises at least one enzyme.
[0027] As chloroplasts contain DNA, it is relatively easy to determine the number and type
of genera and species represented on a particular swatch.
Detailed Description of the Invention
Enzymes
[0028] As noted above, in a particularly preferred embodiment of the invention the laundry
composition being tested comprises at least one enzyme. Especially contemplated enzymes
include proteases, alpha-amylases, cellulases, lipases, peroxidases/oxidases, pectate
lyases, and mannanases, or mixtures thereof.
[0029] Suitable proteases include those of animal, vegetable or microbial origin. Microbial
origin is preferred. Chemically modified or protein engineered mutants are included.
The protease may be a serine protease or a metallo protease, preferably an alkaline
microbial protease or a trypsin-like protease. Examples of alkaline proteases are
subtilisins, especially those derived from
Bacillus, e.g., subtilisin Novo, subtilisin Carlsberg, subtilisin 309, subtilisin 147 and subtilisin
168 (described in
WO 89/06279). Examples of trypsin-like proteases are trypsin (e.g. of porcine or bovine origin)
and the
Fusarium protease described in
WO 89/06270 and
WO 94/25583.
[0030] Examples of useful proteases are the variants described in
WO 92/19729,
WO 98/20115,
WO 98/20116, and
WO 98/34946, especially the variants with substitutions in one or more of the following positions:
27, 36, 57, 76, 87, 97, 101, 104, 120, 123, 167, 170, 194, 206, 218, 222, 224, 235
and 274. Preferred commercially available protease enzymes include Alcalase™, Savinase™,
Primase™, Duralase™, Dyrazym™, Esperase™, Everlase™, Polarzyme™, and Kannase™, (Novozymes
A/S), Maxatase™, Maxacal™, Maxapem™, Properase™, Purafect™, Purafect OxP™, FN2™, and
FN3™ (Genencor International Inc.).
[0031] Suitable lipases include those of bacterial or fungal origin. Chemically modified
or protein engineered mutants are included. Examples of useful lipases include lipases
from
Humicola (synonym
Thermomyces), e.g. from
H. lanuginosa (
T. lanuginosus) as described in
EP 258 068 and
EP 305 216 or from
H. insolens as described in
WO 96/13580, a
Pseudomonas lipase, e.g. from
P. alcaligenes or
P. pseudoalcaligenes (
EP 218 272),
P. cepacia (
EP 331 376),
P. stutzeri (
GB 1,372,034),
P. fluorescens, Pseudomonas sp. strain SD 705 (
WO 95/06720 and
WO 96/27002),
P. wisconsinensis (
WO 96/12012), a
Bacillus lipase, e.g. from
B. subtilis (
Dartois et al. (1993), Biochemica et Biophysica Acta, 1131, 253-360),
B. stearothermophilus (
JP 64/744992) or
B. pumilus (
WO 91/16422).
[0032] Other examples are lipase variants such as those described in
WO 92/05249,
WO 94/01541,
EP 407 225,
EP 260 105,
WO 95/35381,
WO 96/00292,
WO 95/30744,
WO 94/25578,
WO 95/14783,
WO 95/22615,
WO 97/04079 and
WO 97/07202.
[0033] Preferred commercially available lipase enzymes include Lipolase™ and Lipolase Ultra™,
Lipex™ (Novozymes A/S).
[0034] The method of the invention may be carried out in the presence of cutinase. classified
in EC 3.1.1.74. The cutinase used according to the invention may be of any origin.
Preferably cutinases are of microbial origin, in particular of bacterial, of fungal
or of yeast origin.
