(19)
(11) EP 2 173 892 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
01.08.2012 Bulletin 2012/31

(45) Mention of the grant of the patent:
21.09.2011 Bulletin 2011/38

(21) Application number: 07859596.4

(22) Date of filing: 27.07.2007
(51) International Patent Classification (IPC): 
C12P 35/04(2006.01)
 C12P 37/04(2006.01)
(86) International application number:
PCT/IN2007/000319
(87) International publication number:
WO 2009/016642 (05.02.2009 Gazette 2009/06)

(54)

Process for the preparation of immobilised recombinant Penicillin Acylase biocatalyst from Achromobacter sp CCM 4824 expressed in E. coli BL21 CCM 7394 and its use for the synthesis of beta-lactam antiobiotics

Verfahren zur Präparation von immobilisierter rekombinanter Penicillin Acylase aus Achromobacter sp CCM 4824 exprimiert in E. coli BL21 CCM 7394 und dessen Verwendung für die Synthese von beta-Lactam Antibiotika

Procédé de préparation de l'enzyme immobilisée recominée Penicilline Acylase d'Achromobacter sp CCM 4824 exprimée dans E. coli BL21 CCM 7394 et son utilisation pour la synthèse d'antibiotiques beta-lactame

(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

(43) Date of publication of application:
14.04.2010 Bulletin 2010/15

(73) Proprietor: Fermenta Biotech Limited
MAH (IN)

(72) Inventors:
  • DATLA, Anupama
    Mumbai 400 049, Maharashtra (IN)
  • RAJASEKAR, Vyasarayani, Williams
    Thane (W) 400 610, Maharashtra (IN)
  • KYSLIK, Pavel
    Czech Republic (CZ)
  • BECKA, Stanislav
    Czech Republic (CZ)
  • KRISHNAKANT, Ashar, Trupti
    Thane (W) 400 607 Maharashtra (IN)
  • YOGESH, Zambre, Sujata
    Thane (W) 400 602 Maharashtra (IN)
  • NIKUNJ, Kumar
    Mumbai 400 019 Maharashtra (IN)

(74) Representative: Murray, Adrian D'Coligny 
W.P. Thompson & Co. 55 Drury Lane
London WC2B 5SQ
London WC2B 5SQ (GB)


(56) References cited: : 
WO-A-2004/050893
 US-B1- 6 403 356
   
  • SKROB FRANTISEK ET AL: "Novel penicillin G acylase from Achromobacter sp. CCM 4824" ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 32, no. 6, 20 May 2003 (2003-05-20), pages 738-744, XP002473789 ISSN: 0141-0229 cited in the application
  • CAO L ET AL: "CROSS-LINKED AGGREGATES OF PENICILLIN ACYLASE: ROBUST CATALYSTS FOR THE SYNTHESIS OF BETA-LACTAM ANTIBIOTICS" JOURNAL OF MOLECULAR CATALYSIS. B, ENZYMATIC, ELSEVIER, AMSTERDAM, NL, vol. 4-06, no. 11, 22 January 2001 (2001-01-22), pages 665-670, XP001079507 ISSN: 1381-1177 cited in the application
  • DE LEON A ET AL: "Periplasmic penicillin G acylase activity in recombinant Escherichia coli cells permeabilized with organic solvents." PROCESS BIOCHEMISTRY, vol. 39, no. 3, 28 November 2003 (2003-11-28), pages 301-305, XP008093218 ISSN: 1359-5113 cited in the application
  • PRABHUNE A ET AL: "IMMOBILIZATION OF PENICILLIN ACYLASE IN POROUS BEADS OF POLYACRYLAMIDE GEL" APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 30, no. 3, 1991, pages 265-272, XP008093240 ISSN: 0273-2289
 
Remarks:
The file contains technical information submitted after the application was filed and not included in this specification
 
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).