FIELD OF THE INVENTION
[0001] The present invention relates,
inter alia, to novel variants (mutants) of parent Termamyl-like α-amylases, notably variants
exhibiting increased thermostability at acidic pH and/or at low Ca
2+ concentrations (relative to the parent) which are advantageous with respect to applications
of the variants in, industrial starch processing particularly (
e.
g. starch liquefaction or saccharification).
BACKGROUND OF THE INVENTION
[0002] α-Amylases (α-1,4-glucan-4-glucanohydrolases, EC 3.2.1.1) constitute a group of enzymes
which catalyze hydrolysis of starch and other linear and branched 1,4-glucosidic oligo-
and polysaccharides.
[0003] There is a very extensive body of patent and scientific literature relating to this
industrially very important class of enzymes. A number of α-amylase such as Termamyl-like
α-amylases variants are known from
e.
g.
WO 90/11352,
WO 95/10603,
WO 95/26397,
WO 96/23873 and
WO 96/23874.
[0004] Among more recent disclosures relating to α-amylases,
WO 96/23874 provides three-dimensional, X-ray crystal structural data for a Termamyl-like α-amylase
which consists of the 300 N-terminal amino acid residues of the
B. amyloliquefaciens α-amylase and amino acids 301-483 of the C-terminal end of the
B. licheniformis α-amylase comprising the amino acid sequence (the latter being available commercially
under the tradename Termamyl
™), and which is thus closely related to the industrially important
Bacillus α-amylases (which in the present context are embraced within the meaning of the term
"Termamyl-like α-amylases", and which include,
inter alia, the
B. licheniformis, B. amyloliquefaciens and
B. stearothermophilus α-amylases).
WO 96/23874 further describes methodology for designing, on the basis of an analysis of the structure
of a parent Termamyl-like α-amylase, variants of the parent Termamyl-like α-amylase
which exhibit altered properties relative to the parent.
[0005] WO 95/35382 (Gist Brocades B.V.) concerns amylolytic enzymes derived from
B. licheniformis with improved properties allowing reduction of the Ca
2+ concentration under application without a loss of performance of the enzyme. The
amylolytic enzyme comprises one or more amino acid changes at positions selected from
the group of 104, 128, 187, 188 of the
B. licheniformis α-amylase sequence.
[0006] WO 96/23873 (Novo Nordisk) discloses Termamyl-like α-amylase variants which have increased thermostability
obtained by pairwise deletion in the region R181*, G182*, T183* and G184* of the sequence
shown in SEQ ID NO: 1 herein.
BRIEF DISCLOSURE OF THE INVENTION
[0007] The present invention relates to novel α-amylolytic variants (mutants) of a Termamyl-like
α-amylase, in particular variants exhibiting increased thermostability (relative to
the parent) which are advantageous in connection with the industrial processing of
starch (starch liquefaction, saccharification and the like).
[0008] The inventors have surprisingly found out that in case of combining two, three, four,
five or six mutations (will be described below), the thermostability of Termamyl-like
α-amylases is increased at acidic pH and/or at low Ca
2+ concentration in comparison to single mutations, such as the mutation dislcosed in
WO 96/23873 (Novo Nordisk),
i.
e. pairwise deletion in the region R181*, G182*, T183* and G184* of the sequence shown
in SEQ ID NO: 1 herein.
[0009] The invention further relates to DNA constructs encoding variants of the invention,
to composition comprising variants of the invention, to methods for preparing variants
of the invention, and to the use of variants and compositions of the invention, alone
or in combination with other α-amylolytic enzymes, in various industrial processes,
e.
g., starch liquefaction.
BRIEF DESCRIPTION OF THE DRAWING
[0010]
Figure 1 is an alignment of the amino acid sequences of six parent Termamyl-like α-amylases
in the context of the invention. The numbers on the Extreme left designate the respective
amino acid sequences as follows:
1: SEQ ID NO: 2,
2: Kaoamyl,
3: SEQ ID NO: 1,
4: SEQ ID NO: 5,
5: SEQ ID NO: 4,
6: SEQ ID NO: 3.
DETAILED DISCLOSURE OF THE INVENTION
The Termamyl-like α-amlase
[0011] It is well known that a number of α-amylases produced by
Bacillus spp. are highly homologous on the amino acid level. For instance, the
B. licheniformis α-amylase comprising the amino acid sequence shown in SEQ ID NO: 4 (commercially
available as Termamyl
™) has been found to be about 89% homologous with the
B. amyloliquefaciens α-amylase comprising the amino acid sequence shown in SEQ ID NO: 5 and about 79%
homologous with the
B. stearothermophilus α-amylase comprising the amino acid sequence shown in SEQ ID NO: 3. Further homologous
α-amylases include an α-amylase derived from a strain of the
Bacillus sp. NCIB 12289, NCIB 12512, NCIB 12513 or DSM 9375, all of which are described in
detail in
WO 95/26397, and the α-amylase described by
Tsukamoto et al., Biochemical and Biophysical Research Communications, 151 (1988),
pp. 25-31.
[0012] Still further homologous α-amylases include the α-amylase produced by the
B. licheniformis strain described in
EP 0252666 (ATCC 27811), and the α-amylases identified in
WO 91/00353 and
WO 94/18314. Other commercial Termamyl-like
B. licheniformis α-amylases are Optitherm
™ and Takatherm
™ (available from Solvay), Maxamyl
™ (available from Gist-brocades/Genencor), Spezym AA
™ and Spezyme Delta AA
™ (available from Genencor), and Keistase
™ (available from Daiwa).
[0013] Because of the substantial homology found between these α-amylases, they are considered
to belong to the same class of α-amylases, namely the class of "Termamyl-like α-amylases".
[0014] Accordingly, in the present context, the term "Termamyl-like α-amylase" is intended
to indicate an α-amylase which, at the amino acid level, exhibits a substantial homology
to Termamyl
™,
i.
e. the
B. licheniformis α-amylase having the amino acid sequence shown in SEQ ID NO: 4 herein. In other words,
a Termamyl-like α-amylase is an α-amylase which has the amino acid sequence shown
in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7 or 8 herein, and the amino acid sequence shown
in SEQ ID NO: 1 of
WO 95/26397 (the same as the amino acid sequence shown as SEQ ID NO: 7 herein) or in SEQ ID NO:
2 of
WO 95/26397 (the same as the amino acid sequence shown as SEQ ID NO: 8 herein) or in Tsukamoto
et al., 1988, (which amino acid sequence is shown in SEQ ID NO: 6 herein) or i) which
displays at least 60%, preferred at least 70%, more preferred at least 75%, even more
preferred at least 80%, especially at least 85%, especially preferred at least 90%,
even especially more preferred at least 95% homology with at least one of said amino
acid sequences shown in SEQ ID NOS 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 and/or ii)
displays immunological cross-reactivity with an antibody raised against at least one
of said α-amylases, and/or iii) is encoded by a DNA sequence which hybridizes to the
DNA sequences encoding the above-specified α-amylases which are apparent from SEQ
ID NOS: 9, 10, 11, or 12 of the present application (which encoding sequences encode
the amino acid sequences shown in SEQ ID NOS: 1, 2, 3, 4 and 5 herein, respectively),
from SEQ ID NO: 4 of
WO 95/26397 (which DNA sequence, together with the stop codon TAA, is shown in SEQ ID NO: 13
herein and encodes the amino acid sequence shown in SEQ ID NO: 8 herein) and from
SEQ ID NO: 5 of
WO 95/26397 (shown in SEQ ID NO: 14 herein), respectively.
[0015] In connection with property i), the "homology" may be determined by use of any conventional
algorithm, preferably by use of the GAP progamme from the GCG package version 7.3
(June 1993) using default values for GAP penalties, which is a GAP creation penalty
of 3.0 and GAP extension penalty of 0.1, (Genetic Computer Group (1991) Programme
Manual for the GCG Package, version 7, 575 Science Drive, Madison, Wisconsin, USA
53711).
[0017] Property ii) of the α-amylase,
i.
e. the immunological cross reactivity, may be assayed using an antibody raised against,
or reactive with, at least one epitope of the relevant Termamyl-like α-amylase. The
antibody, which may either be monoclonal or polyclonal, may be produced by methods
known in the art,
e.
g. as described by
Hudson et al., Practical Immunology, Third edition (1989), Blackwell Scientific Publications. The immunological cross-reactivity may be determined using assays known in the art,
examples of which are Western Blotting or radial immunodiffusion assay,
e.
g. as described by Hudson et al., 1989. In this respect, immunological cross-reactivity
between the α-amylases having the amino acid sequences SEQ ID NOS: 1, 2, 3, 4, 5,
6, 7, or 8 respectively, have been found.
[0018] The oligonucleotide probe used in the characterization of the Termamyl-like α-amylase
in accordance with property iii) above may suitably be prepared on the basis of the
full or partial nucleotide or amino acid sequence of the α-amylase in question.