[0035] Cutinases are enzymes which are able to degrade cutin. In a preferred embodiment,
the cutinase is derived from a strain of
Aspergillus, in particular
Aspergillus oryzae, a strain of
Alternaria, in particular
Alternaria brassiciola, a strain of
Fusarium, in particular
Fusarium solani, Fusarium solani pisi, Fusarium roseum culmorum, or
Fusarium roseum sambucium, a strain of
Helminthosporum, in particular
Helminthosporum sativum, a strain of
Humicola, in particular
Humicola insolens, a strain of
Pseudomonas, in particular
Pseudomonas mendocina, or
Pseudomonas putida, a strain of
Rhizoctonia, in particular
Rhizoctonia solani, a strain of
Streptomyces, in particular
Streptomyces scabies, or a strain of
Ulocladium, in particular
Ulocladium consortiale.
[0036] In a most preferred embodiment the cutinase is derived from a strain of
Humicola insolens, in particular the strain
Humicola insolens DSM 1800.
Humicola insolens cutinase is described in
WO 96/13580 which is herby incorporated by reference. The cutinase may be a variant, such as
one of the variants disclosed in
WO 00/34450 and
WO 01/92502, which are hereby incorporated by reference. Preferred cutinase variants include
variants listed in Example 2 of
WO 01/92502, which is hereby specifically incorporated by reference.
[0037] Preferred commercial cutinases include NOVOZYM™ 51032 (available from Novozymes A/S,
Denmark).
[0038] The method of the invention may be carried out in the presence of phospholipase classified
as EC 3.1.1.4 and/or EC 3.1.1.32. As used herein, the term phospholipase is an enzyme
which has activity towards phospholipids. Phospholipids, such as lecithin or phosphatidylcholine,
consist of glycerol esterified with two fatty acids in an outer (sn-1) and the middle
(sn-2) positions and esterified with phosphoric acid in the third position; the phosphoric
acid, in turn, may be esterified to an amino-alcohol. Phospholipases are enzymes which
participate in the hydrolysis of phospholipids. Several types of phospholipase activity
can be distinguished, including phospholipases A
1 and A
2 which hydrolyze one fatty acyl group (in the sn-1 and sn-2 position, respectively)
to form lysophospholipid; and lysophospholipase (or phospholipase B) which can hydrolyze
the remaining fatty acyl group in lysophospholipid. Phospholipase C and phospholipase
D (phosphodiesterases) release diacyl glycerol or phosphatidic acid respectively.
[0039] The term phospholipase includes enzymes with phospholipase activity, e.g., phospholipase
A (A
1 or A
2), phospholipase B activity, phospholipase C activity or phospholipase D activity.
The term "phospholipase A" used herein in with an enzyme of the invention is intended
to cover an enzyme with Phospholipase A
1 and/or Phospholipase A
2 activity. The phospholipase activity may be provided by enzymes having other activities
as well, such as, e.g., a lipase with phospholipase activity. The phospholipase activity
may, e.g., be from a lipase with phospholipase side activity. In other embodiments
of the invention the phospholipase enzyme activity is provided by an enzyme having
essentially only phospholipase activity and wherein the phospholipase enzyme activity
is not a side activity.
[0040] The phospholipase may be of any origin, e.g., of animal origin (such as, e.g., mammalian),
e.g. from pancreas (e.g., bovine or porcine pancreas), or snake venom or bee venom.
Preferably the phospholipase may be of microbial origin, e.g., from filamentous fungi,
yeast or bacteria, such as the genus or species
Aspergillus, e.g.,
A. niger; Dictyostelium, e.g.,
D. discoideum; Mucor, e.g.
M. javanicus,
M. mucedo, M.
subtilissimus; Neurospora, e.g.
N. crassa;
Rhizomucor, e.g.,
R. pusillus; Rhizopus, e.g.
R. arrhizus, R. japonicus, R. stolonifer; Sclerotinia, e.g.,
S. libertiana;
Trichophyton, e.g.
