(19)
(11) EP 2 192 194 B8

(12) CORRECTED EUROPEAN PATENT SPECIFICATION
Note: Bibliography reflects the latest situation

(15) Correction information:
Corrected version no 1 (W1 B1)

(48) Corrigendum issued on:
24.06.2015 Bulletin 2015/26

(45) Mention of the grant of the patent:
20.05.2015 Bulletin 2015/21

(21) Application number: 10150793.7

(22) Date of filing: 04.12.2002
(51) International Patent Classification (IPC): 
C12Q 1/68(2006.01)
C07H 21/02(2006.01)
C12P 19/34(2006.01)
C07H 21/04(2006.01)

(54)

Disease detection by digital protein truncation assays

Krankheitsnachweis mit digitalen Proteinverkürzungstests

Détection d'une maladie par des analyses numériques de troncation des protéines


(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SI SK TR

(30) Priority: 06.12.2001 US 336177 P

(43) Date of publication of application:
02.06.2010 Bulletin 2010/22

(60) Divisional application:
15168129.3

(62) Application number of the earlier application in accordance with Art. 76 EPC:
02808038.0 / 1523573

(73) Proprietor: The Johns Hopkins University
Baltimore, MD 21201 (US)

(72) Inventors:
  • Traverso, Giovanni, C
    Cambridge, MA 02139 (CA)
  • Kinzler, Kenneth, W.
    Baltimore, MD 21230 (US)
  • Vogelstein, Bert
    Baltimore, MD 21208 (US)

(74) Representative: Tombling, Adrian George 
Withers & Rogers LLP 4 More London Riverside
London SE1 2AU
London SE1 2AU (GB)


(56) References cited: : 
WO-A-01/09386
US-A- 5 709 998
   
  • VOGELSTEIN B. ET AL.: "Digital PCR" PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, WASHINGTON, DC, US, vol. 96, no. 16, 3 August 1999 (1999-08-03), pages 9236-9241, XP002185144 ISSN: 0027-8424
  • ROMEY MARIE-CATHERINE ET AL: "Transcript analysis of CFTR frameshift mutations in lymphocytes using the reverse transcription-polymerase chain reaction technique and the protein truncation test" HUMAN GENETICS, vol. 98, no. 3, 1996, pages 328-332, XP009131861 ISSN: 0340-6717
  • ZHOU WEI ET AL: "Counting alleles reveals a connection between chromosome 18q loss and vascular invasion" January 2001 (2001-01), NATURE BIOTECHNOLOGY, VOL. 19, NR. 1, PAGE(S) 78-81 , XP002580682 ISSN: 1087-0156 * abstract; figures 1-3 *
  • POWELL S.M. ET AL.: "MOLECULAR DIAGNOSIS OF FAMILIAL ADENOMATOUS POLYPOSIS" NEW ENGLAND JOURNAL OF MEDICINE, THE, MASSACHUSETTS MEDICAL SOCIETY, MA, US, vol. 329, no. 27, 30 December 1993 (1993-12-30), pages 1982-1987, XP009010962 ISSN: 0028-4793
  • DUNNEN DEN J.T. ET AL.: "THE PROTEIN TRUNCATION TEST: A REVIEW" HUMAN MUTATION, WILEY-LISS, NEW YORK, NY, US, vol. 14, no. 2, 1999, pages 95-102, XP009006085 ISSN: 1059-7794
   
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).