[0019] Suitable conditions for testing hybridization involve presoaking in 5xSSC and prehybridizing
for 1 hour at ∼40°C in a solution of 20% formamide, 5xDenhardt's solution, 50mM sodium
phosphate, pH 6.8, and 50mg of denatured sonicated calf thymus DNA, followed by hybridization
in the same solution supplemented with 100mM ATP for 18 hours at ∼40°C, followed by
three times washing of the filter in 2xSSC, 0.2% SDS at 40°C for 30 minutes (low stringency),
preferred at 50°C (medium stringency), more preferably at 65°C (high stringency),
even more preferably at ∼75°C (very high stringency). More details about the hybridization
method can be found in
Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor,
1989.
[0020] In the present context, "derived from" is intended not only to indicate an α-amylase
produced or producible by a strain of the organism in question, but also an α-amylase
encoded by a DNA sequence isolated from such strain and produced in a host organism
transformed with said DNA sequence. Finally, the term is intended to indicate an α-amylase
which is encoded by a DNA sequence of synthetic and/or cDNA origin and which has the
identifying characteristics of the α-amylase in question. The term is also intended
to indicate that the parent α-amylase may be a variant of a naturally occurring α-amylase,
i.e. a variant which is the result of a modification (insertion, substitution, deletion)
of one or more amino acid residues of the naturally occurring α-amylase.
Parent hybrid α-amylases
[0021] The parent α-amylase may be a hybrid α-amylase, i.e. an α-amylase which comprises
a combination of partial amino acid sequences derived from at least two α-amylases.
[0022] The parent hybrid α-amylase may be one which on the basis of amino acid homology
and/or immunological cross-reactivity and/or DNA hybridization (as defined above)
can be determined to belong to the Termamyl-like α-amylase family. In this case, the
hybrid α-amylase is typically composed of at least one part of a Termamyl-like α-amylase
and part(s) of one or more other α-amylases selected from Termamyl-like α-amylases
or non-Termamyl-like α-amylases of microbial (bacterial or fungal) and/or mammalian
origin.
[0023] Thus, the parent hybrid α-amylase may comprise a combination of partial amino acid
sequences deriving from at least two Termamyl-like α-amylases, or from at least one
Termamyl-like and at least one non-Termamyl-like bacterial α-amylase, or from at least
one Termamyl-like and at least one fungal α-amylase. The Termamyl-like α-amylase from
which a partial amino acid sequence derives may,
e.
g., be any of those specific Termamyl-like α-amylases referred to herein.
[0024] For instance, the parent α-amylase may comprise a C-terminal part of an α-amylase
derived from a strain of
B. licheniformis, and a N-terminal part of an α-amylase derived from a strain of
B. amyloliquefaciens or from a strain of
B. stearothermophilus. For instance, the parent α-amylase may comprise at least 430 amino acid residues
of the C-terminal part of the
B. licheniformis α-amylase, and may,
e.
g. comprise a) an amino acid segment corresponding to the 37 N-terminal amino acid
residues of the
B. amyloliquefaciens α-amylase having the amino acid sequence shown in SEQ ID NO: 5 and an amino acid
segment corresponding to the 445 C-terminal amino acid residues of the
B. licheniformis α-amylase having the amino acid sequence shown in SEQ ID No. 4, or b) an amino acid
segment corresponding to the 68 N-terminal amino acid residues of the
B. stearothermophilus α-amylase having the amino acid sequence shown in SEQ ID NO: 3 and an amino acid
segment corresponding to the 415 C-terminal amino acid residues of the
B. licheniformis α-amylase having the amino acid sequence shown in SEQ ID NO: 4.
[0025] The non-Termamyl-like α-amylase may,
e.
g., be a fungal α-amylase, a mammalian or a plant α-amylase or a bacterial α-amylase
(different from a Termamyl-like α-amylase). Specific examples of such α-amylases include
the
Aspergillus oryzae TAKA α-amylase, the
A. niger acid α-amylase, the
Bacillus subtilis α-amylase, the porcine pancreatic α-amylase and a barley α-amylase. All of these
α-amylases have elucidated structures which are markedly different from the structure
of a typical Termamyl-like α-amylase as referred to herein.
[0026] The fungal α-amylases mentioned above, i.e. derived from
A. niger and
A. oryzae, are highly homologous on the amino acid level and generally considered to belong
to the same family of α-amylases. The fungal α-amylase derived from
Aspergillus oryzae is commercially available under the tradename Fungamyl
™.
[0027] Furthermore, when a particular variant of a Termamyl-like α-amylase (variant of the
invention) is referred to - in a conventional manner - by reference to modification
(
e.
g. deletion or substitution) of specific amino acid residues in the amino acid sequence
of a specific Termamyl-like α-amylase, it is to be understood that variants of another
Termamyl-like α-amylase modified in the equivalent position(s) (as determined from
the best possible amino acid sequence alignment between the respective amino acid
sequences) are encompassed thereby.
[0028] A preferred embodiment of a variant of the invention is one derived from a
B. licheniformis α-amylase (as parent Termamyl-like α-amylase), e.g. one of those referred to above,
such as the
B. licheniformis α-amylase having the amino acid sequence shown in SEQ ID NO: 4.
Construction of variants of the invention
[0029] The construction of the variant of interest may be accomplished by cultivating a
microorganism comprising a DNA sequence encoding the variant under conditions which
are conducive for producing the variant. The variant may then subsequently be recovered
from the resulting culture broth. This is described in detail further below.
Altered properties of variants of the invention
[0030] The following discusses the relationship between mutations which may be present in
variants of the invention, and desirable alterations in properties (relative to those
a parent, Termamyl-like α-amylase) which may result therefrom.
Increased thermostability at acidic pH and/or at low Ca2+ concentration
[0031] Mutations of particular relevance in relation to obtaining variants according to
the invention having increased thermostability at acidic pH and/or at low Ca
2+ concentration include mutations at the following positions (relative to
B. licheniformis α-amylase, SEQ ID NO: 4):
H156, N172, A181, N188, N190, H205, D207, A209, A210, E211, Q264, N265.
[0032] In the context of the invention the term "acidic pH" means a pH below 7.0, especially
below the pH range, in which industrial starch liquefaction processes are normally
performed, which is between pH 5.5 and 6.2.
[0033] In the context of the present invention the term "low Calcium concentration" means
concentrations below the normal level used in industrial starch liquefaction. Normal
concentrations vary depending of the concentration of free Ca
2+ in the corn. Normally a dosage corresponding to 1mM (40ppm) is added which together
with the level in corn gives between 40 and 60ppm free Ca
2+ .
[0034] In the context of the invention the term "high tempertatures" means temperatures
between 95°C and 160°C, especially the temperature range in which industrial starch
liquefaction processes are normally performed, which is between 95°C and 105°C.
[0035] The inventors have now found that the thermostability at acidic pH and/or at low
Ca
2+ concentration may be increased even more by combining certain mutations including
the above mentioned mutations and/or I201 with each other.
[0036] Said "certain" mutations are the following (relative to
B. licheniformis α-amylase, SEQ ID NO: 4):
N190, D207, E211, Q264 and I201.
[0037] Said mutation may further be combined with deletions in one, preferably two or even
three positions as described in
WO 96/23873 (
i.
e. in positions R181, G182, T183, G184 in SEQ ID NO: 1 herein). According to the invention
variants of a parent Termamyl-like α-amylase with α-amylase activity comprising mutations
in two, three, four, five or six of the above positions are contemplated.
[0038] It should be emphazised that not only the Termamyl-like α-amylases mentioned specifically
below are contemplated. Also other commercial Termamyl-like α-amylases are contemplated.
An unexhaustive list of such α-amylases is the following:
α-amylases produced by the B. licheniformis strain described in EP 0252666 (ATCC 27811), and the α-amylases identified in WO 91/00353 and WO 94/18314. Other commercial Termamyl-like B. licheniformis α-amylases are Optitherm™ and Takatherm™ (available from Solvay), Maxamyl™ (available from Gist-brocades/Genencor), Spezym AA™ Spezyme Delta AA™ (available from Genencor), and Keistase™ (available from Daiwa).
[0039] It may be mentioned here that amino acid residues, respectively, at positions corresponding
to N190, I201, D207 and E211, respectively, in SEQ ID NO: 4 constitute amino acid
residues which are conserved in numerous Termamyl-like α-amylases. Thus, for example,
the corresponding positions of these residues in the amino acid sequences of a number
of Termamyl-like α-amylases which have already been mentioned (
vide supra) are as follows:
Table 1.
Termamyl-like α-amylase |
N |
I |
D |
E |
Q |
B. licheniformis (SEQ ID NO: 4) |
N190 |
I201 |
D207 |
E211 |
Q264 |
B. amyloliquefaciens (SEQ ID NO: 5) |
N190 |
V201 |
D207 |
E211 |
Q264 |
B. stearothermophilus (SEQ ID NO: 3) |
N193 |
L204 |
E210 |
E214 |
--- |
Bacillus WO 95/26397 (SEQ ID NO: 2) |
N195 |
V206 |
E212 |
E216 |
--- |
Bacillus WO 95/26397 (SEQ ID NO: 1) |
N195 |
V206 |
E212 |
E216 |
--- |
"Bacillus sp. #707" (SEQ ID NO: 6) |
N195 |
I206 |
E212 |
E216 |
--- |
[0040] Mutations of these conserved amino acid residues are very important in relation to
improving thermostability at acidic pH and/or at low calcium concentration, and the
following mutations are of particular interest in this connection (with reference
to the numbering of the
B. licheniformis amino acid sequence shown in SEQ ID NO: 4).
[0041] Pair-wise amino acid deletions at positions corresponding to R179-G182 in SEQ ID
NO: 5 corresponding to a gap in Seq ID NO: 4. when aligned with a numerous Termamyl-like
α-amylases. Thus, for example, the corresponding positions of these residues in the
amino acid sequences of a number of Termamyl-like α-amylases which have already been
mentioned (
vide supra) are as follows:
Table 2.