T. rubrum; Whetzelinia, e.g.,
W. sclerotiorum; Bacillus, e.g.,
B. megaterium, B. subtilis; Citrobacter, e.g.,
C. freundii; Enterobacter, e.g.,
E. aerogenes, E. cloacae
Edwardsiella, E. tarda;
Erwinia, e.g.,
E. herbicola; Escherichia, e.g.,
E. coli; Klebsiella, e.g.,
K. pneumoniae; Proteus, e.g.,
P. vulgaris; Providencia, e.g.,
P. stuartii; Salmonella, e.g.
S. typhimurium; Serratia, e.g.,
S. liquefasciens, S. marcescens; Shigella, e.g.,
S. flexneri; Streptomyces, e.g.,
S. violeceoruber; Yersinia, e.g.,
Y. enterocolitica. Thus, the phospholipase may be fungal, e.g., from the class
Pyrenomycetes, such as the genus
Fusarium, such as a strain of
F. culmorum, F. heterosporum, F. solani, or a strain of
F. oxysporum. The phospholipase may also be from a filamentous fungus strain within the genus
Aspergillus, such as a strain of
Aspergillus awamori, Aspergillus foetidus, Aspergillus japonicus, Aspergillus niger or
Aspergillus oryzae.
[0041] Preferred phospholipases are derived from a strain of
Humicola, especially
Humicola lanuginosa. The phospholipase may be a variant, such as one of the variants disclosed in
WO 00/32758, which are hereby incorporated by reference. Preferred phospholipase variants include
variants listed in Example 5 of
WO 00/32758, which is hereby specifically incorporated by reference. In another preferred embodiment
the phospholipase is one described in
WO 04/111216, especially the variants listed in the table in Example 1.
[0042] In another preferred embodiment the phospholipase is derived from a strain of
Fusarium, especially
Fusarium oxysporum. The phospholipase may be the one concerned in
WO 98/026057 derived from Fusarium oxysporum DSM 2672, or variants thereof.
[0043] In a preferred embodiment of the invention the phospholipase is a phospholipase A
1 (EC. 3.1.1.32). In another preferred embodiment of the invention the phospholipase
is a phospholipase A
2 (EC.3.1.1.4.).
[0044] Examples of commercial phospholipases include LECITASE™ and LECITASE™ ULTRA, YIELSMAX,
or LIPOPAN F (available from Novozymes A/S, Denmark).
[0045] Suitable amylases (alpha and/or beta) include those of bacterial or fungal origin.
Chemically modified or protein engineered mutants are included. Amylases include,
for example, alpha-amylases obtained from
Bacillus, e.g. a special strain of
B. licheniformis, described in more detail in
GB 1,296,839, or the
Bacillus sp. strains disclosed in
WO 95/026397 or
WO 00/060060.
[0047] Commercially available amylases are Duramyl™, Termamyl™, Termamyl Ultra™, Natalase™,
Stainzyme™, Fungamyl™ and BAN™ (Novozymes A/S), Rapidase™ and Purastar™ (from Genencor
International Inc.).
[0048] Suitable cellulases include those of bacterial or fungal origin. Chemically modified
or protein engineered mutants are included. Suitable cellulases include cellulases
from the genera
Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, e.g. the fungal cellulases produced from
Humicola insolens, Thielavia terrestris, Myceliophthora thermophila, and
Fusarium oxysporum disclosed in
US 4,435,307,
US 5,648,263,
US 5,691,178,
US 5,776,757,
WO 89/09259,
WO 96/029397, and
WO 98/012307.
[0049] Especially suitable cellulases are the alkaline or neutral cellulases having color
care benefits. Examples of such cellulases are cellulases described in
EP 0 495 257,
EP 0 531 372,
WO 96/11262,
WO 96/29397,
WO 98/08940. Other examples are cellulase variants such as those described in
WO 94/07998,
EP 0 531 315,
US 5,457,046,
US 5,686,593,
US 5,763,254,
WO 95/24471,
WO 98/12307 and
PCT/DK98/00299.
[0050] Commercially available cellulases include Celluzyme™, Carezyme™, Endolase™, Renozyme™
(Novozymes A/S), Clazinase™ and Puradax HA™ (Genencor International Inc.), and KAC-500(B)™
(Kao Corporation).
