(19)
(11) EP 2 208 796 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
03.11.2010 Bulletin 2010/44

(43) Date of publication A2:
21.07.2010 Bulletin 2010/29

(21) Application number: 09016058.1

(22) Date of filing: 06.01.2006
(51) International Patent Classification (IPC): 
C12Q 1/68(2006.01)
C12N 15/62(2006.01)
(84) Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

(30) Priority: 06.01.2005 US 641987 P
15.07.2005 US 699975 P

(62) Application number of the earlier application in accordance with Art. 76 EPC:
06717401.1 / 1836319

(71) Applicant: Applied Biosystems, LLC
Carlsbad, CA 92008 (US)

(72) Inventors:
  • Martin, Patrick K.
    Redwood City, CA 94061 (US)
  • Simpson, David A.
    Redwood City, CA 94062 (US)
  • Hardy, Christine D.
    Hayward, CA 94544 (US)

(74) Representative: Grund, Martin 
GRUND Intellectual Property Group Postfach 44 05 16
80754 München
80754 München (DE)

   


(54) Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification


(57) Polypeptides having nucleic acid binding activity are provided. Methods of using polypeptides having nucleic acid binding activity are provided. Fusion proteins and methods of using fusion proteins are provided. Fusion proteins comprising a polymerase and a nucleic acid binding polypeptide are provided. Fusion proteins comprising a reverse transcriptase and a nucleic acid binding polypeptide are provided. Methods are provided for amplifying a nucleic acid sequence using a fusion protein comprising a nucleic acid binding polypeptide and a polymerase. Methods are provided for amplifying a nucleic acid sequence using a fusion protein comprising a nucleic acid binding polypeptide and a reverse transcriptase.







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