Termamyl-like α-amylase |
Pair wise amino acid deletions among |
B. amyloliquefaciens (SEQ ID No.5) |
R176, G177, E178, G179 |
B. stearothermophilus (SEQ ID No.3) |
R179, G180, I181, G182 |
Bacillus WO 95/26397 (SEQ ID No.2) |
R181, G182, T183, G184 |
Bacillus WO 95/26397 (SEQ ID No.1) |
R181, G182, D183, G184 |
"Bacillus sp. #707" (SEQ ID No.6) |
R181, G182, H183, G184 |
[0042] When using SEQ ID NO: 1 to SEQ ID NO: 6 as the backbone (i.e. as the parent Termamyl-like
α-amylase) two, three, four, five or six mutations may according to the invention
be made in the following regions/positions to increase the thermostability at acidic
pH and/or at low Ca
2+ concentrations (relative to the parent):
(relative to Seq ID NO: 1 herein):
1: R181*, G182*, T183*, G184*
2: N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
3: V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
4: E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
5: E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
6: K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
(relative to SEQ ID NO: 2 herein):
1: R181*,G182*,D183*,G184*
2: N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
3: V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
4: E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
5: E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
6: K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
(Relative to SEQ ID NO: 3 herein):
1: R179*,G180,1181*,G182*
2: N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
3: L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V;
4: E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
5: E214A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
6: S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V
Relative to SEQ ID NO: 4 herein):
1: Q178*,G179*
2: N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
3: I201A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
4: D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
5: E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
6: Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
(relative to SEQ ID NO: 5 herein):
1: R176*,G177*,E178,G179*
2: N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
3: V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
4: D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
5: E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
6: Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
(relative to SEQ ID NO: 6 herein):
1: R181*,G182*,H183*,G184*
2: N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
3: I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
4: E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
5: E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
6: K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V.
[0043] Comtemplated according to the present invention is combining three, four, five or
six mutation.
[0044] Specific double mutations for backbone SEQ ID NO: 1 to SEQ ID NO: 6 are listed in
the following.
[0045] Using SEQ ID NO: 1 as the backbone the following double mutantions resulting in the
desired effect are comtemplated according to the invention:
-R181*/G182*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/T183*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-T183*/G184*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-G182*/T183*/V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-T183*/G184*/V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-R181*/G182*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/T183*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-T183*/G184*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V:
-R181*/G182*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/T183*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-T183*/G184*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-G182*/T183*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-T183*/G184*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
[0046] Using SEQ ID NO: 2 as the backbone the following double mutantions resulting in the
desired effect are comtemplated according to the invention:
-R181*/G182*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/D183*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-D183*/G184*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-G182*/T183*/V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-T183*/G184*/V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-R181*/G182*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/T183*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-T183*/G184*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/T183*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-T183*/G184*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-G182*/T183*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-T183*/G184*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-N195 A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-V206A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
[0047] Using SEQ ID NO. 3 as the backbone the following double mutantions resulting in the
desired effect are comtemplated according to the invention:
-R179*/G180*/N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G180*/I181*/N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I181*/G182*/N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R179*/G180*/L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V;
-G180*/I181*/L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V;
-I181*/G182*/L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V;
-R179*/G180*/E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G180*/I181*/E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I181*/G182*/E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R179*/G180*/E214A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G180*/I181*/E214A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I181*/G182*/E214A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R179*/G180*/S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V;
-G180*/I181*/S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V;
-I181*/G182*/S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V;
-N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V;
-N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E214A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N193A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V;
-L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V /E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V /E214A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-L204A,R,D,N,C,E,Q,G,H,I,K,M,F,P,S,T,W,Y,V /S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V;
-E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E210A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V;
-E214A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /S267A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,T,W,Y,V;
[0048] Using SEQ ID NO. 4 as the backbone the following double mutantions resulting in the
desired effect are comtemplated according to the invention:
-Q178*/G179*/N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-Q178*/G179*/I201A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
-Q178*/G179*/D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-Q178*/G179*/E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R179*/G180*/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N190/I201A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
-N190/D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N190/E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N190/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I201/D207A,R,N,C,Q,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I201/E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I201/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-D207/E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-D207/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E211/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
[0049] Using SEQ ID NO: 5 as the backbone the following double mutantions resulting in the
desired effect are comtemplated according to the invention:
-R176*/G177*/N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G177*/E178*/N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E178*/G179*/N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R176*/G177*/V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-G176*/E178*/V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-E178*/G179*/V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-R176*/G177*/D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G177*/E178*/D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E178*/G179*/D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R176*/G177*/E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G177*/E178*/E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E178*/G179*/E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R176*/G177*/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G177*/E178*/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E178*/G179*/Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y;
-N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N190A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-V201A,R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y /Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-D207A,R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E211A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /Q264A,R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V.
[0050] Using SEQ ID NO: 6 as the backbone the following double mutantions resulting in the
desired effect are comtemplated according to the invention:
-R181*/G182*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/H183*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-H183*/G184*/N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
-G182*/H183*/I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
-H183*/G184*/I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/H183*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-H183*/G184*/E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-G182*/H183*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-H183*/G184*/E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-R181*/G182*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-G182*/H183*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-H183*/G184*/K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-N195A,R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V /E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-I206A,R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V;
-E212A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
-E216A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V /K269A,R,D,N,C,E,Q,G,H,I,L,M,F,P,S,T,W,Y,V;
[0051] All Termamyl-like α-amylase defined above may suitably be used as backbone for preparing
variants of the invention.
[0052] However, in a preferred embodiment the variant comprises the following mutations:
N190F/Q264S in SEQ ID NO: 4 or in corresponding positiones in another parent Termamyl-like
α-amylases.
[0053] In another embodiment the variant of the invention comprises the following mutations:
I181*/G182*/N193F in SEQ ID NO: 3 (TVB146) or in corresponding positions in another
parent Termamyl-like α-amylases. Said variant may further comprise a substitution
in position E214Q.
[0054] In a preferred embodiment of the invention the parent Termamyl-like α-amylase is
a hybrid α-amylase of SEQ ID NO: 4 and SEQ ID NO: 5. Specifically, the parent hybrid
Termamyl-like α-amylase may be a hybrid alpha-amylase comprising the 445 C-terminal
amino acid residues of the
B. licheniformis α-amylase shown in SEQ ID NO: 4 and the 37 N-terminal amino acid residues of the
α-amylase derived from
B. amyloliquefaciens shown in SEQ ID NO: 5, which may suitably further have the following mutations: H156Y+A181T+N190F+A209V+Q264S
(using the numbering in SEQ ID NO: 4). The latter mentioned hybrid is used in the
examples below and is referred to as LE174.
General mutations in variants of the invention
[0055] It may be preferred that a variant of the invention comprises one or more modifications
in addition to those outlined above. Thus, it may be advantageous that one or more
proline residues present in the part of the α-amylase variant which is modified is/are
replaced with a non-proline residue which may be any of the possible, naturally occurring
non-proline residues, and which preferably is an alanine, glycine, serine, threonine,
valine or leucine.
[0056] Analogously, it may be preferred that one or more cysteine residues present among
the amino acid residues with which the parent α-amylase is modified is/are replaced
with a non-cysteine residue such as serine, alanine, threonine, glycine, valine or
leucine.
[0057] Furthermore, a variant of the invention may - either as the only modification or
in combination with any of the above outlined modifications - be modified so that
one or more Asp and/or Glu present in an amino acid fragment corresponding to the
amino acid fragment 185-209 of SEQ ID NO: 4 is replaced by an Asn and/or Gln, respectively.
Also of interest is the replacement, in the Termamyl-like α-amylase, of one or more
of the Lys residues present in an amino acid fragment corresponding to the amino acid
fragment 185-209 of SEQ ID NO: 4 by an Arg.
[0058] It will be understood that the present invention encompasses variants incorporating
two or more of the above outlined modifications.
[0059] Furthermore, it may be advantageous to introduce point-mutations in any of the variants
described herein.
Methods for preparing α-amylase variants
[0060] Several methods for introducing mutations into genes are known in the art. After
a brief discussion of the cloning of α-amylase-encoding DNA sequences, methods for
generating mutations at specific sites within the α-amylase-encoding sequence will
be discussed.
Cloning a DNA sequence encoding an α-amylase
[0061] The DNA sequence encoding a parent α-amylase may be isolated from any cell or microorganism
producing the α-amylase in question, using various methods well known in the art.