[0051] Suitable peroxidases/oxidases include those of plant, bacterial or fungal origin.
Chemically modified or protein engineered mutants are included. Examples of useful
peroxidases include peroxidases from
Coprinus, e.g. from
C. Cinereus, and variants thereof as those described in
WO 93/24618,
WO 95/10602, and
WO 98/15257. Commercially available peroxidases include Guardzyme™ and Novozym™ 51004 (Novozymes
A/S).
[0052] Examples of pectate lyases include pectate lyases that have been cloned from different
bacterial genera such as
Erwinia, Pseudomonas, Klebsiella and
Xanthomonas, as well as from
Bacillus subtilis (
Nasser et al. (1993) FEBS Letts. 335:319-326) and
Bacillus sp. YA-14 (
Kim et al. (1994) Biosci. Biotech. Biochem. 58:947-949). Purification of pectate lyases with maximum activity in the pH range of 8-10 produced
by
Bacillus pumilus (
Dave and Vaughn (1971) J. Bacteriol. 108:166-174),
B. polymyxa (
Nagel and Vaughn (1961) Arch. Biochem. Biophys. 93:344-352),
B. stearothermophilus (
Karbassi and Vaughn (1980) Can. J. Microbiol. 26:377-384),
Bacillus sp. (
Hasegawa and Nagel (1966) J. Food Sci. 31:838-845) and
Bacillus sp. RK9 (
Kelly and Fogarty (1978) Can. J. Microbiol. 24:1164-1172) have also been described. Any of the above, as well as divalent cation-independent
and/or thermostable pectate lyases, may be used in practicing the invention. In preferred
embodiments, the pectate lyase comprises the amino acid sequence of a pectate lyase
disclosed in
Heffron et al., (1995) Mol. Plant-Microbe Interact. 8: 331-334 and
Henrissat et al., (1995) Plant Physiol. 107: 963-976. Specifically contemplated pectatel lyases are disclosed in
WO 99/27083 and
WO 99/27084. Other specifically contemplates pectate lyases derived from
Bacillus licheniformis is disclosed as in
US patent no. 6,284,524 (which document is hereby incorporated by reference). Specifically contemplated pectate
lyase variants are disclosed in
WO 02/006442, especially the variants disclosed in the Examples in
WO 02/006442 (which document is hereby incorporated by reference).
[0053] Examples of commercially available alkaline pectate lyases include BIOPREP™ and SCOURZYME™
L from Novozymes A/S, Denmark.
[0054] Examples of mannanases (EC 3.2.1.78) include mannanases of bacterial and fungal origin.
In a specific embodiment the mannanase is derived from a strain of the filamentous
fungus genus Aspergillus, preferably
Aspergillus niger or
Aspergillus aculeatus (
WO 94/25576).
WO 93/24622 discloses a mannanase isolated from
Trichoderma reseei. Mannanases have also been isolated from several bacteria, including
Bacillus organisms. For example,
Talbot et al., Appl. Environ. Microbiol., Vol.56, No. 11, pp. 3505-3510 (1990) describes a beta-mannanase derived from
Bacillus stearothermophilus. Mendoza et al., World J. Microbiol. Biotech., Vol. 10, No. 5, pp. 551-555 (1994) describes a beta-mannanase derived from
Bacillus subtilis. JP-A-03047076 discloses a beta-mannanase derived from
Bacillus sp.
JP-A-63056289 describes the production of an alkaline, thermostable beta-mannanase.
JP-A-63036775 relates to the
Bacillus microorganism FERM P-8856 which produces beta-mannanase and beta-mannosidase.
JP-A-08051975 discloses alkaline beta-mannanases from alkalophilic
Bacillus sp. AM-001. A purified mannanase from
Bacillus amyloliquefaciens is disclosed in
WO 97/11164.
