| (19) |
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(11) |
EP 2 213 730 A8 |
| (12) |
CORRECTED EUROPEAN PATENT APPLICATION |
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Note: Bibliography reflects the latest situation |
| (15) |
Correction information: |
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Corrected version no 1 (W1 A1) |
| (48) |
Corrigendum issued on: |
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10.11.2010 Bulletin 2010/45 |
| (43) |
Date of publication: |
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04.08.2010 Bulletin 2010/31 |
| (22) |
Date of filing: 18.06.2003 |
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| (51) |
International Patent Classification (IPC):
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| (84) |
Designated Contracting States: |
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AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR |
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Designated Extension States: |
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AL LT LV MK |
| (30) |
Priority: |
18.06.2002 JP 2002177648
19.08.2002 JP 2002237974
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| (62) |
Application number of the earlier application in accordance with Art. 76 EPC: |
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03736235.7 / 1541674 |
| (71) |
Applicant: Eisai R&D Management Co., Ltd. |
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Tokyo 112-8088 (JP) |
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| (72) |
Inventors: |
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- Ito, Masashi
Chiba-shi, Chiba 260-0844 (JP)
- Saito, Yasushi
Chiba-shi, Chiba 260-0853 (JP)
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| (74) |
Representative: Vossius & Partner |
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Siebertstrasse 4
81675 München
81675 München (DE) |
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Remarks: |
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This application was filed on 28-04-2010 as a divisional application to the application
mentioned under INID code 62. |
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| (54) |
Primary cultured adipocytes for gene therapy |
(57) The present invention relates to primary cultured adipocytes for gene therapy, where
the adipocytes stably maintain a foreign gene encoding a protein that is secreted
outside of cells. This invention provides cells suitable for gene therapy, which can
replace bone marrow cells and liver cells used for conventional
ex vivo gene therapy. The present invention established methods for transferring foreign
genes into primary cultured adipocytes, which are suitable for ex vivo gene therapy;
can be easily collected and implanted; and can be removed after implantation. Specifically,
the present invention established these methods that use retroviral vectors. The present
invention also established primary cultured adipocytes for gene therapy, where the
adipocytes stably maintain a foreign gene encoding a protein that is secreted outside
of cells.