First, a genomic DNA and/or cDNA library should be constructed using chromosomal DNA
or messenger RNA from the organism that produces the α-amylase to be studied. Then,
if the amino acid sequence of the α-amylase is known, homologous, labelled oligonucleotide
probes may be synthesized and used to identify α-amylase-encoding clones from a genomic
library prepared from the organism in question. Alternatively, a labelled oligonucleotide
probe containing sequences homologous to a known α-amylase gene could be used as a
probe to identify α-amylase-encoding clones, using hybridization and washing conditions
of lower stringency.
[0062] Yet another method for identifying α-amylase-encoding clones would involve inserting
fragments of genomic DNA into an expression vector, such as a plasmid, transforming
α-amylase-negative bacteria with the resulting genomic DNA library, and then plating
the transformed bacteria onto agar containing a substrate for α-amylase, thereby allowing
clones expressing the α-amylase to be identified.
[0063] Alternatively, the DNA sequence encoding the enzyme may be prepared synthetically
by established standard methods, e.g. the phosphoroamidite method described by S.L.
Beaucage and M.H. Caruthers (1981) or the method described by Matthes et al. (1984).
In the phosphoroamidite method, oligonucleotides are synthesized, e.g. in an automatic
DNA synthesizer, purified, annealed, ligated and cloned in appropriate vectors.
[0064] Finally, the DNA sequence may be of mixed genomic and synthetic origin, mixed synthetic
and cDNA origin or mixed genomic and cDNA origin, prepared by ligating fragments of
synthetic, genomic or cDNA origin (as appropriate, the fragments corresponding to
various parts of the entire DNA sequence), in accordance with standard techniques.
The DNA sequence may also be prepared by polymerase chain reaction (PCR) using specific
primers, for instance as described in
US 4,683,202 or R.K. Saiki et al. (1988).
Site-directed mutagenesis
[0065] Once an α-amylase-encoding DNA sequence has been isolated, and desirable sites for
mutation identified, mutations may be introduced using synthetic oligonucleotides.
These oligonucleotides contain nucleotide sequences flanking the desired mutation
sites; mutant nucleotides are inserted during oligonucleotide synthesis. In a specific
method, a single-stranded gap of DNA, bridging the α-amylase-encoding sequence, is
created in a vector carrying the α-amylase gene. Then the synthetic nucleotide, bearing
the desired mutation, is annealed to a homologous portion of the single-stranded DNA.
The remaining gap is then filled in with DNA polymerase I (Klenow fragment) and the
construct is ligated using T4 ligase. A specific example of this method is described
in
Morinaga et al. (1984). US 4,760,025 discloses the introduction of oligonucleotides encoding multiple mutations by performing
minor alterations of the cassette. However, an even greater variety of mutations can
be introduced at any one time by the Morinaga method, because a multitude of oligonucleotides,
of various lengths, can be introduced.
[0066] Another method for introducing mutations into α-amylase-encoding DNA sequences is
described in Nelson and Long (1989). It involves the 3-step generation of a PCR fragment
containing the desired mutation introduced by using a chemically synthesized DNA strand
as one of the primers in the PCR reactions. From the PCR-generated fragment, a DNA
fragment carrying the mutation may be isolated by cleavage with restriction endonucleases
and reinserted into an expression plasmid.
Random Mutagenesis
[0067] Random mutagenesis is suitably performed either as localised or region-specific random
mutagenesis in at least three parts of the gene translating to the amino acid sequence
shown in question, or within the whole gene.
[0068] The random mutagenesis of a DNA sequence encoding a parent α-amylase may be conveniently
performed by use of any method known in the art.
[0069] In relation to the above, a further aspect of the present invention relates to a
method for generating a variant of a parent α-amylase, e.g. wherein the variant exhibits
altered or increased thermal stability relative to the parent, the method comprising:
- (a) subjecting a DNA sequence encoding the parent α-amylase to random mutagenesis,
- (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and
- (c) screening for host cells expressing anα-amylase variant which has an altered property
(i.e. thermal stability) relative to the parent α-amylase.
[0070] Step (a) of the above method of the invention is preferably performed using doped
primers.
[0071] For instance, the random mutagenesis may be performed by use of a suitable physical
or chemical mutagenizing agent, by use of a suitable oligonucleotide, or by subjecting
the DNA sequence to PCR generated mutagenesis. Furthermore, the random mutagenesis
may be performed by use of any combination of these mutagenizing agents. The mutagenizing
agent may,
e.
g., be one which induces transitions, transversions, inversions, scrambling, deletions,
and/or insertions.
Examples of a physical or chemical mutagenizing agent suitable for the present purpose
include ultraviolet (UV) ir-radiation, hydroxylamine, N-methyl-N'-nitro-N-nitrosoguanidine
(MNNG), O-methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium
bisulphite, formic acid, and nucleotide analogues. When such agents are used, the
mutagenesis is typically performed by incubating the DNA sequence encoding the parent
enzyme to be mutagenized in the presence of the mutagenizing agent of choice under
suitable conditions for the mutagenesis to take place, and selecting for mutated DNA
having the desired properties.
[0072] When the mutagenesis is performed by the use of an oligonucleotide, the oligonucleotide
may be doped or spiked with the three non-parent nucleotides during the synthesis
of the oligonucleotide at the positions which are to be changed. The doping or spiking
may be done so that codons for unwanted amino acids are avoided. The doped or spiked
oligonucleotide can be incorporated into the DNA encoding the α-amylase enzyme by
any published technique, using
e.
g. PCR, LCR or any DNA polymerase and ligase as deemed appropriate.
[0073] Preferably, the doping is carried out using "constant random doping", in which the
percentage of wild-type and mutation in each position is predefined. Furthermore,
the doping may be directed toward a preference for the introduction of certain nucleotides,
and thereby a preference for the introduction of one or more specific amino acid residues.
The doping may be made,
e.
g., so as to allow for the introduction of 90% wild type and 10% mutations in each
position. An additional consideration in the choice of a doping scheme is based on
genetic as well as protein-structural constraints. The doping scheme may be made by
using the DOPE program which,
inter alia, ensures that introduction of stop codons is avoided.
[0074] When PCR-generated mutagenesis is used, either a chemically treated or non-treated
gene encoding a parent α-amylase is subjected to PCR under conditions that increase
the mis-incorporation of nucleotides (Deshler 1992;
Leung et al., Technique, Vol.1, 1989, pp. 11-15).
[0075] A mutator strain of
E. coli (
Fowler et al., Molec. Gen. Genet., 133, 1974, pp. 179-191),
S. cereviseae or any other microbial organism may be used for the random mutagenesis of the DNA
encoding the α-amylase by,
e.
g., transforming a plasmid containing the parent glycosylase into the mutator strain,
growing the mutator strain with the plasmid and isolating the mutated plasmid from
the mutator strain. The mutated plasmid may be subsequently transformed into the expression
organism.
[0076] The DNA sequence to be mutagenized may be conveniently present in a genomic or cDNA
library prepared from an organism expressing the parent α-amylase. Alternatively,
the DNA sequence may be present on a suitable vector such as a plasmid or a bacteriophage,
which as such may be incubated with or other-wise exposed to the mutagenising agent.
The DNA to be mutagenized may also be present in a host cell either by being integrated
in the genome of said cell or by being present on a vector harboured in the cell.
Finally, the DNA to be mutagenized may be in isolated form. It will be understood
that the DNA sequence to be subjected to random mutagenesis is preferably a cDNA or
a genomic DNA sequence.
[0077] In some cases it may be convenient to amplify the mutated DNA sequence prior to performing
the expression step b) or the screening step c). Such amplification may be performed
in accordance with methods known in the art, the presently preferred method being
PCR-generated amplification using oligonucleotide primers prepared on the basis of
the DNA or amino acid sequence of the parent enzyme.
[0078] Subsequent to the incubation with or exposure to the mutagenising agent, the mutated
DNA is expressed by culturing a suitable host cell carrying the DNA sequence under
conditions allowing expression to take place. The host cell used for this purpose
may be one which has been transformed with the mutated DNA sequence, optionally present
on a vector, or one which was carried the DNA sequence encoding the parent enzyme
during the mutagenesis treatment. Examples of suitable host cells are the following:
gram positive bacteria such as
Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, Bacillus brevis, Bacillus
stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans,
Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis,
Streptomyces lividans or
Streptomyces murinus; and gram-negative bacteria such as
E. coli.
[0079] The mutated DNA sequence may further comprise a DNA sequence encoding functions permitting
expression of the mutated DNA sequence.
Localized random mutagenesis
[0080] The random mutagenesis may be advantageously localized to a part of the parent α-amylase
in question. This may,
e.
g., be advantageous when certain regions of the enzyme have been identified to be of
particular importance for a given property of the enzyme, and when modified are expected
to result in a variant having improved properties. Such regions may normally be identified
when the tertiary structure of the parent enzyme has been elucidated and related to
the function of the enzyme.