WO 91/18974 describes a hemicellulase such as a glucanase, xylanase or mannanase active. Contemplated
are the alkaline family 5 and 26 mannanases derived from
Bacillus agaradhaerens, Bacillus licheniformis, Bacillus halodurans, Bacillus clausii,
Bacillus sp., and
Humicola insolens disclosed in
WO 99/64619. Especially contemplated are the
Bacillus sp. mannanases concerned in the Examples in
WO 99/64619 which document is hereby incorporated by reference.
[0055] Examples of commercially available mannanases include Mannaway™ available from Novozymes
A/S Denmark.
[0056] Any enzyme present in the composition may be stabilized using conventional stabilizing
agents, e.g., a polyol such as propylene glycol or glycerol, a sugar or sugar alcohol,
lactic acid, boric acid, or a boric acid derivative, e.g., an aromatic borate ester,
or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid, and the composition
may be formulated as described in e.g.
WO 92/19709 and
WO 92/19708.
[0057] In order that the invention may be further understood and carried forth into practice
it will be further described with reference to the following non-limiting examples.
Examples
Example 1: Preparation of Swatches
[0058] The following species were used to form "single species" swatches:
Lolium perenne - perennial ryegrass (a low phenolic grass) Festuca pratensis - meadow fescue (a high phenolic grass) Trifolium pratense (red clover)
[0059] The following species were used to form a six species "multi-species swatch" which
comprised six different stains on the same cloth.
[0060] Lolium perenne (ryegrass)
Aberglyn cultivar
Festuca rubra (red fescue)
Cezanne cultivar Agrostis (bent grass)
trifolium repens (white clover)
Aber Dai cultivar
trifolium repens (white clover)
Aber Ace cultivar
Taraxacum officinale (dandelion)
[0061] All plants were grown under standard greenhouse conditions. The physiology and biochemical
composition of cell walls/protoplasts/ etc differs as the plant progresses through
its life cycle. For consistency purposes growth under controlled conditions is required.
For stain generation, white cotton and polyester fabric was secured between the plates
of 2-4cm diameter staining mould. A small bunch of plant leaf material was shaped
into a 2-cm ball-shape and rubbed against the fabric in a circular motion for 15 seconds,
until a homogeneous stain was obtained. In the case of the multi-species swatches
this process was repeated with materials taken from a number of different plants,
so as to obtain a single swatch carrying stains from a plurality of different plants.
[0062] Stains were aged in the dark and at room temperature for 3 days prior to washing.
Example 2: Use of the swatches
[0063] Stains were washed in a Tergotometer™ under the conditions sopecified below:
Temperature |
= 37°C |
Time |
= 30min |
Agitation |
= 100rpm |
Wash liquor |
= 11 |
Water Hardness |
= 6°FH |
Product Dose (Persil Bio/ Non-) |
= 6.6g/l |
Liquid : Cloth |
= 40:1 |
Rinse |
= 2 x 2 mins in 21 demin water (FH6) |
Drying |
=o/n and at RT/ in the dark |
Spectra measurements: |
= HunterLab ™ (ΔE) |
[0064] The detergent used was Persil™ Bio and non-Bio powder. The 1*a*b and ΔE values of
grass stains were obtained before and after wash using a Hunterlab ™ calibrated against
clean cotton and polyester fabrics.
