(19)
(11) EP 2 186 886 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
12.06.2013 Bulletin 2013/24

(45) Mention of the grant of the patent:
08.08.2012 Bulletin 2012/32

(21) Application number: 09004164.1

(22) Date of filing: 18.01.2006
(27) Previously filed application:
 18.01.2006 PCT/JP2006/300606
(51) International Patent Classification (IPC): 
C12N 9/20(2006.01)
C12P 7/64(2006.01)

(54)

Process for production of purified lipase

Verfahren zur Herstellung von gereinigter Lipase

Procédé de purification de lipase


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

(30) Priority: 19.01.2005 JP 2005011770
19.01.2005 JP 2005011771

(43) Date of publication of application:
19.05.2010 Bulletin 2010/20

(62) Application number of the earlier application in accordance with Art. 76 EPC:
06711875.2 / 1842908

(73) Proprietor: The Nisshin OilliO Group, Ltd.
Tokyo 104-8285 (JP)

(72) Inventors:
  • Negishi, Satoshi
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Suzuki, Junko
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Sakurai, Chika
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Arimoto, Shin
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Uehara, Hidetaka
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Hirose, Tadashiro
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Yamauchi, Yoshie
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Arai, Yuri
    Yokosuka-shi Kanagawa 239-0832 (JP)
  • Suganuma, Tomomi
    Yokosuka-shi Kanagawa 239-0832 (JP)

(74) Representative: Bawden, Peter Charles 
Bawden & Associates 4 The Gatehouse 2 High Street
Harpenden, Hertfordshire AL5 2TH
Harpenden, Hertfordshire AL5 2TH (GB)


(56) References cited: : 
EP-A- 1 795 591
JP-A- 8 089 265
WO-A-90/04033
   
  • MOGI K-I ET AL: "Transesterification reaction between medium- and long-chain fatty acid triglycerides using surfactant-modified lipase" BIOTECHNOLOGY AND BIOENGINEERING, WILEY & SONS, HOBOKEN, NJ, US, vol. 67, no. 5, 5 March 2000 (2000-03-05), pages 513-519, XP003004057 ISSN: 0006-3592
  • TAKAHASHI K ET AL: "LIPASE MADE ACTIVE IN HYDROPHOBIC MEDIA" 1988, JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, VOL. 65, NR. 6, PAGE(S) 911-916 , XP002570823 ISSN: 0003-021X * page 915 - page 916; table 8 *
  • NEGISHI SATOSHI ET AL: "Activation of powdered lipase by cluster water and the use of lipase powders for commercial esterification of food oils" ENZYME AND MICROBIAL TECHNOLOGY, STONEHAM, MA, US, vol. 32, no. 1, 2 January 2003 (2003-01-02), pages 66-70, XP002456965 ISSN: 0141-0229
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).