[0081] The localized, or region-specific, random mutagenesis is conveniently performed by
use of PCR generated mutagenesis techniques as described above or any other suitable
technique known in the art. Alternatively, the DNA sequence encoding the part of the
DNA sequence to be modified may be isolated,
e.
g., by insertion into a suitable vector, and said part may be subsequently subjected
to mutagenesis by use of any of the mutagenesis methods discussed above.
Alternative methods of providing α-amylase variants
[0082] Alternative methods for providing variants of the invention include gene shuffling
method known in the art including the methods
e.
g. described in
WO 95/22625 (from Affymax Technologies N.V.) and
WO 96/00343 (from Novo Nordisk A/S).
Expression of α-amylase variants
[0083] According to the invention, a DNA sequence encoding the variant produced by methods
described above, or by any alternative methods known in the art, can be expressed,
in enzyme form, using an expression vector which typically includes control sequences
encoding a promoter, operator, ribosome binding site, translation initiation signal,
and, optionally, a repressor gene or various activator genes.
[0084] The recombinant expression vector carrying the DNA sequence encoding an α-amylase
variant of the invention may be any vector which may conveniently be subjected to
recombinant DNA procedures, and the choice of vector will often depend on the host
cell into which it is to be introduced. Thus, the vector may be an autonomously replicating
vector, i.e. a vector which exists as an extrachromosomal entity, the replication
of which is independent of chromosomal replication, e.g. a plasmid, a bacteriophage
or an extrachromosomal element, minichromosome or an artificial chromosome. Alternatively,
the vector may be one which, when introduced into a host cell, is integrated into
the host cell genome and replicated together with the chromosome(s) into which it
has been integrated.
[0085] In the vector, the DNA sequence should be operably connected to a suitable promoter
sequence. The promoter may be any DNA sequence which shows transcriptional activity
in the host cell of choice and may be derived from genes encoding proteins either
homologous or heterologous to the host cell. Examples of suitable promoters for directing
the transcription of the DNA sequence encoding an α-amylase variant of the invention,
especially in a bacterial host, are the promoter of the
lac operon of
E.coli, the
Streptomyces coelicolor agarase gene
dagA promoters, the promoters of the
Bacillus licheniformis α-amylase gene (
amyL), the promoters of the
Bacillus stearothermophilus maltogenic amylase gene (
amyM), the promoters of the
Bacillus amyloliquefaciens α-amylase (
amyQ), the promoters of the
Bacillus subtilis xylA and xylB genes etc. For transcription in a fungal host, examples of useful promoters
are those derived from the gene encoding
A. oryzae TAKA amylase,
Rhizomucor miehei aspartic proteinase,
A. niger neutral α-amylase,
A. niger acid stable α-amylase,
A. niger glucoamylase,
Rhizomucor miehei lipase,
A. oryzae alkaline protease,
A. oryzae triose phosphate isomerase or
A. nidulans acetamidase.
[0086] The expression vector of the invention may also comprise a suitable transcription
terminator and, in eukaryotes, polyadenylation sequences operably connected to the
DNA sequence encoding the α-amylase variant of the invention. Termination and polyadenylation
sequences may suitably be derived from the same sources as the promoter.
[0087] The vector may further comprise a DNA sequence enabling the vector to replicate in
the host cell in question. Examples of such sequences are the origins of replication
of plasmids pUC19, pACYC177, pUB110, pE194, pAMB1 and pIJ702.
[0088] The vector may also comprise a selectable marker, e.g. a gene the product of which
complements a defect in the host cell, such as the
dal genes from
B. subtilis or
B. licheniformis, or one which confers antibiotic resistance such as ampicillin, kanamycin, chloramphenicol
or tetracyclin resistance. Furthermore, the vector may comprise
Aspergillus selection markers such as amdS, argB, niaD and sC, a marker giving rise to hygromycin
resistance, or the selection may be accomplished by co-transformation, e.g. as described
in
WO 91/17243.
[0089] While intracellular expression may be advantageous in some respects, e.g. when using
certain bacteria as host cells, it is generally preferred that the expression is extracellular.
In general, the
Bacillus α-amylases mentioned herein comprise a preregion permitting secretion of the expressed
protease into the culture medium. If desirable, this preregion may be replaced by
a different preregion or signal sequence, conveniently accomplished by substitution
of the DNA sequences encoding the respective preregions.
[0090] The procedures used to ligate the DNA construct of the invention encoding an α-amylase
variant, the promoter, terminator and other elements, respectively, and to insert
them into suitable vectors containing the information necessary for replication, are
well known to persons skilled in the art (cf., for instance,
Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor,
1989).
[0091] The cell of the invention, either comprising a DNA construct or an expression vector
of the invention as defined above, is advantageously used as a host cell in the recombinant
production of an α-amylase variant of the invention. The cell may be transformed with
the DNA construct of the invention encoding the variant, conveniently by integrating
the DNA construct (in one or more copies) in the host chromosome. This integration
is generally considered to be an advantage as the DNA sequence is more likely to be
stably maintained in the cell. Integration of the DNA constructs into the host chromosome
may be performed according to conventional methods,
e.
g. by homologous or heterologous recombination. Alternatively, the cell may be transformed
with an expression vector as described above in connection with the different types
of host cells.
[0092] The cell of the invention may be a cell of a higher organism such as a mammal or
an insect, but is preferably a microbial cell,
e.
g. a bacterial or a fungal (including yeast) cell.
[0093] Examples of suitable bacteria are grampositive bacteria such as
Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, Bacillus brevis, Bacillus
stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans,
Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, or
Streptomyces lividans or
Streptomyces murinus, or gramnegative bacteria such as
E.coli. The transformation of the bacteria may, for instance, be effected by protoplast
transformation or by using competent cells in a manner known per se.
[0094] The yeast organism may favourably be selected from a species of
Saccharomyces or
Schizosaccharomyces, e.g. Saccharomyces cerevisiae. The filamentous fungus may advantageously belong to a species of
Aspergillus, e.g. Aspergillus oryzae or
Aspergillus niger. Fungal cells may be transformed by a process involving protoplast formation and
transformation of the protoplasts followed by regeneration of the cell wall in a manner
known per se. A suitable procedure for transformation of
Aspergillus host cells is described in
EP 238 023.
[0095] In yet a further aspect, the present invention relates to a method of producing an
α-amylase variant of the invention, which method comprises cultivating a host cell
as described above under conditions conducive to the production of the variant and
recovering the variant from the cells and/or culture medium.
[0096] The medium used to cultivate the cells may be any conventional medium suitable for
growing the host cell in question and obtaining expression of the α-amylase variant
of the invention. Suitable media are available from commercial suppliers or may be
prepared according to published recipes (
e.
g. as described in catalogues of the American Type Culture Collection).
[0097] The α-amylase variant secreted from the host cells may conveniently be recovered
from the culture medium by well-known procedures, including separating the cells from
the medium by centrifugation or filtration, and precipitating proteinaceous components
of the medium by means of a salt such as ammonium sulphate, followed by the use of
chromatographic procedures such as ion exchange chromatography, affinity chromatography,
or the like.
Industrial applications
[0098] The α-amylase variants of this invention possesses valuable properties allowing for
a variety of industrial applications. In particular, enzyme variants of the invention
are applicable as a component in washing, dishwashing and hard-surface cleaning detergent
compositions. Numerous variants are particularly useful in the production of sweeteners
and ethanol from starch, and/or for textile desizing. Conditions for conventional
starch-conversion processes, including starch liquefaction and/or saccharification
processes, are described in,
e.
g.,
US 3,912,590 and in
EP patent publications Nos. 252 730 and
63 909.
Production of sweeteners from starch:
[0099] A "traditional" process for conversion of starch to fructose syrups normally consists
of three consecutive enzymatic processes, viz. a liquefaction process followed by
a saccharification process and an isomerization process. During the liquefaction process,
starch is degraded to dextrins by an α-amylase (
e.
g. Termamyl™) at pH values between 5.5 and 6.2 and at temperatures of 95-160°C for
a period of approx. 2 hours. In order to ensure an optimal enzyme stability under
these conditions, 1 mM of calcium is added (40 ppm free calcium ions).
[0100] After the liquefaction process the dextrins are converted into dextrose by addition
of a glucoamylase (
e.
g. AMG™) and a debranching enzyme, such as an isoamylase or a pullulanase (
e.
g. Promozyme™). Before this step the pH is reduced to a value below 4.5, maintaining
the high temperature (above 95°C), and the liquefying α-amylase activity is denatured.
The temperature is lowered to 60°C, and glucoamylase and debranching enzyme are added.
The saccharification process proceeds for 24-72 hours.
[0101] After the saccharification process the pH is increased to a value in the range of
6-8, preferably pH 7.5, and the calcium is removed by ion exchange. The dextrose syrup
is then converted into high fructose syrup using,
e.
g., an immmobilized glucoseisomerase (such as Sweetzyme™).
[0102] At least 1 enzymatic improvements of this process could be envisaged. Reduction of
the calcium dependency of the liquefying α-amylase. Addition of free calcium is required
to ensure adequately high stability of the α-amylase, but free calcium strongly inhibits
the activity of the glucoseisomerase and needs to be removed, by means of an expensive
unit operation, to an extent which reduces the level of free calcium to below 3-5
ppm. Cost savings could be obtained if such an operation could be avoided and the
liquefaction process could be performed without addition of free calcium ions.