[0065] The results obtained are presented in the tables below:
Table 1
Polyester |
Cotton |
|
mean |
|
sd |
|
|
mean |
|
sd |
|
|
Non bio |
Bio |
Non bio |
Bio |
|
Non bio |
Bio |
Non bio |
Bio |
lolium |
32.7 |
37.3 |
1.6 |
1.1 |
lolium |
28.4 |
33.0 |
4.6 |
1.2 |
festuca |
24.2 |
28.7 |
1.7 |
0.9 |
festuca |
27.2 |
30.6 |
2.2 |
0.8 |
Clover |
11.2 |
30.4 |
1.5 |
1.6 |
Clover |
11.9 |
27.2 |
1.2 |
0.7 |
Table 2
Cotton |
|
mean |
|
sd |
|
|
non bio |
Bio |
non bio |
bio |
ryegrass |
28.7 |
40.3 |
2.3 |
2.3 |
fescue |
27.4 |
42.0 |
1.3 |
1.4 |
argostis |
24.4 |
40.3 |
1.5 |
1.5 |
clover trifolium |
29.8 |
36.8 |
2.1 |
2.1 |
clover trifolium II |
31.5 |
38.7 |
1.4 |
2.1 |
Dandelion |
17.7 |
33.3 |
2.7 |
1.3 |
|
Polyester |
|
mean |
|
sd |
|
|
non bio |
bio |
Non bio |
bio |
ryegrass |
33.1 |
41.6 |
2.3 |
2.3 |
fescue |
29.7 |
43.3 |
1.3 |
1.4 |
argostis |
30.8 |
36.9 |
1.5 |
1.5 |
clover trifolium |
37.3 |
38.1 |
2.1 |
2.1 |
clover trifolium II |
32.0 |
35.0 |
1.4 |
2.1 |
Dandelion |
26.5 |
32.7 |
2.7 |
1.3 |
[0066] Even from these few results it is easy to see the utility of the swatches is exploring
how a detergent composition could be improved so as to clean better. The low SD shows
good reproducibility. It is also evident that enzymes (present in the bio product)
assist in the removal of plant-derived stains.
1. A swatch of fabric having an area of from 0.25 m2 to 4x10-6m2, said swatch being stained in one or more patches over at least 0.1% of its surface
with debris comprising chloroplast material wherein at least 80%wt of the chloroplast
material present in a patch is from a single species of green plant other than tea
or spinach.
2. A swatch according to claim 1 wherein the fabric is cellulosic, polyester or a mixture
thereof.
3. A swatch according to any preceeding claim wherein the remaining 20%wt of the debris
is from no more than five, preferably three, preferably one other species.
4. A swatch according to any preceeding claim wherein at least one patch is derived from
a single species.
5. A swatch according to any preceeding claim wherein the debris are from one or more
species of the genera:
Agropyron, Agrostis, Axonopus, Bothriochloa, Bouteloua,
Fescue, Chamaemelum, Dactyloctenium, Chrysopogon,
Deschampsia, Desmodium, Dichanthium, Cynodon, Lolium,
Trifolium, Belliss, Taraxacum, Hilaria, Hydrocotyle,
Pennisetum, Leptinella, Koeleria, Ischaemum,
Eremochloa, Eragrostis, Elymus, Microlaena, Plantago,
Paspalum, Eurhynchium, Dichondra, Digitaria,
Brachythecium, Polytrias, Pratia, Puccinellia,
Ranunculus, Senecio, Distichlis, Holcus, Stenotaphrum,
Urochloa, Zoysia, Trisetum and Poa.
6. A swatch according to any preceeding claim wherein the debris are from one or more
of the species: Agrostis canina, Agrostis capillaris, Agrostis clavata, Agrostis curtisii, Agrostis gigantea, Agrostis castellana, Agrostis mertensii, Agrostis
scabra, Agrostis stolonifera, Agrostis tenuis, Agrostis palustris, Agrostis vineale,
Festuca rubra, Lolium perenne, Trifolium repens, Bellis perennis, Taraxacum officinale,
Eurhynchium praelongum, Brachythecium rutabulum, Ranunculus repens, Holcus lanatus,
Poa trivialis, Poa. pratensis and Poa. Annua.
7. A method for preparing a swatch according to any of claims 1-7 which comprises the
step of contacting a fabric substrate in sheet form having an area greater than 4x10-6m2 with chloroplast-containing green plant debris wherein at least 80% of the debris
is from a single species of green plant other than tea or spinach.
8. A method for determining the effectiveness of a laundry composition which includes
that step of treating swatches according to any of claims 1-7 with the laundry composition.
9. A method according to claim 8 wherein the laundry composition comprises at least one
enzyme.
10. A method according to claim 8 wherein the laundry composition comprises a plurality
of enzymes.