[0103] To achieve that, a less calcium-dependent Termamyl-like α-amylase which is stable
and highly active at low concentrations of free calcium (< 40 ppm) is required. Such
a Termamyl-like α-amylase should have a pH optimum at a pH in the range of 4.5-6.5,
preferably in the range of 4.5-5.5.
Detergent compositions
[0104] As mentioned above, variants of the invention may suitably be incorporated in detergent
compositions. Increased thermostability at low calcium concentrations would be very
beneficial for amylase performance in detergents, i.e. the alkaline region. Reference
is made, for example, to
WO 96/23874 and
WO 97/07202 for further details concerning relevant ingredients of detergent compositions (such
as laundry or dishwashing detergents), appropriate methods of formulating the variants
in such detergent compositions, and for examples of relevant types of detergent compositions.
[0105] Detergent compositions comprising a variant of the invention may additionally comprise
one or more other enzymes, such as a lipase, cutinase, protease, cellulase, peroxidase
or laccase, and/or another α-amylase.
[0106] α-amylase variants of the invention may be incorporated in detergents at conventionally
employed concentrations. It is at present contemplated that a variant of the invention
may be incorporated in an amount corresponding to 0.00001-1 mg (calculated as pure,
active enzyme protein) of α-amylase per liter of wash/dishwash liquor using conventional
dosing levels of detergent.
[0107] The invention also relates to a composition comprising a mixture of one or more variants
of the invention derived from (as the parent Termamyl-like α-amylase) the
B. stearothermophilus α-amylase having the sequence shown in SEQ ID NO: 3 and a Termamyl-like alpha-amylase
derived from the
B. licheniformis α-amylase having the sequence shown in SEQ ID NO: 4.
[0108] Further, the invention also relates to a composition comprising a mixture of one
or more variants according the invention derived from (as the parent Termamyl-like
α-amylase) the
B. stearothermophilus α-amylase having the sequence shown in SEQ ID NO: 3 and a hybrid alpha-amylase comprising
a part of the
B. amyloliquefaciens α-amylase shown in SEQ ID NO: 5 and a part of the
B. licheniformis α-amylase shown in SEQ ID NO: 4. The latter mentioned hydrid Termamyl-like α-amylase
comprises the 445 C-terminal amino acid residues of the
B. licheniformis α-amylase shown in SEQ ID NO: 4 and the 37 N-terminal amino acid residues of the
α-amylase derived from
B. amyloliquefaciens shown in SEQ ID NO: 5. Said latter mentioned hybrid α-amylase may suitably comprise
the following mutations: H156Y+A181T+N190F+A209V+Q264S (using the numbering in SEQ
ID NO: 4). In the examples below said hybrid parent Termamyl-like α-amylase, is used
in combination with variants of the invention, which variants may be used in compositions
of the invention.
[0109] In a specific embodiment of the invention the composition comprises a mixture of
TVB146 and LE174, e.g., in a ratio of 2:1 to 1:2, such as 1:1.
[0110] A α-amylase variant of the invention or a composition of the invention may in an
aspect of the invention be used for washing and/or dishwashing; for textile desizing
or for starch liquefaction.
MATERIALS AND METHODS
Enzymes:
BSG alpha-amylase: B. stearothermophilus alpha-amylase depicted in SEQ ID NO: 3.
[0111] TVB146 alpha-amylase variant:
B. stearothermophilus alpha-amylase variant depicted in SEQ ID NO: 3 with the following mutations: with
the deletion in positions I181-G182 + N193F. LE174 hybrid alpha-amylase variant:
LE174 is a hybrid Termamyl-like alpha-amylase being identical to the Termamyl sequence,
i.e., the
Bacillus licheniformis α-amylase shown in SEQ ID NO: 4, except that the N-terminal 35 amino acid residues
(of the mature protein) has been replaced by the N-terminal 33 residues of BAN (mature
protein), i.e., the
Bacillus amyloliquefaciens alpha-amylase shown in SEQ ID NO: 5, which further havefollowing mutations: H156Y+A181T+N190F+A209V+Q264S
(using the numbering in SEQ ID NO: 4). LE174 was constructed by SOE-PCR (
Higuchi et al. 1988, Nucleic Acids Research 16:7351).
Fermentation and purification of α-amylase variants
[0112] A
B. subtilis strain harbouring the relevant expression plasmid is streaked on a LB-agar plate
with 10 µg/ml kanamycin from -80°C stock, and grown overnight at 37°C.
The colonies are transferred to 100 ml BPX media supplemented with 10 µg/ml kanamycin
in a 500 ml shaking flask.
Composition of BPX medium:
Potato starch |
100 g/l |
Barley flour |
50 g/l |
BAN 5000 SKB |
0.1 g/l |
Sodium caseinate |
10 g/l |
Soy Bean Meal |
20 g/l |
Na2HPO4, 12 H2O |
9 g/l |
Pluronic™ |
0.1 g/l |
[0113] The culture is shaken at 37°C at 270 rpm for 5 days.
[0114] Cells and cell debris are removed from the fermentation broth by centrifugation at
4500 rpm in 20-25 minutes. Afterwards the supernatant is filtered to obtain a completely
clear solution. The filtrate is concentrated and washed on a UF-filter (10000 cut
off membrane) and the buffer is changed to 20mM Acetate pH 5.5. The UF-filtrate is
applied on a S-sepharose F.F. and elution is carried out by step elution with 0.2M
NaCl in the same buffer. The eluate is dialysed against 10mM Tris, pH 9.0 and applied
on a Q-sepharose F.F. and eluted with a linear gradient from 0-0.3M NaCl over 6 column
volumes. The fractions which contain the activity (measured by the Phadebas assay)
are pooled, pH was adjusted to pH 7.5 and remaining color was removed by a treatment
with 0.5% W/vol. active coal in 5 minutes.
Activity determination - (KNU)
[0115] One Kilo alpah-amylase Unit (1 KNU) is the amount of enzyme which breaks down 5.26
g starch (Merck, Amylum Solubile, Erg. B 6, Batch 9947275) per hour in Novo Nordisk's
standard method for determination of alpha-amylase based upon the following condition:
Substrate |
soluble starch |
Calcium content in solvent |
0.0043 M |
Reaction time |
7-20 minutes |
Temperature |
37°C |
pH |
5.6 |
[0116] Detailed description of Novo Nordisk's analytical method (AF 9) is available on request.
BS-amylase Activity Determination - KNU(S)
1. Application Field
[0117] This method is used to determine α-amylase activity in fermentation and recovery
samples and formulated and granulated products.
2. Principle
[0118] BS-amylase breaks down the substrate (4,6-ethylidene(G
7)-p-nitrophenyl(G
1)-α,D-maltoheptaoside (written as ethylidene-G
7-PNP) into, among other things, G
2-PNP and G
3-PNP, where G denoted glucose and PNP p-nitrophenol.
[0119] G2-PNP and G3-PNP are broken down by α-glucosidase, which is added in excess, into
glucose and the yellow-coloured p-nitrophenol.
[0120] The colour reaction is monitored in situ and the change in absorbance over time calculated
as an expression of the spreed of the reaction and thus of the activity of the enzyme.
See the Boehringer Mannheim 1442 309 guidelines for further details.
2.1 Reaction conditions
[0121]
Reaction: |
Temperature : |
37°C |
pH : |
7.1 |
Pre-incubation time: |
2 minutes |
Detection: |
Wavelength : |
405 nm |
Measurement time |
3 minutes |
3. Definition of Units
[0122] Bacillus stearothermophius alpha-amylase (BS-amylase) activity is determined relative to a standard of declared
activity and stated in Kilo Novo Units (Stearothermophilus) or KNU(S)).
4. Specificity and Sensitivity
[0123] Limit of determination: approx. 0.4 KNU(s)/g
5. Apparatus
[0124] Cobas Fara analyser
Diluted (e.g. Hamilton Microlab 1000)
Analytical balance (e.g. Mettler AE 100)
Stirrer plates
6. Reagents/Substrates
[0125] A ready-made kit is used in this analysis to determine α-amylase activity. Note that
the reagents specified for the substrate and α-glucosidase are not used as described
in the Boehringer Mannheim guidelines. However, the designations "buffer", "glass
1", glass 1a" and Glass 2" are those referred to in those guidelines.
6.1. Substrate
[0126] 4,6-ethylidene(G
7)-p-nitrophenyl(G
1)-α,D-maltoheptaoside (written as ethylidene-G
7-PNP) e.g. Boehringer Mannheim 1442 309
6.2 α-glucosidase help reagent
[0127] α-glucosidase, e.g. Boehringer Mannheim 1442 309
6.3 BRIJ 35 solution
[0128]
BRIJ 35 (30% W/V Sigma 430 AG-6) |
1000 mL |
Demineralized water |
up to 2,000 mL |
6.4 Stabiliser
[0129]
Brij 35 solution |
33 mL |
CaCl2*2H2O (Merck 2382) |
882 g |
Demineralized water |
up to 2,000 mL |
7. Samples and Standards
7.1 Standard curve
Example: Preparation of BS-amylase standard curve
[0130] The relevant standard is diluted to 0.60 KNU(s)/mL as follows. A calculated quantity
of standard is weighed out and added to 200 mL volumetric flask, which is filled to
around the 2/3 mark with demineralized water. Stabiliser corresponding to 1% of the
volume of the flask is added and the flask is filled to the mark with demineralized
water.
A Hamilton Microlab 1000 is used to produce the dilutions shown below. Demineralized
water with 1% stabiliser is used as the diluent.
Dilution No. |
Enzyme stock solution |
1% stablilser |
KNU(s)/mL |
1 |
20µL |
580µL |
0.02 |
2 |
30µL |
570µL |
0.03 |
3 |
40µL |
560µL |
0.04 |
4 |
50µL |
550µL |
0.05 |
5 |
60µL |
540µL |
0.06 |
7.2 Level control
[0131] A Novo Nordisk A/S BS amylase level control is included in all runs using the Cobas
Fara. The control is diluted with 1% stabiliser so that the final dilution is within
the range of the standard curve. All weights and dilutions are noted on the worklist
7.3 Sample solutions
Single determination
[0132] Fermentation samples (not final samples) from production, all fermentation samples
from pilot plants and storage stability samples are weighed out and analyzed once
only.
Double determination over 1 run:
[0133] Process samples, final fermentation samples from production, samples from GLP studies
and R&D samples are weighed out and analyzed twice.
Double determinations over 2 runs:
[0134] Finished product samples are weighed out and analyzed twice over two separate runs.
Maximum concentration of samples in powder form: 5%
Test samples are diluted with demineralized water with 1% stabiliser to approx. 0.037
KNU(S)/mL on the basis of their expected activity. The final dilution is made direct
into the sample cup.
8. Procedure
8.1 Cobas Menu Program
[0135]
■ The Cobas Menu Program is used to suggest the weight/dilutions of samples and level
control to be used.
■ The samples are entered into the program with a unique identification code and a
worklist is printed out
■ The samples and control are weighed out and diluted as stated on the worklist with
hand-written weight data is inserted into the BS-amylase analysis logbook
■ The results are computered automatically by the Cobas Fara as described in item
9 and printed out along with the standard curve.
■ Worklists and results printouts are inserted into the BS-amylase analysis logbook.
8.2 Cobas Fara set-up
[0136]
■ The samples are placed in the sample rack
■ The five standards are placed in the calibration rack at position 1 to 5 (strongest
standard at position 5), and control placed in the same rack at position 10.
■ The substrate is transferred to a 30 mL reagent container and placed in that reagent
rack at position 2 (holder 1).
■ The α-glucosidase help reagent is transferred to a 50 mL reagent container and placed
in the reagent rack at position 2 (holder C)
8.3 Cobas Fare analysis
[0137] The main principles of the analysis are as follows:
20µL sample and 10µL rinse-water are pipetted into the cuvette along with 250µL α-glucosidase
help reagent. The cuvette rotates for 10 seconds and the reagents are thrown out into
the horizontal cuvettes. 25µL substrate and 20µL rinse-water are pipetted off. After
a 1 second wait to ensure that the temperature is 37°C, the cuvette rotates again
and the substrate is mixed into the horizontal cuvettes. Absorbance is measured for
the first time after 120 seconds and then every 5 seconds. Absorbance is measured
a total of 37 times for each sample.
9. Calculations
[0138] The activity of the samples is calculated relative to Novo Nordisk A/S standard.
The standard curve is plotted by the analyzer. The curve is to be gently curved, rising
steadily to an absorbance of around 0.25 for standard no. 5.
The activity of the samples in KNU(S)/mL is read off the standard curve by the analyzer.
The final calculations to allow for the weights/dilutions used employ the following
formula:

S= analysis result read off (KNU(S)/mL
V= volume of volumetric flask used in mL
F= dilution factor for second dilution
W= weight of enzyme sample in g
9.2 Calculation of mean values
[0139] Results are stated with 3 significant digits. However, for sample activity < 10 KNU(S)/g,
only 2 significant digits are given.
The following rules apply on calculation of mean values:
- 1. Data which deviates more than 2 standard deviations from the mean value is not
included in the calculation.
- 2. Single and double determination over one run:
The mean value is calculated on basis of results lying within the standard curve's
activity area.
- 3. Double determinations over two runs: All values are included in the mean value.
Outliers are omitted.
10. Accuracy and Precision
[0140] The coefficient of variation is 2.9% based on retrospective validation of analysis
results for a number of finished products and the level control.
Assay for α-Amylase Activity
[0141] α-Amylase activity is determined by a method employing Phadebas® tablets as substrate.
Phadebas tablets (Phadebas® Amylase Test, supplied by Pharmacia Diagnostic) contain
a crosslinked insoluble blue-coloured starch polymer which has been mixed with bovine
serum albumin and a buffer substance and tabletted.
[0142] For every single measurement one tablet is suspended in a tube containing 5 ml 50
mM Britton-Robinson buffer (50 mM acetic acid, 50 mM phosphoric acid, 50 mM boric
acid, 0.1 mM CaCl
2, pH adjusted to the value of interest with NaOH). The test is performed in a water
bath at the temperature of interest. The α-amylase to be tested is diluted in x ml
of 50 mM Britton-Robinson buffer. 1 ml of this α-amylase solution is added to the
5 ml 50 mM Britton-Robinson buffer. The starch is hydrolysed by the α-amylase giving
soluble blue fragments. The absorbance of the resulting blue solution, measured spectrophotometrically
at 620 nm, is a function of the α-amylase activity.
[0143] It is important that the measured 620 nm absorbance after 10 or 15 minutes of incubation
(testing time) is in the range of 0.2 to 2.0 absorbance units at 620 nm. In this absorbance
range there is linearity between activity and absorbance (Lambert-Beer law). The dilution
of the enzyme must therefore be adjusted to fit this criterion. Under a specified
set of conditions (temp., pH, reaction time, buffer conditions) 1 mg of a given α-amylase
will hydrolyse a certain amount of substrate and a blue colour will be produced. The
colour intensity is measured at 620 nm. The measured absorbance is directly proportional
to the specific activity (activity/mg of pure α-amylase protein) of the α-amylase
in question under the given set of conditions.
EXAMPLES
EXAMPLE 1
Construction of variants of BSG α-amylase (SEQ ID NO: 3)
[0144] The gene encoding BSG, amyS, is located in plasmid pPL1117. This plasmid contains
also the gene conferring resistance towards kanamycin and an origin of replication,
both obtained from plasmid pUB110 (
Gryczan, T.J. et al (1978) J.Bact 134:318-329).
[0145] The DNA sequence of the mature part of amyS is shown as SEQ ID NO: 11 and the amino
acid sequence of the mature protein is shown as SEQ ID NO: 3
[0146] BSG variant TVB145, which contains a deletion of 6 nucleotides corresponding to amino
acids I181-G182 in the mature protein, is constructed as follows:
[0147] Polymerase Chain Reaction (PCR) is utilized to amplify the part of the amyS gene
(from plasmid pPL1117), located between DNA primers BSG1 (SEQ ID NO: 15) and BSGM2
(SEQ ID NO: 18). BSG1 is identical to a part of the amyS gene whereas BSGM2 contains
the 6 bp nucleotide deletion. A standard PCR reaction is carried out: 94°C for 5 minutes,
25 cycles of (94°C for 45 seconds, 50°C for 45 seconds, 72°C for 90 seconds), 72°C
for 7 minutes using the Pwo polymerase under conditions as recommended by the manufacturer,
Boehringer Mannheim Gmbh.
[0149] This DNA fragment is digested with restriction endonucleases Acc65I and SalI and
the resulting approximately 550 bp fragment is ligated into plasmid pPL1117 digested
with the same enzymes and transformed into the protease- and amylase-deleted
Bacillus subtilis strain SHA273 (described in
WO92/11357 and
WO95/10603).
Kanamycin resistant and starch degrading transformants were analysed for the presence
of the desired mutations (restriction digest to verify the introduction of a HindIII
site in the gene). The DNA sequence between restriction sites Acc65I and SalI was
verified by DNA sequencing to ensure the presence of only the desired mutations.
[0150] BSG variant TVB146 which contains the same 6 nucleotide deletion as TVB145 and an
additional substitution of asparagine 193 for a phenylalanine, N193F, was constructed
in a similar way as TVB145 utilizing primer BSGM3 (SEQ ID NO: 19) in the first PCR.
[0151] BSG variant TVB161, containing the deletion of I181-G182, N193F, and L204F, is constructed
in a similar way as the two previous variants except that the template for the PCR
reactions is plasmid pTVB146 (pPL1117 containing the TVB146-mutations within amyS
and the mutagenic oligonucleotide for the first PCR is BSGM3.
[0152] BSG variant TVB162, containing the deletion of I181-G182, N193F, and E210H, is constructed
in a similar way as TVB161 except that the mutagenic oligonucleotide is BSGM4 (SEQ
ID NO: 20).
[0153] BSG variant TVB163, containing the deletion of I181-G182, N193F, and E214Q, is constructed
in a similar way as TVB161 except that the mutagenic oligonucleotide is BSGM5 (SEQ
ID NO: 21).
[0154] The above constructed BSG variants were then fermented and purified as described
above in the "Material and Methods" section.
EXAMPLE 2
Measurement of the calcium- and pH-dependent stability
[0155] Normally, the industrial liquefaction process runs using pH 6.0-6.2 as liquefaction
pH and an addition of 40 ppm free calcium in order to improve the stability at 95°C-105°C.
Some of the herein proposed substitutions have been made in order to improve the stability
at
- 1. lower pH than pH 6.2 and/or
- 2. at free calcium levels lower than 40 ppm free calcium.
[0156] Two different methods have been used to measure the improvements in stability obtained
by the different substitutions in the α-amylase from
B.stearothermophilus:
Method 1. One assay which measures the stability at reduced pH, pH 5.0, in the presence
of 5 ppm free calcium. 10 µg of the variant were incubated under the following conditions:
A 0.1 M acetate solution, pH adjusted to pH 5.0, containing 5ppm calcium and 5% w/w
common corn starch (free of calcium). Incubation was made in a water bath at 95°C
for 30 minutes.
Method 2. One assay which measure the stability in the absence of free calcium and
where the pH is maintained at pH 6.0. This assay measures the decrease in calcium
sensitivity: 10 µg of the variant were incubated under the following conditions: A
0.1 M acetate solution, pH adjusted to pH 6.0, containing 5% w/w common corn starch
(free of calcium). Incubation was made in a water bath at 95°C for 30 minutes.
Stability determination
[0157] All the stability trials 1, 2 have been made using the same set up. The method was:
[0158] The enzyme was incubated under the relevant conditions (1-4). Samples were taken
at 0, 5, 10, 15 and 30 minutes and diluted 25 times (same dilution for all taken samples)
in assay buffer (0.1M 50mM Britton buffer pH 7.3) and the activity was measured using
the Phadebas assay (Pharmacia) under standard conditions pH 7.3, 37°C.
[0159] The activity measured before incubation (0 minutes) was used as reference (100%).
The decline in percent was calculated as a function of the incubation time. The table
shows the residual activity after 30 minutes of incubation.
Stability method 1. / Low pH stability improvement
MINUTES OF INCUBATION |
WT. SEQ. ID. NO:3 AMYLASE (BSG) |
SEQ. ID NO: 3 VARIANT WITH DELETION IN POS. I181-G182 (TVB145) |
SEQ. ID NO: 3 VARIANT WITH DELETION IN POS. I181-G182 + N193F (TVB146) |
SEQ. ID NO: 3 VARIANT WITH DELETION IN POS. I181-G182 + N193F + E214Q (TVB163) |
0 |
100 |
100 |
100 |
100 |
5 |
29 |
71 |
83 |
77 |
10 |
9 |
62 |
77 |
70 |
15 |
3 |
50 |
72 |
67 |
30 |
1 |
33 |
62 |
60 |
Stability method 1. / Low pH stability improvement
[0160] The temperature describet in method 1 has been reduced from 95°C to 70°C since the
amylases mentioned for SEQ ID NO: 1 and 2 have a lower thermostability than the one
for SEQ ID NO: 3.
MINUTES OF INCUBATION |
WT. SEQ. ID. NO: 2 AMYLASE |
SEQ. ID NO: 2 VARIANT WITH DELETION IN POS. D183-G184 |
SEQ. ID NO: 1 AMYLASE |
SEQ. ID NO: 1 VARIANT WITH DELETION IN POS. T183-G184 |
0 |
100 |
100 |
100 |
100 |
5 |
73 |
92 |
41 |
76 |
10 |
59 |
88 |
19 |
69 |
15 |
48 |
91 |
11 |
62 |
30 |
28 |
92 |
3 |
59 |
Stability method 2. / Low calcium sensitivity
MINUTES OF INCUBATION |
WT. SEQ ID NO: 3 AMYLASE (BSG) |
SEQ ID NO: 3 VARIANT WITH DELETION IN POS. I181-G182 (TVB145) |
SEQ ID NO: 3 VARIANT WITH DELETION IN POS. I181-G182 + N193F (TVB146) |
SEQ ID NO: 3 VARIANT WITH DELETION IN POS. I181-G182 + N193F + E214Q (TVB163) |
0 |
100 |
100 |
100 |
100 |
5 |
60 |
82 |
81 |
82 |
10 |
42 |
/6 |
80 |
83 |
15 |
31 |
77 |
81 |
79 |
30 |
15 |
67 |
78 |
79 |
Specific activity determination.
[0161] The specific activity was determined using the Phadebas assay (Pharmacia) as activity/mg
enzyme. The activity was determined using the α-amylase assay described in the Materials
and Methods section herein.
[0162] The specific activity of the parent enzyme and a single and a double mutation was
determined to:
BSG: SEQ ID NO:3 (Parent enzyme) |
20000 NU/mg |
TVB145: SEQ ID NO:3 with the deletion in positions
I181-G182: (Single mutation) |
34600 NU/mg |
TVB146: SEQ ID NO:3 with the deletion in positions
I181-G182 + N193F: (Double mutation) |
36600 NU/mg |
TVB163: SEQ ID NO:3 with the deletion in positions
I181-G182+N193F+E214Q: (Triple mutation) |
36300 NU/mg |
EXAMPLE 3
Pilot plant jet cook and liquefaction with alpha-amylase variant TVB146
[0163] Pilot plant liquefaction experiments were run in the mini-jet system using a dosage
of 50 NU (S)/g DS at pH 5.5 with 5 ppm added Ca
++, to compare the performance of formulated BSG alpha-amylase variant TVB146 (SEQ ID
NO: 3 with deletion in positions
I181-G182 + N193F) with that of parent BSG alpha-amylase (SEQ ID NO: 3). The reaction
was monitored by measuring the DE increase (Neocuproine method) as a function of time.
[0164] Corn starch slurries were prepared by suspending 11.8 kg Cerestar C*Pharm GL 03406
(89 % starch) in deionized water and making up to 30 kg. The pH was adjusted to 5.5
at ambient temperature, after the addition of 0.55 g CaCl
2. 2H
2O.
[0165] The following enzymes were used:
TVB146 |
108 KNU(S)/g, |
146 KNU(SM9)/g |
BSG amylase |
101 KNU(S)/g, |
98 KNU(SM9)/g |
[0166] An amount of enzyme corresponding to 50 NU (SM9)/g DS was added, and the conductivity
adjusted to 300mS using NaCl. The standard conditions were as follows:
Substrate concentration |
35 % w/w (initial) |
31.6-31.9 % w/w (final) |
Temperature |
105°C, 5 min |
(Primary liquefaction) |
95°C, 90 min |
(Secondary liquefaction) |
pH (initial) |
5.5 |
|
[0167] After jetting, the liquefied starch was collected and transported in sealed thermos-flasks
from the pilot plant to the laboratory, where secondary liquefaction was continued
at 95 °C.
[0168] 10 ml samples were taken at 15 minute intervals from 15-90 minutes. 2 drops of 1
N HCl were added to inactivate the enzyme. From these samples, 0.3-0.1 g (according
to the expected DE) were weighed out and diluted to 100 ml. Reducing sugars were then
determined according to the Neocuproine method (Determination of reducing sugar with
improved precision.
Dygert, Li, Florida and Thomas (1965). Anal. Biochem 13, 368) and DE values determined. The development of DE as a function of time is given in
the following table:
|
TVB146 |
BSG |
Time (min.) |
DE (neocuproine) |
15 |
2.80 |
2.32 |
30 |
4.88 |
3.56 |
45 |
6.58 |
4.98 |
60 |
8.17 |
6.00 |
75 |
9.91 |
7.40 |
90 |
11.23 |
8.03 |
[0169] As can be seen the alpha-amylase variant TVB146 performed significantly better under
industrially relevant application conditions at low levels of calcium than the parent
BSG alpha-amylase.
EXAMPLE 4
Jet Cook and Liquefaction with a combination of alpha-amylase variants (TVB146 and
LE174)
[0170] Jet cook and liquefaction using a combination of the alpha-amylase variants, TVB146
and LE174 (ratio 1:1) were carried out at the following conditions:
Substrate |
A.E. Staley food grade powdered corn starch |
(1001bs) |
|
D.S. |
35% using DI water |
Free Ca2+ |
2.7ppm at pH 5.3 (none added, from the starch only) |
Initial pH |
5.3 |
Dose AF9 units (AF9 is available on request) for each enzyme variant was 28 NU/g starch
db for a total dose of 56 NU/g
Temperature in primary liquefaction 105°C
Hold time in primary liquefaction 5 minutes
Temperature in secondary liquefaction 95°C
[0172] The results were as follows:
Time |
DE |
15 |
3.2 |
30 |
4.8 |
45 |
6.3 |
60 |
7.8 |
75 |
9.4 |
90 |
10.4 |
127 |
13.1 |
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